PMID- 25627950
OWN - NLM
STAT- MEDLINE
DCOM- 20150408
LR  - 20150128
IS  - 1538-5159 (Electronic)
IS  - 0017-9078 (Linking)
VI  - 108
IP  - 3
DP  - 2015 Mar
TI  - Assessing the applicability of FISH-based prematurely condensed dicentric 
      chromosome assay in triage biodosimetry.
PG  - 371-6
LID - 10.1097/HP.0000000000000182 [doi]
AB  - The dicentric chromosome assay (DCA) has been regarded as the gold standard of 
      radiation biodosimetry. The assay, however, requires a 2-d peripheral blood 
      lymphocyte culture before starting metaphase chromosome analyses to estimate 
      biological doses. Other biological assays also have drawbacks with respect to the 
      time needed to obtain dose estimates for rapid decision on the correct line of 
      medical treatment. Therefore, alternative technologies that suit requirements for 
      triage biodosimetry are needed. Radiation-induced DNA double strand breaks in G0 
      lymphocytes can be detected as interphase chromosome aberrations by the cell 
      fusion-mediated premature chromosome condensation (PCC) method. The method, in 
      combination with fluorescence in situ hybridization (FISH) techniques, has been 
      proposed in early studies as a powerful tool for obtaining biological dose 
      estimates without 2-d lymphocyte culture procedures. The present work assesses 
      the applicability of FISH-based PCC techniques using pan-centromeric and 
      telomeric peptide nucleic acid (PNA) probes in triage mode biodosimetry and 
      demonstrates that an improved rapid procedure of the prematurely condensed 
      dicentric chromosome (PCDC) assay has the potential for evaluating exposed 
      radiation doses in as short as 6 h after the collection of peripheral blood 
      specimens.
FAU - Suto, Yumiko
AU  - Suto Y
AD  - *Research Center for Radiation Emergency Medicine, National Institute of 
      Radiological Sciences, Chiba, Japan; daggerRadiation Emergency Medical Assistance 
      Team, National Institute of Radiological Sciences, Chiba, Japan; double daggerDepartment of 
      Health Sciences, University College of North Atlantic, Doha, State of Qatar.
FAU - Gotoh, Takaya
AU  - Gotoh T
FAU - Noda, Takashi
AU  - Noda T
FAU - Akiyama, Miho
AU  - Akiyama M
FAU - Owaki, Makiko
AU  - Owaki M
FAU - Darroudi, Firouz
AU  - Darroudi F
FAU - Hirai, Momoki
AU  - Hirai M
LA  - eng
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Health Phys
JT  - Health physics
JID - 2985093R
SB  - IM
MH  - Adult
MH  - Chromosome Aberrations/*radiation effects
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Radiometry/*methods
MH  - Time Factors
MH  - Triage/*methods
EDAT- 2015/01/30 06:00
MHDA- 2015/04/09 06:00
CRDT- 2015/01/29 06:00
PHST- 2015/01/29 06:00 [entrez]
PHST- 2015/01/30 06:00 [pubmed]
PHST- 2015/04/09 06:00 [medline]
AID - 00004032-201503000-00010 [pii]
AID - 10.1097/HP.0000000000000182 [doi]
PST - ppublish
SO  - Health Phys. 2015 Mar;108(3):371-6. doi: 10.1097/HP.0000000000000182.