PMID- 25620738
OWN - NLM
STAT- MEDLINE
DCOM- 20150602
LR  - 20210719
IS  - 1742-4658 (Electronic)
IS  - 1742-464X (Linking)
VI  - 282
IP  - 6
DP  - 2015 Mar
TI  - Overexpression of microRNA-30a inhibits hepatitis B virus X protein-induced 
      autophagosome formation in hepatic cells.
PG  - 1152-63
LID - 10.1111/febs.13209 [doi]
AB  - Hepatitis B virus (HBV) enters the host and survives by using several mechanisms. 
      One of the ways that HBV survives and replicates in the host cells is by inducing 
      autophagy. Previous reports have shown that microRNA (miRNA)-30a inhibits 
      autophagosome formation in cancer cells. Hence, we hypothesized that 
      overexpression of miRNA-30a could inhibit HBV-induced autophagosome formation in 
      hepatic cells. To study this, both HepG2 cells and HepG2.2.1.5 cells 
      (HBV-expressing stable cell line) were transfected with miRNA-30a, and the cells 
      were collected either for RNA isolation or protein isolation after 72 h of 
      transfection. Beclin-1 expression was significantly higher in untransfected 
      HepG2.2.1.5 cells than in HepG2 cells. Western blots showed that miRNA-30a 
      overexpression resulted in a significant decrease in beclin-1 expression 
      (eight-fold and four-fold in HepG2 and HepG2.2.1.5 cells, respectively) and c-myc 
      expression, whereas the numbers of terminal deoxynucleotidyl transferase-mediated 
      dUTP nick end labeling (TUNEL)-positive cells were increased. In contrast, 
      overexpression of HBV X protein (HBx) in HepG2 cells resulted in the enhancement 
      of beclin-1 (six-fold increase as compared with the empty vector-transfected 
      cells) and c-myc expression, whereas the numbers of TUNEL-positive cells were 
      reduced. To confirm these findings, HBx and miRNA-30a were coexpressed in HepG2 
      cells, and the results showed significant inhibition of autophagosome formation 
      and beclin-1 and c-myc expression, whereas apoptosis increased. These data 
      demonstrate that HBx induces autophagosome formation via beclin-1 expression, 
      whereas miRNA-30a overexpression could successfully inhibit the beclin-1 
      expression induced by HBx, thereby modulating autophagosome formation in hepatic 
      cells.
CI  - (c) 2015 FEBS.
FAU - Kumar, Satendra
AU  - Kumar S
AD  - Faculty of Life Sciences and Biotechnology, South Asian University, New Delhi, 
      India.
FAU - Gupta, Parul
AU  - Gupta P
FAU - Khanal, Sweta
AU  - Khanal S
FAU - Shahi, Aashirwad
AU  - Shahi A
FAU - Kumar, Pushpendra
AU  - Kumar P
FAU - Sarin, Shiv K
AU  - Sarin SK
FAU - Venugopal, Senthil K
AU  - Venugopal SK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150213
PL  - England
TA  - FEBS J
JT  - The FEBS journal
JID - 101229646
RN  - 0 (3' Untranslated Regions)
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (BECN1 protein, human)
RN  - 0 (Beclin-1)
RN  - 0 (MIRN30b microRNA, human)
RN  - 0 (Membrane Proteins)
RN  - 0 (MicroRNAs)
RN  - 0 (Proto-Oncogene Proteins c-myc)
RN  - 0 (Trans-Activators)
RN  - 0 (Viral Regulatory and Accessory Proteins)
RN  - 0 (hepatitis B virus X protein)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - 3' Untranslated Regions
MH  - Apoptosis
MH  - Apoptosis Regulatory Proteins/metabolism
MH  - *Autophagy
MH  - Beclin-1
MH  - Cell Proliferation
MH  - *Gene Expression Regulation
MH  - Hep G2 Cells
MH  - Hepatocytes/*metabolism
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Membrane Proteins/metabolism
MH  - MicroRNAs/*metabolism
MH  - Phagosomes/metabolism
MH  - Proto-Oncogene Proteins c-myc/metabolism
MH  - RNA/metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Trans-Activators/*metabolism
MH  - Viral Regulatory and Accessory Proteins
OTO - NOTNLM
OT  - apoptosis
OT  - autophagy
OT  - hepatitis B virus
OT  - hepatocellular carcinoma
OT  - proliferation
EDAT- 2015/01/27 06:00
MHDA- 2015/06/03 06:00
CRDT- 2015/01/27 06:00
PHST- 2014/04/30 00:00 [received]
PHST- 2015/01/12 00:00 [revised]
PHST- 2015/01/20 00:00 [accepted]
PHST- 2015/01/27 06:00 [entrez]
PHST- 2015/01/27 06:00 [pubmed]
PHST- 2015/06/03 06:00 [medline]
AID - 10.1111/febs.13209 [doi]
PST - ppublish
SO  - FEBS J. 2015 Mar;282(6):1152-63. doi: 10.1111/febs.13209. Epub 2015 Feb 13.