PMID- 2562130
OWN - NLM
STAT- MEDLINE
DCOM- 19910314
LR  - 20181130
IS  - 0004-069X (Print)
IS  - 0004-069X (Linking)
VI  - 37
IP  - 5-6
DP  - 1989
TI  - Chemotactic activity of elastin-derived peptides for human polymorphonuclear 
      leukocytes and their effect on hydrogen peroxide and myeloperoxidase release.
PG  - 741-8
AB  - The chemotactic activity of elastin-derived peptides (EP) for human 
      polymorphonuclear leukocytes (PMNL) was investigated using the under agarose 
      method. The EP were produced by digesting the bovine ligament elastin with 
      porcine pancreatic elastase. Thus prepared digest had weak chemotactic activity 
      for PMNL. The mean chemotactic index for all tested EP concentrations did not 
      exceed 1.30 and was lower than that obtained with zymosan-activated serum (ZAS, 
      n-formyl-methionyl-leucyl-phenylalanine (FMLP) 2.2 +/- 0.40, 3.1 +/- 0.32, (n = 
      10) respectively. However, EP (50 micrograms) after injection to the mouse 
      pleural cavity induced PMNL influx. The mean PMNL number found in this cavity was 
      0.09 +/- 0.03 x 10(6) for PBS and 0.18 +/- 0.03 +/- 10(6) for EP injection (p 
      less than 0.01 n = 6). Human PMNL during 60 min incubation with EP (1 to 10 
      micrograms/ml) or with EP and cytochalasin B (CB 4.8 micrograms/ml) released 
      myeloperoxidase and low amounts of hydrogen peroxide. At 1 micrograms/ml and in 
      presence of CB elastin digest was nearly as active in myeloperoxidase release as 
      FMLP (300 ng/ml). The values reached 17.1 +/- 2.5 and 19.7 +/- 2.1% of the total 
      activity of whole cell lysate, respectively. The obtained results suggest that EP 
      produced in vivo in the site of inflammation could modulate to some extent its 
      course by enhancing PMNL influx and their activation. It seems that such 
      mechanism of enhancement of the inflammatory response may occur in the lungs 
      which are rich in elastin fibers.
FAU - Nowak, D
AU  - Nowak D
AD  - Department of Pneumonology and Allergology, Medical Academy, Lodz.
FAU - Glowczynska, I
AU  - Glowczynska I
FAU - Piasecka, G
AU  - Piasecka G
LA  - eng
PT  - Journal Article
PL  - Poland
TA  - Arch Immunol Ther Exp (Warsz)
JT  - Archivum immunologiae et therapiae experimentalis
JID - 0114365
RN  - 0 (Chemotactic Factors)
RN  - 0 (Peptides)
RN  - 9007-58-3 (Elastin)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.11.1.7 (Peroxidase)
SB  - IM
MH  - Animals
MH  - *Chemotactic Factors
MH  - Chemotaxis, Leukocyte/drug effects
MH  - Elastin/*pharmacology
MH  - Humans
MH  - Hydrogen Peroxide/metabolism
MH  - In Vitro Techniques
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Neutrophils/*drug effects/physiology
MH  - Peptides/*pharmacology
MH  - Peroxidase/metabolism
EDAT- 1989/01/01 00:00
MHDA- 1989/01/01 00:01
CRDT- 1989/01/01 00:00
PHST- 1989/01/01 00:00 [pubmed]
PHST- 1989/01/01 00:01 [medline]
PHST- 1989/01/01 00:00 [entrez]
PST - ppublish
SO  - Arch Immunol Ther Exp (Warsz). 1989;37(5-6):741-8.