PMID- 25618996
OWN - NLM
STAT- MEDLINE
DCOM- 20150414
LR  - 20220331
IS  - 0005-2086 (Print)
IS  - 0005-2086 (Linking)
VI  - 58
IP  - 4
DP  - 2014 Dec
TI  - Experimental infection of different species of birds with pigeon paramyxovirus 
      type 1 virus--evaluation of clinical outcomes, viral shedding, and distribution 
      in tissues.
PG  - 523-30
AB  - The virulence of pigeon paramyxovirus type 1 (PPMV-1) for different species of 
      birds was investigated in two independent sets of experiments in which groups of 
      pigeons, chickens, turkeys, quails, and geese (10 birds per group) were 
      inoculated with 10(6) median embryo infectious doses of PPMV-1 isolate: 1) 
      nonpassaged (nPPMV-1, intracerebral pathogenicity [ICPI] value = 1.27) and 2) 
      after six passages in specific-pathogen-free chickens (pPPMV-1, ICPI = 1.46) via 
      the oculonasal route. Naive birds were placed in contact with infected birds (two 
      birds per group) to monitor virus transmission. Clinical observation was 
      performed daily. Additionally, cloacal swabs, oropharyngeal swabs, and selected 
      organ samples were collected on days 2, 4, 7, 10, and 14 postinfection and tested 
      by real-time reverse transcriptase-PCR for estimation of viral shedding and 
      distribution in tissues. Infected pigeons exhibited nervous and digestive tract 
      symptoms, mortality, shedding, and transmission to contact birds. Chickens, 
      turkeys, quails, and geese did not exhibit any clinical signs regardless of the 
      PPMV-1 strain used for inoculation. However, in contrast to quails and geese, 
      chickens and turkeys shed the virus via the oral cavity and cloaca, and 
      transmission to contact birds was also observed. Viral RNA was identified in 
      tissues collected from all pPPMV-1-infected birds, whereas negative results were 
      obtained in the case of tissues taken from nPPMV-1-infected quails and geese. We 
      conclude that the PPMV-1 used in this study was most virulent to pigeons, 
      followed by chickens and turkeys, while quails and geese seem to have the highest 
      level of innate resistance to this strain. However, passaging of PPMV-1 in 
      chickens resulted in the increase of ICPI and noticeable but sometimes 
      contrasting changes in the replication capacities of the virus.
FAU - Smietanka, Krzysztof
AU  - Smietanka K
FAU - Olszewska, Monika
AU  - Olszewska M
FAU - Domanska-Blicharz, Katarzyna
AU  - Domanska-Blicharz K
FAU - Bocian, A Lukasz
AU  - Bocian AL
FAU - Minta, Zenon
AU  - Minta Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Avian Dis
JT  - Avian diseases
JID - 0370617
SB  - IM
MH  - Animals
MH  - Birds
MH  - Brain/virology
MH  - Cloaca/virology
MH  - Duodenum/virology
MH  - Kidney/virology
MH  - Liver/virology
MH  - Lung/virology
MH  - Newcastle Disease/*virology
MH  - Newcastle disease virus/*physiology
MH  - Real-Time Polymerase Chain Reaction/veterinary
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Species Specificity
MH  - Spleen/virology
MH  - Tissue Distribution
MH  - *Virus Shedding
EDAT- 2015/01/27 06:00
MHDA- 2015/04/15 06:00
CRDT- 2015/01/27 06:00
PHST- 2015/01/27 06:00 [entrez]
PHST- 2015/01/27 06:00 [pubmed]
PHST- 2015/04/15 06:00 [medline]
AID - 10.1637/10769-011514-Reg.1 [doi]
PST - ppublish
SO  - Avian Dis. 2014 Dec;58(4):523-30. doi: 10.1637/10769-011514-Reg.1.