PMID- 25627066
OWN - NLM
STAT- MEDLINE
DCOM- 20151222
LR  - 20220317
IS  - 1532-298X (Electronic)
IS  - 1040-4651 (Print)
IS  - 1040-4651 (Linking)
VI  - 27
IP  - 1
DP  - 2015 Jan
TI  - Light-activated phytochrome A and B interact with members of the SPA family to 
      promote photomorphogenesis in Arabidopsis by reorganizing the COP1/SPA complex.
PG  - 189-201
LID - 10.1105/tpc.114.134775 [doi]
AB  - Phytochromes function as red/far-red photoreceptors in plants and are essential 
      for light-regulated growth and development. Photomorphogenesis, the developmental 
      program in light, is the default program in seed plants. In dark-grown seedlings, 
      photomorphogenic growth is suppressed by the action of the CONSTITUTIVELY 
      PHOTOMORPHOGENIC1 (COP1)/SUPPRESSOR OF phyA-105 (SPA) complex, which targets 
      positive regulators of photomorphogenic growth for degradation by the proteasome. 
      Phytochromes inhibit the COP1/SPA complex, leading to the accumulation of 
      transcription factors promoting photomorphogenesis; yet, the mechanism by which 
      they inactivate COP1/SPA is still unknown. Here, we show that light-activated 
      phytochrome A (phyA) and phytochrome B (phyB) interact with SPA1 and other SPA 
      proteins. Fluorescence resonance energy transfer-fluorescence lifetime imaging 
      microscopy analyses show that SPAs and phytochromes colocalize and interact in 
      nuclear bodies. Furthermore, light-activated phyA and phyB disrupt the 
      interaction between COP1 and SPAs, resulting in reorganization of the COP1/SPA 
      complex in planta. The light-induced stabilization of HFR1, a photomorphogenic 
      factor targeted for degradation by COP1/SPA, correlates temporally with the 
      accumulation of phyA in the nucleus and localization of phyA to nuclear bodies. 
      Overall, these data provide a molecular mechanism for the inactivation of the 
      COP1/SPA complex by phyA- and phyB-mediated light perception.
CI  - (c) 2015 American Society of Plant Biologists. All rights reserved.
FAU - Sheerin, David J
AU  - Sheerin DJ
AD  - Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany.
FAU - Menon, Chiara
AU  - Menon C
AD  - Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany Center for 
      Plant Molecular Biology, University of Tubingen, 72076 Tubingen, Germany.
FAU - zur Oven-Krockhaus, Sven
AU  - zur Oven-Krockhaus S
AD  - Center for Plant Molecular Biology, University of Tubingen, 72076 Tubingen, 
      Germany Institute of Physical and Theoretical Chemistry, University of Tubingen, 
      72076 Tubingen, Germany.
FAU - Enderle, Beatrix
AU  - Enderle B
AD  - Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany.
FAU - Zhu, Ling
AU  - Zhu L
AD  - Department of Molecular Biosciences and The Institute for Cellular and Molecular 
      Biology, The University of Texas at Austin, Austin, Texas 78712.
FAU - Johnen, Philipp
AU  - Johnen P
AD  - Center for Plant Molecular Biology, University of Tubingen, 72076 Tubingen, 
      Germany.
FAU - Schleifenbaum, Frank
AU  - Schleifenbaum F
AD  - Center for Plant Molecular Biology, University of Tubingen, 72076 Tubingen, 
      Germany Institute of Physical and Theoretical Chemistry, University of Tubingen, 
      72076 Tubingen, Germany.
FAU - Stierhof, York-Dieter
AU  - Stierhof YD
AD  - Center for Plant Molecular Biology, University of Tubingen, 72076 Tubingen, 
      Germany.
FAU - Huq, Enamul
AU  - Huq E
AUID- ORCID: 0000-0001-7692-5139
AD  - Department of Molecular Biosciences and The Institute for Cellular and Molecular 
      Biology, The University of Texas at Austin, Austin, Texas 78712.
FAU - Hiltbrunner, Andreas
AU  - Hiltbrunner A
AD  - Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany BIOSS Centre 
      for Biological Signalling Studies, University of Freiburg, 79104 Freiburg, 
      Germany andreas.hiltbrunner@biologie.uni-freiburg.de.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20150127
PL  - England
TA  - Plant Cell
JT  - The Plant cell
JID - 9208688
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Phytochrome A)
RN  - 136250-22-1 (Phytochrome B)
SB  - IM
CIN - Plant Cell. 2015 Jan;27(1):8. PMID: 25627064
MH  - Arabidopsis/*metabolism
MH  - Arabidopsis Proteins/metabolism
MH  - Fluorescence Resonance Energy Transfer
MH  - Gene Expression Regulation, Plant
MH  - Phytochrome A/*metabolism
MH  - Phytochrome B/*metabolism
MH  - Protein Binding
PMC - PMC4330587
EDAT- 2015/01/30 06:00
MHDA- 2015/12/23 06:00
PMCR- 2016/01/01
CRDT- 2015/01/29 06:00
PHST- 2015/01/29 06:00 [entrez]
PHST- 2015/01/30 06:00 [pubmed]
PHST- 2015/12/23 06:00 [medline]
PHST- 2016/01/01 00:00 [pmc-release]
AID - tpc.114.134775 [pii]
AID - 134775 [pii]
AID - 10.1105/tpc.114.134775 [doi]
PST - ppublish
SO  - Plant Cell. 2015 Jan;27(1):189-201. doi: 10.1105/tpc.114.134775. Epub 2015 Jan 
      27.