PMID- 25627067
OWN - NLM
STAT- MEDLINE
DCOM- 20151222
LR  - 20220408
IS  - 1532-298X (Electronic)
IS  - 1040-4651 (Print)
IS  - 1040-4651 (Linking)
VI  - 27
IP  - 1
DP  - 2015 Jan
TI  - Two distinct domains of the UVR8 photoreceptor interact with COP1 to initiate 
      UV-B signaling in Arabidopsis.
PG  - 202-13
LID - 10.1105/tpc.114.133868 [doi]
AB  - UV-B photon reception by the Arabidopsis thaliana homodimeric UV RESISTANCE 
      LOCUS8 (UVR8) photoreceptor leads to its monomerization and a crucial interaction 
      with CONSTITUTIVELY PHOTOMORPHOGENIC1 (COP1). Relay of the subsequent signal 
      regulates UV-B-induced photomorphogenesis and stress acclimation. Here, we report 
      that two separate domains of UVR8 interact with COP1: the beta-propeller domain of 
      UVR8 mediates UV-B-dependent interaction with the WD40 repeats-based predicted 
      beta-propeller domain of COP1, whereas COP1 activity is regulated by interaction 
      through the UVR8 C-terminal C27 domain. We show not only that the C27 domain is 
      required for UVR8 activity but also that chemically induced expression of the C27 
      domain is sufficient to mimic UV-B signaling. We further show, in contrast with 
      COP1, that the WD40 repeat proteins REPRESSOR OF UV-B PHOTOMORPHOGENESIS1 (RUP1) 
      and RUP2 interact only with the UVR8 C27 domain. This coincides with their 
      facilitation of UVR8 reversion to the ground state by redimerization and their 
      potential to interact with UVR8 in a UV-B-independent manner. Collectively, our 
      results provide insight into a key mechanism of photoreceptor-mediated signaling 
      and its negative feedback regulation.
CI  - (c) 2015 American Society of Plant Biologists. All rights reserved.
FAU - Yin, Ruohe
AU  - Yin R
AD  - Department of Botany and Plant Biology, University of Geneva, Sciences III, 
      CH-1211 Geneva 4, Switzerland.
FAU - Arongaus, Adriana B
AU  - Arongaus AB
AD  - Department of Botany and Plant Biology, University of Geneva, Sciences III, 
      CH-1211 Geneva 4, Switzerland.
FAU - Binkert, Melanie
AU  - Binkert M
AD  - Department of Botany and Plant Biology, University of Geneva, Sciences III, 
      CH-1211 Geneva 4, Switzerland.
FAU - Ulm, Roman
AU  - Ulm R
AD  - Department of Botany and Plant Biology, University of Geneva, Sciences III, 
      CH-1211 Geneva 4, Switzerland roman.ulm@unige.ch.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150127
PL  - England
TA  - Plant Cell
JT  - The Plant cell
JID - 9208688
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Photoreceptors, Plant)
RN  - EC 2.3.2.27 (AT2G32950 protein, Arabidopsis)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
MH  - Arabidopsis/*metabolism
MH  - Arabidopsis Proteins/*metabolism
MH  - Gene Expression Regulation, Plant
MH  - Photoreceptors, Plant/*metabolism
MH  - Protein Binding
MH  - Signal Transduction/radiation effects
MH  - Ubiquitin-Protein Ligases
MH  - *Ultraviolet Rays
PMC - PMC4330580
EDAT- 2015/01/30 06:00
MHDA- 2015/12/23 06:00
PMCR- 2015/01/27
CRDT- 2015/01/29 06:00
PHST- 2015/01/29 06:00 [entrez]
PHST- 2015/01/30 06:00 [pubmed]
PHST- 2015/12/23 06:00 [medline]
PHST- 2015/01/27 00:00 [pmc-release]
AID - tpc.114.133868 [pii]
AID - 133868 [pii]
AID - 10.1105/tpc.114.133868 [doi]
PST - ppublish
SO  - Plant Cell. 2015 Jan;27(1):202-13. doi: 10.1105/tpc.114.133868. Epub 2015 Jan 27.