PMID- 25629558
OWN - NLM
STAT- MEDLINE
DCOM- 20151007
LR  - 20181113
IS  - 2072-6643 (Electronic)
IS  - 2072-6643 (Linking)
VI  - 7
IP  - 2
DP  - 2015 Jan 26
TI  - Nutritional ingredients modulate adipokine secretion and inflammation in human 
      primary adipocytes.
PG  - 865-86
LID - 10.3390/nu7020865 [doi]
AB  - Nutritional factors such as casein hydrolysates and long chain polyunsaturated 
      fatty acids have been proposed to exert beneficial metabolic effects. We aimed to 
      investigate how a casein hydrolysate (eCH) and long chain polyunsaturated fatty 
      acids could affect human primary adipocyte function in vitro. Incubation 
      conditions with the different nutritional factors were validated by assessing 
      cell vitality with lactate dehydrogenase (LDH) release and neutral red 
      incorporation. Intracellular triglyceride content was assessed with Oil Red O 
      staining. The effect of eCH, a non-peptidic amino acid mixture (AA), and 
      long-chain polyunsaturated fatty acids (LC-PUFAs) on adiponectin and leptin 
      secretion was determined by enzyme-linked immunosorbent assay (ELISA). 
      Intracellular adiponectin expression and nuclear factor-kappaB (NF-kappaB) activation 
      were analyzed by Western blot, while monocyte chemoattractant protein-1 (MCP-1) 
      release was explored by ELISA. The eCH concentration dependently increased 
      adiponectin secretion in human primary adipocytes through its intrinsic peptide 
      bioactivity, since the non-peptidic mixture, AA, could not mimic eCH's effects on 
      adiponectin secretion. Eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), 
      and DHA combined with arachidonic acid (ARA) upregulated adiponectin secretion. 
      However, only DHA and DHA/ARA exerted a potentanti-inflammatory effect reflected 
      by prevention of tumor necrosis factor-alpha (TNF-alpha) induced NF-kappaB activation and 
      MCP-1 secretion in human adipocytes. eCH and DHA alone or in combination with 
      ARA, may hold the key for nutritional programming through their anti-inflammatory 
      action to prevent diseases with low-grade chronic inflammation such as obesity or 
      diabetes.
FAU - Romacho, Tania
AU  - Romacho T
AD  - Paul Langerhans Group for Integrative Physiology, German Diabetes Center, Auf'm 
      Hennekamp 65, 40225 Dusseldorf, Germany. tania.romacho@ddz.uni-duesseldorf.de.
FAU - Glosse, Philipp
AU  - Glosse P
AD  - Paul Langerhans Group for Integrative Physiology, German Diabetes Center, Auf'm 
      Hennekamp 65, 40225 Dusseldorf, Germany. Philipp_Glosse@gmx.de.
FAU - Richter, Isabel
AU  - Richter I
AD  - Paul Langerhans Group for Integrative Physiology, German Diabetes Center, Auf'm 
      Hennekamp 65, 40225 Dusseldorf, Germany. richter_isabel@gmx.de.
FAU - Elsen, Manuela
AU  - Elsen M
AD  - Paul Langerhans Group for Integrative Physiology, German Diabetes Center, Auf'm 
      Hennekamp 65, 40225 Dusseldorf, Germany. manuela.elsen@ddz.uni-duesseldorf.de.
FAU - Schoemaker, Marieke H
AU  - Schoemaker MH
AD  - Global Discovery, Mead Johnson Pediatric Nutrition Institute, Middenkampweg 2, 
      Nijmegen, 6545 CJ, The Netherlands. Marieke.Schoemaker@mjn.com.
FAU - van Tol, Eric A
AU  - van Tol EA
AD  - Global Discovery, Mead Johnson Pediatric Nutrition Institute, Middenkampweg 2, 
      Nijmegen, 6545 CJ, The Netherlands. Ric.VanTol@mjn.com.
FAU - Eckel, Jurgen
AU  - Eckel J
AD  - Paul Langerhans Group for Integrative Physiology, German Diabetes Center, Auf'm 
      Hennekamp 65, 40225 Dusseldorf, Germany. eckel@uni-duesseldorf.de.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150126
PL  - Switzerland
TA  - Nutrients
JT  - Nutrients
JID - 101521595
RN  - 0 (Adipokines)
RN  - 0 (CCL2 protein, human)
RN  - 0 (Caseins)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Fatty Acids, Unsaturated)
RN  - 0 (Leptin)
RN  - 0 (Milk Proteins)
RN  - 0 (NF-kappa B)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 25167-62-8 (Docosahexaenoic Acids)
RN  - 65072-00-6 (casein hydrolysate)
RN  - AAN7QOV9EA (Eicosapentaenoic Acid)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
SB  - IM
MH  - Adipocytes/drug effects/*metabolism
MH  - Adipokines/*metabolism
MH  - Caseins/*metabolism
MH  - Cell Culture Techniques
MH  - Chemokine CCL2/metabolism
MH  - Docosahexaenoic Acids/metabolism
MH  - Eicosapentaenoic Acid/metabolism
MH  - Fatty Acids, Unsaturated/*metabolism
MH  - Humans
MH  - Inflammation/*metabolism
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Leptin/metabolism
MH  - Milk Proteins/*metabolism
MH  - NF-kappa B/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
PMC - PMC4344565
EDAT- 2015/01/30 06:00
MHDA- 2015/10/08 06:00
PMCR- 2015/02/01
CRDT- 2015/01/29 06:00
PHST- 2014/11/08 00:00 [received]
PHST- 2015/01/16 00:00 [accepted]
PHST- 2015/01/29 06:00 [entrez]
PHST- 2015/01/30 06:00 [pubmed]
PHST- 2015/10/08 06:00 [medline]
PHST- 2015/02/01 00:00 [pmc-release]
AID - nu7020865 [pii]
AID - nutrients-07-00865 [pii]
AID - 10.3390/nu7020865 [doi]
PST - epublish
SO  - Nutrients. 2015 Jan 26;7(2):865-86. doi: 10.3390/nu7020865.