PMID- 25626968
OWN - NLM
STAT- MEDLINE
DCOM- 20150407
LR  - 20150220
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Linking)
VI  - 56
IP  - 2
DP  - 2015 Jan 27
TI  - Assessment of retinal function and morphology in aging Ccl2 knockout mice.
PG  - 1238-52
LID - 10.1167/iovs.14-15334 [doi]
AB  - PURPOSE: The chemokine Ccl2, or monocyte chemoattractant protein-1 (MCP-1), has 
      previously been identified as playing a potential role in many ocular diseases; 
      however, its role in mice is less clear. We sought to correlate changes in 
      retinal pigment epithelium (RPE) and retinal morphology with changes in function 
      in aging Ccl2(-/-) mice. METHODS: Ccl2(-/-) mice on a C57BL6J background were 
      genotyped for Crb1(rd8/rd8) and were free of this mutation. Ccl2(-/-) mice and 
      wild-type (WT) C57BL6J mice were investigated for changes in the retinal fundus 
      and histology as a function of age. The function of the rod and cone pathways, 
      and the rate of dark adaptation, was assessed using the electroretinogram (ERG) 
      up to 15 months of age. RESULTS: Fifteen-month-old Ccl2(-/-) mice had fundus 
      lesions, more subretinal microglia/macrophages, and an increase in RPE cell size, 
      indicative of RPE cell loss, when compared with WT mice. Within the retina, gross 
      morphology was normal but there was an increase in Muller cell gliosis and 
      microglial activation. These morphological changes in the Ccl2(-/-) RPE/retina 
      did not correlate with a change in either rod or cone ERG pathway function, or 
      with the rate of dark adaptation. CONCLUSIONS: These data show that Ccl2 is 
      important for preserving RPE and glial morphology with age, yet retinal function 
      and gross morphology are maintained. Altered signaling in this chemokine pathway 
      may, however, increase RPE and retinal vulnerability to disease.
CI  - Copyright 2015 The Association for Research in Vision and Ophthalmology, Inc.
FAU - Vessey, Kirstan A
AU  - Vessey KA
AD  - Department of Anatomy and Neuroscience, University of Melbourne, Melbourne, 
      Australia.
FAU - Waugh, Michelle
AU  - Waugh M
AD  - Department of Anatomy and Neuroscience, University of Melbourne, Melbourne, 
      Australia.
FAU - Jobling, Andrew I
AU  - Jobling AI
AD  - Department of Anatomy and Neuroscience, University of Melbourne, Melbourne, 
      Australia.
FAU - Phipps, Joanna A
AU  - Phipps JA
AD  - Department of Anatomy and Neuroscience, University of Melbourne, Melbourne, 
      Australia.
FAU - Ho, Tracy
AU  - Ho T
AD  - Department of Anatomy and Neuroscience, University of Melbourne, Melbourne, 
      Australia.
FAU - Trogrlic, Lidia
AU  - Trogrlic L
AD  - Department of Anatomy and Neuroscience, University of Melbourne, Melbourne, 
      Australia.
FAU - Greferath, Ursula
AU  - Greferath U
AD  - Department of Anatomy and Neuroscience, University of Melbourne, Melbourne, 
      Australia.
FAU - Fletcher, Erica L
AU  - Fletcher EL
AD  - Department of Anatomy and Neuroscience, University of Melbourne, Melbourne, 
      Australia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150127
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Chemokines)
SB  - IM
MH  - Aging/*physiology
MH  - Animals
MH  - Cells, Cultured
MH  - Chemokines/metabolism
MH  - Dark Adaptation
MH  - Disease Models, Animal
MH  - Electroretinography
MH  - Immunohistochemistry
MH  - Macular Degeneration/genetics/metabolism/*physiopathology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Retina/pathology/*physiopathology
MH  - Retinal Pigment Epithelium/metabolism/pathology/physiopathology
OTO - NOTNLM
OT  - Muller cell
OT  - age-related macular degeneration (AMD) (ARMD)
OT  - electroretinogram (ERG)
OT  - glia
OT  - macrophage
OT  - microglia
OT  - mouse
OT  - oscillatory potential
OT  - retina
OT  - retinal pigment epithelium (RPE)
EDAT- 2015/01/30 06:00
MHDA- 2015/04/08 06:00
CRDT- 2015/01/29 06:00
PHST- 2015/01/29 06:00 [entrez]
PHST- 2015/01/30 06:00 [pubmed]
PHST- 2015/04/08 06:00 [medline]
AID - iovs.14-15334 [pii]
AID - 10.1167/iovs.14-15334 [doi]
PST - epublish
SO  - Invest Ophthalmol Vis Sci. 2015 Jan 27;56(2):1238-52. doi: 10.1167/iovs.14-15334.