PMID- 25628757
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150128
LR  - 20240322
IS  - 1755-8166 (Print)
IS  - 1755-8166 (Electronic)
IS  - 1755-8166 (Linking)
VI  - 8
IP  - 1
DP  - 2015
TI  - Genomic instability in complicated and uncomplicated Egyptian schistosomiasis 
      haematobium patients.
PG  - 1
LID - 10.1186/s13039-014-0104-5 [doi]
LID - 1
AB  - BACKGROUND: Exploration of genetic changes during active Schistosoma infection is 
      important for anticipation and prevention of chronic sequelae. This study aimed 
      to explore the genomic instability in chromosomal and cellular kinetics in 
      Egyptians suffering from uncomplicated active schistosomiasis haematobium 
      infection in addition to chronic schistosomiasis haematobium cases complicated by 
      bilharzial-associated bladder cancer (BAC). RESULTS: This study was conducted on 
      46 schistosomiasis haemotobium cases, 22 were active (Viable S. haematobium eggs 
      in urine samples as detected by microscopy) and 24 were chronic complicated with 
      bladder cancer. Three cytogenetic techniques were applied; the first was 
      quantitative nuclear-morphocytometry by means of which the Feulgen-stained nuclei 
      were analyzed for parameters including shape, size, integrated optical-density 
      and nuclear area. The second was Fluorescent In-Situ Hybridization (FISH) for 
      specific p53gene-locus of chromosome 17 and the third technique was karyotyping. 
      Concerning chronic complicated cases, the mean +/- SD of DNA-content in urinary 
      bladder tissue sections was 3.18 +/- 0.65. Five samples (20.83%) of bladder tissue 
      sections of chronic complicated cases showed diploid nuclei, 6 urinary bladder 
      tissue samples (25%) were tetraploid, while 13 bladder samples (54.16%) were 
      aneuploid. Epithelial cells of urine samples demonstrated aneuploidy 
      (mean +/- SD = 3.74 +/- 0.36).Nuclear contents showed high proliferative DNA index in 
      all urinary epithelial cells. In the acute uncomplicated group, nuclear-DNA of 
      urinary epithelial cells was found diploid with mean nuclear-DNA content of 
      2.2 +/- 0.16SD. Half of these diploid smears had a high proliferation index. The 
      difference between nuclear DNA-contents in acute and chronic cases was 
      significant (P = 0.0001). FISH technique for specific p53gene-locus and 
      karyotyping were done on urinary bladder tissue specimens and peripheral blood 
      monocytes of 8 chronic cases respectively. Three samples (37.5%) with invasive 
      BAC had a deletion of the p53 gene. Karyotyping showed three cases out of the 8 
      chronic schistosomiasis haematobium patients with chromosomal fragmentations. 
      CONCLUSIONS: DNA morphometry was valuable in detection of gross genetic changes 
      in urothelial tissues. It is an important prognostic factor in established 
      schistosomiasis haematobium induced bladder malignancy. It has the great 
      advantage of being applicable on urine cells making it suitable for the 
      prediction of a tendency towards genetic instability in active schistosomiasis 
      haematobium patients.
FAU - Abd El-Aal, Amany A
AU  - Abd El-Aal AA
AD  - Parasitology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
FAU - Bayoumy, Ibrahim R
AU  - Bayoumy IR
AD  - Parasitology Department, Theodor Bilharz Research Institute, Giza, Egypt.
FAU - Basyoni, Maha M A
AU  - Basyoni MM
AD  - Parasitology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
FAU - Abd El-Aal, Asmaa A
AU  - Abd El-Aal AA
AD  - Clinical Pathology Department, Faculty of Medicine, Cairo University, Cairo, 
      Egypt.
FAU - Emran, Ashraf M
AU  - Emran AM
AD  - Urosurgery Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
FAU - Abd El-Tawab, Magda S
AU  - Abd El-Tawab MS
AD  - Parasitology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
FAU - Badawi, Manal A
AU  - Badawi MA
AD  - Pathology Department, National Research Center, Giza, Egypt.
FAU - Zalat, Rabab M
AU  - Zalat RM
AD  - Parasitology Department, Theodor Bilharz Research Institute, Giza, Egypt.
FAU - Diab, Tarek M
AU  - Diab TM
AD  - Parasitology Department, Theodor Bilharz Research Institute, Giza, Egypt.
LA  - eng
PT  - Journal Article
DEP - 20150122
PL  - England
TA  - Mol Cytogenet
JT  - Molecular cytogenetics
JID - 101317942
PMC - PMC4307227
OTO - NOTNLM
OT  - Chromosomal abnormalities
OT  - FISH
OT  - Karyotyping
OT  - Morphocytometry
OT  - Schistosomiasis haematobium
EDAT- 2015/01/30 06:00
MHDA- 2015/01/30 06:01
PMCR- 2015/01/22
CRDT- 2015/01/29 06:00
PHST- 2014/10/16 00:00 [received]
PHST- 2014/12/22 00:00 [accepted]
PHST- 2015/01/29 06:00 [entrez]
PHST- 2015/01/30 06:00 [pubmed]
PHST- 2015/01/30 06:01 [medline]
PHST- 2015/01/22 00:00 [pmc-release]
AID - 104 [pii]
AID - 10.1186/s13039-014-0104-5 [doi]
PST - epublish
SO  - Mol Cytogenet. 2015 Jan 22;8(1):1. doi: 10.1186/s13039-014-0104-5. eCollection 
      2015.