PMID- 25619297
OWN - NLM
STAT- MEDLINE
DCOM- 20150722
LR  - 20181202
IS  - 1002-0098 (Print)
IS  - 1002-0098 (Linking)
VI  - 49
IP  - 12
DP  - 2014 Dec
TI  - [Effect of dentin proteoglycans on the bonding properties of dentin].
PG  - 753-7
AB  - OBJECTIVE: To investigate the potential effect of proteoglycan (PG) and 
      glycosaminoglycan (GAG) on the bonding of etch and rinse adhesive to dentin, in 
      order to improve the bonding effect of dentin. METHODS: Forty-two extracted 
      molars were used to obtain standard dentin bonding surface, and the specimens 
      were etched for 15 s with 37% phosphoric acid and divided into three groups using 
      a table of random numbers. Then the three groups undergone different incubating 
      procedures as follow: specimens in chondroitinase ABC (C-ABC) group were 
      incubated with C-ABC at 37  degrees C for 48 h in vibrator. Specimens in trypsin (TRY) 
      group were incubated with trypsin, and specimens in the control group were 
      incubated with deionized water for 48 h in the oscillators. Then specimens in 
      each group were randomly assigned into two subgroups, A (Adper(TM) Single Bond 2) 
      and B (Prime & Bond NT) (n = 7). The microtensile bond strength (microTBS), fracture 
      mode and bonding interface morphology of the specimens were evaluated via 
      microtensile testing, stereo microscope and field emission scanning electron 
      microscopy (FE-SEM) respectively after specimens being incubated in 37  degrees C water 
      for 24 h. RESULTS: The immediate microTBS of C-ABC group bonding with adhesive A and 
      B [(32.9+/-2.5) and (26.8+/-2.2) MPa] were significantly lower than that of the 
      control group [(40.7+/-3.3) and (34.6+/-3.7) MPa] (P < 0.05). While the immediate 
      microTBS of TRY group [(49.0 +/- 3.6) and (44.5 +/- 3.0) MPa] were significantly higher 
      than that of the control group(P < 0.05). CONCLUSIONS: Dentin PG participates in 
      the dentin bonding process. Removal of PG increased the immediate microTBS of dentin 
      and total etching adhesives, while removal of GAG decreased the immediate microTBS.
FAU - Gao, Yu
AU  - Gao Y
AD  - State Key Laboratory of Military Stomatology, Department of Prosthodontics, 
      School of Stomatology, The Fourth Military Medical University, Xi'an 710032, 
      China.
FAU - Zhao, Sanjun
AU  - Zhao S
FAU - Wang, Peihuan
AU  - Wang P
FAU - Lu, Shuai
AU  - Lu S
FAU - Li, Xiaojing
AU  - Li X
FAU - Chen, Jihua
AU  - Chen J
AD  - Email: jhchen@fmmu.edu.cn.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Kou Qiang Yi Xue Za Zhi
JT  - Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese 
      journal of stomatology
JID - 8711066
RN  - 0 (Dentin-Bonding Agents)
RN  - 0 (Glycosaminoglycans)
RN  - 0 (Phosphoric Acids)
RN  - 0 (Polymethacrylic Acids)
RN  - 0 (Prime & Bond)
RN  - 0 (Proteoglycans)
RN  - 0 (Resin Cements)
RN  - 0 (glucosaminoglycans)
RN  - 0 (single bond)
RN  - 454I75YXY0 (Bisphenol A-Glycidyl Methacrylate)
RN  - E4GA8884NN (phosphoric acid)
RN  - EC 3.4.21.4 (Trypsin)
RN  - EC 4.2.2.20 (Chondroitin ABC Lyase)
SB  - IM
MH  - Acid Etching, Dental/methods
MH  - Bisphenol A-Glycidyl Methacrylate/pharmacology
MH  - Chondroitin ABC Lyase/pharmacology
MH  - *Dental Bonding
MH  - Dental Stress Analysis
MH  - Dentin/*chemistry
MH  - Dentin-Bonding Agents/*pharmacology
MH  - Glycosaminoglycans/*pharmacology
MH  - Hot Temperature
MH  - Humans
MH  - Phosphoric Acids
MH  - Polymethacrylic Acids
MH  - Proteoglycans/*pharmacology
MH  - Random Allocation
MH  - Resin Cements
MH  - Tensile Strength/drug effects
MH  - Trypsin/pharmacology
EDAT- 2015/01/27 06:00
MHDA- 2015/07/23 06:00
CRDT- 2015/01/27 06:00
PHST- 2015/01/27 06:00 [entrez]
PHST- 2015/01/27 06:00 [pubmed]
PHST- 2015/07/23 06:00 [medline]
PST - ppublish
SO  - Zhonghua Kou Qiang Yi Xue Za Zhi. 2014 Dec;49(12):753-7.