PMID- 25631264
OWN - NLM
STAT- MEDLINE
DCOM- 20151019
LR  - 20181113
IS  - 1573-4838 (Electronic)
IS  - 0957-4530 (Linking)
VI  - 26
IP  - 2
DP  - 2015 Feb
TI  - Investigation of the antimicrobial activity and biocompatibility of magnesium 
      alloy coated with HA and antimicrobial peptide.
PG  - 66
LID - 10.1007/s10856-015-5389-3 [doi]
AB  - Implant-associated infection is one of the biggest problems in orthopedic 
      surgery. Antimicrobial peptides (AMPs) are well-known components of the innate 
      immunity and less susceptible to the development of pathogen resistance compared 
      to conventional antibiotics. Magnesium alloys as potential biodegradable bone 
      implants have been received much attention in biomaterials field. This study 
      investigated the deposition of calcium phosphate (CaP) coatings and loading of 
      AMPs on the magnesium alloy surface by a biomimetic method. Scanning electron 
      microscope (SEM) results presented that a microporous and plate-like CaP coating 
      was processed on the magnesium alloy surface. X-ray diffractometry (XRD) and 
      Fourier transform infrared spectroscopy (FTIR) analysis showed the main component 
      of coating was hydroxyapatite (HA). Degradation assay in vitro showed that the HA 
      coating deposited onto the magnesium alloy was corroded more slowly than the bare 
      one. The amount of AMP loaded in the HA coating was 11.16+/-1.99 mug/cm2. The AMP 
      loaded onto HA coatings had slow release for 7 days. The AMP-loaded coating 
      showed antimicrobial activity against Staphylococcus aureus. Its bacterial 
      inhibition rate exceeded 50% after 4 days and the antibacterial effect was 
      sustained for 7 days. The coated magnesium alloys loaded with AMP could improve 
      rat bone marrow mesenchymal stem cells (rBMMSCs) proliferation. Furthermore, it 
      could also promote alkaline phosphatase (ALP) activity of rBMMSCs. Both 
      radiographic evaluation and histopathology analysis demonstrated that 
      implantation of the coated magnesium alloy into the rabbit femoral condyle had 
      promoted bone repair and showed anti-inflammatory effect. The results showed that 
      the AMP loaded onto HA coatings on the magnesium alloy surface could be 
      considered an ideal orthopedic implant against S. aureus infection.
FAU - Tian, Jinhuan
AU  - Tian J
AD  - Department of Materials Science and Engineering, Jinan University, Guangzhou, 
      510630, China.
FAU - Shen, Si
AU  - Shen S
FAU - Zhou, Changren
AU  - Zhou C
FAU - Dang, Xiangli
AU  - Dang X
FAU - Jiao, Yanpeng
AU  - Jiao Y
FAU - Li, Lihua
AU  - Li L
FAU - Ding, Shan
AU  - Ding S
FAU - Li, Hong
AU  - Li H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150129
PL  - United States
TA  - J Mater Sci Mater Med
JT  - Journal of materials science. Materials in medicine
JID - 9013087
RN  - 0 (Alloys)
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Antimicrobial Cationic Peptides)
RN  - 0 (Coated Materials, Biocompatible)
RN  - 91D9GV0Z28 (Durapatite)
RN  - I38ZP9992A (Magnesium)
SB  - IM
MH  - Adsorption
MH  - Alloys/chemistry
MH  - Anti-Bacterial Agents/chemistry/pharmacology
MH  - Antimicrobial Cationic Peptides/*administration & dosage/*chemistry
MH  - Apoptosis/drug effects/physiology
MH  - Cell Survival/drug effects/physiology
MH  - Coated Materials, Biocompatible/administration & dosage/*chemical synthesis
MH  - Durapatite/*chemistry
MH  - Magnesium/*chemistry
MH  - Materials Testing
MH  - Staphylococcus aureus/*drug effects/physiology
EDAT- 2015/01/30 06:00
MHDA- 2015/10/20 06:00
CRDT- 2015/01/30 06:00
PHST- 2014/07/01 00:00 [received]
PHST- 2014/09/26 00:00 [accepted]
PHST- 2015/01/30 06:00 [entrez]
PHST- 2015/01/30 06:00 [pubmed]
PHST- 2015/10/20 06:00 [medline]
AID - 10.1007/s10856-015-5389-3 [doi]
PST - ppublish
SO  - J Mater Sci Mater Med. 2015 Feb;26(2):66. doi: 10.1007/s10856-015-5389-3. Epub 
      2015 Jan 29.