PMID- 25618978
OWN - NLM
STAT- MEDLINE
DCOM- 20150303
LR  - 20191027
IS  - 0255-2922 (Print)
IS  - 0255-2922 (Linking)
VI  - 34
IP  - 6
DP  - 2014 Dec
TI  - Effect of tongluojiunao injection made from sanqi (Radix Notoginseng) and zhizi 
      (Fructus Gardeniae) on brain microvascular endothelial cells and astrocytes in an 
      in vitro ischemic model.
PG  - 725-32
AB  - OBJECTIVE: To explore the effect of Tongluojiunao injection (TLJN) prepared with 
      Sanqi (Radix Notoginseng) and Zhizi (Fructus Gardeniae) on the interaction 
      between brain microvascular endothelial cells (BMECs) and astrocytes in an in 
      vitro ischemic model. METHODS: First, an in vitro model of cerebral ischemia in 
      BMECs or astrocytes was established by oxygen-glucose deprivation (OGD). TLJN was 
      used as a medicine of intervention. The OGD-injured BMECs were cultured in 
      various astrocyte-conditioned media. Cell activity, alkaline phosphatase (AKP) 
      and gamma-glutamyl transpeptidase (gamma-GT) activity, interleukin-1 beta (IL-1beta), and 
      tumor necrosis factor alpha (TNF-alpha) content in BMECs were determined. 
      Additionally, OGD-injured astrocytes were cultured in various BMEC-conditioned 
      media. Cell activity, as well as expression of brain-derived neurotrophic factor 
      (BDNF) and glial cell-derived neurotrophic factor (GDNF) in astrocytes, were 
      detected. RESULTS: The results of paracrine signaling of normal BMECs or 
      astrocytes showed a protective effect on each other: conditioned media from 
      normal astrocytes improved cell viability, AKP, and gamma-GT activity, and reduced 
      IL-1beta and TNF-alpha content of injured BMECs; conditioned media from normal BMECs 
      improved cell viability and expression of BDNF and GDNF in injured astrocytes. 
      However, once the BMECs or astrocytes were injured by OGD, the protective effect 
      decreased or disappeared. The above-mentioned protective induction was 
      effectively recovered by TLJN intervention. CONCLUSION: The therapeutic benefit 
      of TLJN was achieved by recovering two-way induction between BMECs and 
      astrocytes, enhancing activity of injured BMECs and astrocytes, stabilizing 
      enzymatic barriers, promoting expression of neurotrophic factors, and inhibiting 
      inflammatory cytokines.
FAU - Li, Weihong
AU  - Li W
FAU - Li, Xingguang
AU  - Li X
FAU - Du, Qinghong
AU  - Du Q
FAU - Li, Feng
AU  - Li F
FAU - Zhu, Yuan
AU  - Zhu Y
FAU - Liu, Yang
AU  - Liu Y
FAU - Ma, Jie
AU  - Ma J
FAU - Wan, Liangqin
AU  - Wan L
FAU - Li, Fanghe
AU  - Li F
FAU - Zhang, Sai
AU  - Zhang S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - J Tradit Chin Med
JT  - Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan
JID - 8211546
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (sanqi)
SB  - IM
MH  - Animals
MH  - Astrocytes/*drug effects/metabolism
MH  - Brain/blood supply/drug effects
MH  - Brain Ischemia/*drug therapy/metabolism
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Cells, Cultured
MH  - Drugs, Chinese Herbal/*administration & dosage
MH  - Endothelial Cells/*drug effects/metabolism
MH  - Gardenia/*chemistry
MH  - Humans
MH  - Interleukin-1beta/metabolism
MH  - Male
MH  - Microvessels/cytology/drug effects
MH  - Panax notoginseng/*chemistry
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2015/01/27 06:00
MHDA- 2015/03/04 06:00
CRDT- 2015/01/27 06:00
PHST- 2015/01/27 06:00 [entrez]
PHST- 2015/01/27 06:00 [pubmed]
PHST- 2015/03/04 06:00 [medline]
AID - 10.1016/s0254-6272(15)30088-1 [doi]
PST - ppublish
SO  - J Tradit Chin Med. 2014 Dec;34(6):725-32. doi: 10.1016/s0254-6272(15)30088-1.