PMID- 25625310
OWN - NLM
STAT- MEDLINE
DCOM- 20160321
LR  - 20150224
IS  - 1791-2423 (Electronic)
IS  - 1019-6439 (Linking)
VI  - 46
IP  - 4
DP  - 2015 Apr
TI  - Prevalence of chromosomal rearrangements involving non-ETS genes in prostate 
      cancer.
PG  - 1637-42
LID - 10.3892/ijo.2015.2855 [doi]
AB  - Prostate cancer is characterized by structural rearrangements, most frequently 
      including translocations between androgen-dependent genes and members of the ETS 
      family of transcription factor like TMPRSS2:ERG. In a recent whole genome 
      sequencing study we identified 140 gene fusions that were unrelated to ETS genes 
      in 11 prostate cancers. The aim of the present study was to estimate the 
      prevalence of non-ETS gene fusions. We randomly selected 27 of these 
      rearrangements and analyzed them by fluorescence in situ hybridization (FISH) in 
      a tissue microarray format containing 500 prostate cancers. Using break-apart 
      FISH probes for one fusion partner each, we found rearrangements of 13 (48%) of 
      the 27 analyzed genes in 300-400 analyzable cancers per gene. Recurrent breakage, 
      often accompanied by partial deletion of the genes, was found for NCKAP5, SH3BGR 
      and TTC3 in 3 (0.8%) tumors each, as well as for ARNTL2 and ENOX1 in 2 (0.5%) 
      cancers each. One rearranged tumor sample was observed for each of VCL, ZNF578, 
      IMMP2L, SLC16A12, PANK1, GPHN, LRP1 and ZHX2. Balanced rearrangements, indicating 
      possible gene fusion, were found for ZNF578, SH3BGR, LPR12 and ZHX2 in individual 
      cancers only. The results of the present study confirm that rearrangements 
      involving non-ETS genes occur in prostate cancer, but demonstrate that they are 
      highly individual and typically non-recurrent.
FAU - Kluth, Martina
AU  - Kluth M
AD  - Institute of Pathology, University Medical Center Hamburg-Eppendorf, Hamburg, 
      Germany.
FAU - Galal, Rami
AU  - Galal R
AD  - Institute of Pathology, University Medical Center Hamburg-Eppendorf, Hamburg, 
      Germany.
FAU - Krohn, Antje
AU  - Krohn A
AD  - Institute of Pathology, University Medical Center Hamburg-Eppendorf, Hamburg, 
      Germany.
FAU - Weischenfeldt, Joachim
AU  - Weischenfeldt J
AD  - Genome Biology Unit, European Molecular Biology Laboratory (EMBL), D-69117 
      Heidelberg, Germany.
FAU - Tsourlakis, Christina
AU  - Tsourlakis C
AD  - Institute of Pathology, University Medical Center Hamburg-Eppendorf, Hamburg, 
      Germany.
FAU - Paustian, Lisa
AU  - Paustian L
AD  - Institute of Pathology, University Medical Center Hamburg-Eppendorf, Hamburg, 
      Germany.
FAU - Ahrary, Ramin
AU  - Ahrary R
AD  - Institute of Pathology, University Medical Center Hamburg-Eppendorf, Hamburg, 
      Germany.
FAU - Ahmed, Malik
AU  - Ahmed M
AD  - Institute of Pathology, University Medical Center Hamburg-Eppendorf, Hamburg, 
      Germany.
FAU - Scherzai, Sekander
AU  - Scherzai S
AD  - Institute of Pathology, University Medical Center Hamburg-Eppendorf, Hamburg, 
      Germany.
FAU - Meyer, Anne
AU  - Meyer A
AD  - Institute of Pathology, University Medical Center Hamburg-Eppendorf, Hamburg, 
      Germany.
FAU - Sirma, Huseyin
AU  - Sirma H
AD  - Institute of Pathology, University Medical Center Hamburg-Eppendorf, Hamburg, 
      Germany.
FAU - Korbel, Jan
AU  - Korbel J
AD  - Genome Biology Unit, European Molecular Biology Laboratory (EMBL), D-69117 
      Heidelberg, Germany.
FAU - Sauter, Guido
AU  - Sauter G
AD  - Institute of Pathology, University Medical Center Hamburg-Eppendorf, Hamburg, 
      Germany.
FAU - Schlomm, Thorsten
AU  - Schlomm T
AD  - Martini-Clinic, Prostate Cancer Center, University Medical Center 
      Hamburg-Eppendorf, Hamburg, Germany.
FAU - Simon, Ronald
AU  - Simon R
AD  - Institute of Pathology, University Medical Center Hamburg-Eppendorf, Hamburg, 
      Germany.
FAU - Minner, Sarah
AU  - Minner S
AD  - Institute of Pathology, University Medical Center Hamburg-Eppendorf, Hamburg, 
      Germany.
LA  - eng
PT  - Journal Article
DEP - 20150127
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (Proto-Oncogene Proteins c-ets)
SB  - IM
MH  - Adult
MH  - Aged
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Male
MH  - Middle Aged
MH  - Oncogene Proteins, Fusion/*genetics
MH  - Prostatic Neoplasms/*genetics/*pathology
MH  - Proto-Oncogene Proteins c-ets/genetics
MH  - Tissue Array Analysis/methods
EDAT- 2015/01/28 06:00
MHDA- 2016/03/22 06:00
CRDT- 2015/01/28 06:00
PHST- 2014/11/25 00:00 [received]
PHST- 2014/12/30 00:00 [accepted]
PHST- 2015/01/28 06:00 [entrez]
PHST- 2015/01/28 06:00 [pubmed]
PHST- 2016/03/22 06:00 [medline]
AID - 10.3892/ijo.2015.2855 [doi]
PST - ppublish
SO  - Int J Oncol. 2015 Apr;46(4):1637-42. doi: 10.3892/ijo.2015.2855. Epub 2015 Jan 
      27.