PMID- 25626082
OWN - NLM
STAT- MEDLINE
DCOM- 20150929
LR  - 20150303
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 87
IP  - 5
DP  - 2015 Mar 3
TI  - Alleviation of electrochemical oxidation for peptides and proteins in 
      electrospray ionization: obtaining more accurate mass spectra with induced high 
      voltage.
PG  - 2727-33
LID - 10.1021/ac503990a [doi]
AB  - Accurate mass spectrometry (MS) signal for peptide/protein analysis, which could 
      be affected by various MS conditions, plays an essential role in identification 
      and quantification of biological samples. Herein, we tried to alleviate the 
      possible interferences from electrochemical oxidations during electrospray 
      ionization (ESI). Three most common electrochemical oxidation reactions in ESI 
      include oxidation of analyte, solvent, and electrode. With introduction of 
      induced electrospray ionization (IESI) (a variant form of ESI), these 
      interferences were significantly alleviated for peptides/proteins. That effect 
      was also tested with flow injection experiments with different solution flow 
      rates, electrolyte concentrations and solvent compositions, which was to simulate 
      various chromatography conditions in conventional liquid chromatography (LC) 
      separations. For all chromatography conditions tested, electrochemical oxidation 
      was significantly alleviated for the absence of physical contact between spray 
      solution and electrode.
FAU - Pei, Jiying
AU  - Pei J
AD  - Department of Chemistry, School of Chemistry and Materials Science, University of 
      Science and Technology of China , Hefei 230026, People's Republic of China.
FAU - Zhou, Xuan
AU  - Zhou X
FAU - Wang, Xiaoqun
AU  - Wang X
FAU - Huang, Guangming
AU  - Huang G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150210
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Peptide Fragments)
RN  - 0 (Proteins)
SB  - IM
MH  - Chromatography, Liquid/*methods
MH  - Electrochemistry/*methods
MH  - *Electromagnetic Fields
MH  - Humans
MH  - Oxidation-Reduction
MH  - Peptide Fragments/*chemistry
MH  - Proteins/*chemistry
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
EDAT- 2015/01/28 06:00
MHDA- 2015/09/30 06:00
CRDT- 2015/01/28 06:00
PHST- 2015/01/28 06:00 [entrez]
PHST- 2015/01/28 06:00 [pubmed]
PHST- 2015/09/30 06:00 [medline]
AID - 10.1021/ac503990a [doi]
PST - ppublish
SO  - Anal Chem. 2015 Mar 3;87(5):2727-33. doi: 10.1021/ac503990a. Epub 2015 Feb 10.