PMID- 25631010
OWN - NLM
STAT- MEDLINE
DCOM- 20151007
LR  - 20150129
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1264
DP  - 2015
TI  - mTRIP: an imaging tool to investigate mitochondrial DNA dynamics in physiology 
      and disease at the single-cell resolution.
PG  - 133-47
LID - 10.1007/978-1-4939-2257-4_13 [doi]
AB  - Mitochondrial physiology and metabolism are closely linked to replication and 
      transcription of the genome of the organelle, the mitochondrial DNA (mtDNA). 
      However, the characterization of mtDNA processing is poorly defined at the 
      single-cell level. Here, we describe mTRIP (mitochondrial transcription and 
      replication imaging protocol), an imaging approach based on modified fluorescence 
      in situ hybridization (FISH), which simultaneously reveals mitochondrial 
      structures engaged in mtDNA initiation of replication and global mitochondrial 
      RNA (mtRNA) content at the single-cell level in human cells. In addition, mTRIP 
      can be coupled to immunofluorescence for in situ protein tracking, or to 
      MitoTracker, thereby allowing simultaneous labelling of mtDNA, mtRNA, and 
      proteins or mitochondria, respectively. Altogether, qualitative and quantitative 
      alterations of the dynamics of mtDNA processing are detected by mTRIP in human 
      cells undergoing physiological changes, as well as stress and dysfunction, with a 
      potential for diagnostic of mitochondrial diseases.
FAU - Chatre, Laurent
AU  - Chatre L
AD  - Team "Stability of Nuclear and Mitochondrial," Unite de Genetique Moleculaire des 
      Levures, Institut Pasteur, CNRS UMR3525, 25-28 Rue du Dr. Roux, Paris, France, 
      laurent.chatre@pasteur.fr.
FAU - Ricchetti, Miria
AU  - Ricchetti M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (DNA, Mitochondrial)
SB  - IM
MH  - Cell Line, Transformed
MH  - *DNA Replication
MH  - DNA, Mitochondrial/chemistry/*genetics/isolation & purification/metabolism
MH  - Genome, Mitochondrial
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - Microscopy, Fluorescence/instrumentation/methods
MH  - Mitochondrial Diseases/*genetics/metabolism
MH  - Molecular Imaging/instrumentation/*methods
MH  - Nucleic Acid Hybridization/methods
MH  - Single-Cell Analysis/instrumentation/*methods
MH  - *Transcription, Genetic
EDAT- 2015/01/30 06:00
MHDA- 2015/10/08 06:00
CRDT- 2015/01/30 06:00
PHST- 2015/01/30 06:00 [entrez]
PHST- 2015/01/30 06:00 [pubmed]
PHST- 2015/10/08 06:00 [medline]
AID - 10.1007/978-1-4939-2257-4_13 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2015;1264:133-47. doi: 10.1007/978-1-4939-2257-4_13.