PMID- 25620425
OWN - NLM
STAT- MEDLINE
DCOM- 20160519
LR  - 20240109
IS  - 1613-4133 (Electronic)
IS  - 1613-4125 (Linking)
VI  - 59
IP  - 4
DP  - 2015 Apr
TI  - The impact of dietary fibers on dendritic cell responses in vitro is dependent on 
      the differential effects of the fibers on intestinal epithelial cells.
PG  - 698-710
LID - 10.1002/mnfr.201400811 [doi]
AB  - SCOPE: In the present study, the direct interaction of commonly consumed fibers 
      with epithelial or dendritic cells (DCs) was studied. METHODS AND RESULTS: The 
      fibers were characterized for their sugar composition and chain length profile. 
      When in direct contact, fibers activate DCs only mildly. This was different when 
      DCs and fibers were co-cultured together with supernatants from human epithelial 
      cells (Caco spent medium). Caco spent medium enhanced the production of IL-12, 
      IL-1Ra, IL-6, IL-8, TNF-alpha, MCP-1 (monocyte chemotactic protein), and MIP-1alpha but 
      this was strongly attenuated by the dietary fibers. This attenuating effect on 
      proinflammatory cytokines was dependent on the interaction of the fibers with 
      Toll-like receptors as it was reduced by Pepinh-myd88. The interaction of 
      galacto-oligosaccharides, chicory inulin, wheat arabinoxylan, barley beta-glucan 
      with epithelial cells and DCs led to changes in the production of the Th1 
      cytokines in autologous T cells, while chicory inulin, and barley beta-glucan 
      reduced the Th2 cytokine IL-6. The Treg-promoting cytokine IL-10 was induced by 
      galacto-oligosaccharides whereas chicory inulin decreased the IL-10 production. 
      CONCLUSIONS: Our results suggest that dietary fibers can modulate the host immune 
      system not only by the recognized mechanism of effects on microbiota but also by 
      direct interaction with the consumer's mucosa. This modulation is dietary fiber 
      type dependent.
CI  - (c) 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Bermudez-Brito, Miriam
AU  - Bermudez-Brito M
AD  - Top Institute Food and Nutrition, Wageningen, The Netherlands; Department of 
      Pathology and Medical Biology, University Medical Center Groningen, University of 
      Groningen, Groningen, The Netherlands.
FAU - Sahasrabudhe, Neha M
AU  - Sahasrabudhe NM
FAU - Rosch, Christiane
AU  - Rosch C
FAU - Schols, Henk A
AU  - Schols HA
FAU - Faas, Marijke M
AU  - Faas MM
FAU - de Vos, Paul
AU  - de Vos P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150302
PL  - Germany
TA  - Mol Nutr Food Res
JT  - Molecular nutrition & food research
JID - 101231818
RN  - 0 (CCL2 protein, human)
RN  - 0 (CXCL8 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL3)
RN  - 0 (Dietary Fiber)
RN  - 0 (IL10 protein, human)
RN  - 0 (IL1RN protein, human)
RN  - 0 (IL6 protein, human)
RN  - 0 (Interleukin 1 Receptor Antagonist Protein)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Xylans)
RN  - 0 (beta-Glucans)
RN  - 130068-27-8 (Interleukin-10)
RN  - 187348-17-0 (Interleukin-12)
RN  - 89NA02M4RX (Pectins)
RN  - 9005-80-5 (Inulin)
RN  - 9040-27-1 (arabinoxylan)
SB  - IM
MH  - Beta vulgaris/chemistry
MH  - Caco-2 Cells
MH  - Cells, Cultured
MH  - Chemokine CCL2/metabolism
MH  - Chemokine CCL3/metabolism
MH  - Cichorium intybus/chemistry
MH  - Dendritic Cells/*drug effects
MH  - Dietary Fiber/*pharmacology
MH  - Epithelial Cells/*drug effects/metabolism
MH  - Hordeum/chemistry
MH  - Humans
MH  - Interleukin 1 Receptor Antagonist Protein/metabolism
MH  - Interleukin-10/metabolism
MH  - Interleukin-12/metabolism
MH  - Interleukin-6/metabolism
MH  - Interleukin-8/metabolism
MH  - Intestinal Mucosa/metabolism
MH  - Intestines/*cytology
MH  - Inulin/pharmacology
MH  - Pectins/pharmacology
MH  - Triticum/chemistry
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Xylans/pharmacology
MH  - beta-Glucans/pharmacology
OTO - NOTNLM
OT  - Dendritic cells
OT  - Epithelial cells
OT  - Fibers
OT  - Transwell
EDAT- 2015/01/27 06:00
MHDA- 2016/05/20 06:00
CRDT- 2015/01/27 06:00
PHST- 2014/11/06 00:00 [received]
PHST- 2015/01/15 00:00 [revised]
PHST- 2015/01/16 00:00 [accepted]
PHST- 2015/01/27 06:00 [entrez]
PHST- 2015/01/27 06:00 [pubmed]
PHST- 2016/05/20 06:00 [medline]
AID - 10.1002/mnfr.201400811 [doi]
PST - ppublish
SO  - Mol Nutr Food Res. 2015 Apr;59(4):698-710. doi: 10.1002/mnfr.201400811. Epub 2015 
      Mar 2.