PMID- 25625585
OWN - NLM
STAT- MEDLINE
DCOM- 20151229
LR  - 20220311
IS  - 1096-0295 (Electronic)
IS  - 0273-2300 (Linking)
VI  - 71
IP  - 3
DP  - 2015 Apr
TI  - Estimating skin sensitization potency from a single dose LLNA.
PG  - 437-43
LID - S0273-2300(15)00011-2 [pii]
LID - 10.1016/j.yrtph.2015.01.009 [doi]
AB  - Skin sensitization is an important aspect of safety assessment. The mouse local 
      lymph node assay (LLNA) developed in the 1990 s is an in vivo test used for skin 
      sensitization hazard identification and characterization. More recently a reduced 
      version of the LLNA (rLLNA) has been developed as a means of identifying, but not 
      quantifying, sensitization hazard. The work presented here is aimed at enabling 
      rLLNA data to be used to give quantitative potency information that can be used, 
      inter alia, in modeling and read-across approaches to non-animal based potency 
      estimation. A probit function has been derived enabling estimation of EC3 from a 
      single dose. This has led to development of a modified version of the rLLNA, 
      whereby as a general principle the SI value at 10%, or at a lower concentration 
      if 10% is not testable, is used to calculate the EC3. This version of the rLLNA 
      has been evaluated against a selection of chemicals for which full LLNA data are 
      available, and has been shown to give EC3 values in good agreement with those 
      derived from the full LLNA.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Roberts, David W
AU  - Roberts DW
AD  - School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, 
      Byrom Street, Liverpool L3 3AF, England, United Kingdom. Electronic address: 
      D.W.Roberts@ljmu.ac.uk.
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
DEP - 20150124
PL  - Netherlands
TA  - Regul Toxicol Pharmacol
JT  - Regulatory toxicology and pharmacology : RTP
JID - 8214983
SB  - IM
MH  - Animals
MH  - Cell Proliferation/drug effects
MH  - Dermatitis, Contact/*etiology/immunology/pathology
MH  - Dose-Response Relationship, Drug
MH  - *Local Lymph Node Assay
MH  - Lymphocyte Activation/drug effects
MH  - Mice
MH  - *Models, Biological
MH  - Reproducibility of Results
MH  - Risk Assessment
MH  - Skin/*drug effects/immunology/pathology
MH  - T-Lymphocytes/drug effects/immunology
OTO - NOTNLM
OT  - Contact dermatitis
OT  - Full local lymph node assay (LLNA)
OT  - Probit analysis
OT  - Reduced LLNA
EDAT- 2015/01/28 06:00
MHDA- 2015/12/30 06:00
CRDT- 2015/01/28 06:00
PHST- 2014/10/21 00:00 [received]
PHST- 2014/12/20 00:00 [revised]
PHST- 2015/01/19 00:00 [accepted]
PHST- 2015/01/28 06:00 [entrez]
PHST- 2015/01/28 06:00 [pubmed]
PHST- 2015/12/30 06:00 [medline]
AID - S0273-2300(15)00011-2 [pii]
AID - 10.1016/j.yrtph.2015.01.009 [doi]
PST - ppublish
SO  - Regul Toxicol Pharmacol. 2015 Apr;71(3):437-43. doi: 10.1016/j.yrtph.2015.01.009. 
      Epub 2015 Jan 24.