PMID- 25631502
OWN - NLM
STAT- MEDLINE
DCOM- 20150430
LR  - 20150129
IS  - 1011-601X (Print)
IS  - 1011-601X (Linking)
VI  - 28
IP  - 1 Suppl
DP  - 2015 Jan
TI  - Purification of procyanidins from Kunlun Chrysanthemum by macroporous resins 
      combined with silica gel and evaluation of antioxidant activities in vitro.
PG  - 383-91
AB  - The objective of the present study was to evaluate an efficient purifying process 
      of Kunlun Chrysanthemum procyanidins (KCPC) by combination of AB-8 macroporous 
      adsorption resin and silica gel column through adsorption and desorption 
      experiments under static and dynamic status with the purity of procyanidins and 
      the antioxidant activity as indexes respectively. The optimum parameters for 
      adsorption by AB-8 resin were as follows: Sample concentration 1mg/mL, pH6, 
      injecting velocity 2 bed volume (BV) /h, with 3 BV 70% ethanol as eluting 
      solvent, and elution flow rate 2BV /h. One cycle after treatment using AB-8 
      resin, the purity of KCPC obviously enhanced 3 times compared with that of not 
      prepurified, that is from 22.68 to 63.76%. The optimum parameters for adsorption 
      by silica gel column were as follows: The concentration of procyanidins in a 
      sample solution of 1.2mg/mL (pH 6) with a speed of 2BV/h. Concentration for 
      desorption, with 5 BV of 80% ethanol as an eluent at a flow rate of 2 BV/h. After 
      one-run treatment with silica gel column, the purity of KCPC increased from 63.76 
      to 81.97%. The antioxidant activities of the purified KCPC in vitro were further 
      investigated. The results indicated that the purification method of combination 
      of AB-8 resin and silica gel column was superior to AB-8 adsorption resin used 
      alone in term of antioxidant activities. Moreover, the 
      1,1-diphenyl-2-picrylhydrazyl (DPPH*) scavenging ability, the scavenging activity 
      of hydroxyl radicals and the reducing ability appeared to be dose-dependent of 
      KCPC. The novel purification method of combination of AB-8 resin and silica gel 
      column will offer a promising way to purify KCPC for wider application.
FAU - Jing, Siqun
AU  - Jing S
AD  - 1State Key Laboratory of Food Science and Technology, School of Food Science and 
      Technology, Jiangnan University, Lihu Road, Wuxi, Jiangsu, China / College of 
      Life Sciences and Technology, Xinjiang University, Shengli Road 14, Urumqi, 
      Xinjiang, China.
FAU - Zhang, Xiaoming
AU  - Zhang X
AD  - State Key Laboratory of Food Science and Technology, School of Food Science and 
      Technology, Jiangnan University, Lihu Road, Wuxi, Jiangsu, China.
FAU - Yue, Li
AU  - Yue L
AD  - College of Life Sciences and Technology, Xinjiang University, Shengli Road 14, 
      Urumqi, Xinjiang, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Pakistan
TA  - Pak J Pharm Sci
JT  - Pakistan journal of pharmaceutical sciences
JID - 9426356
RN  - 0 (Antioxidants)
RN  - 0 (Free Radical Scavengers)
RN  - 0 (Proanthocyanidins)
RN  - 0 (Resins, Synthetic)
RN  - 60650-90-0 (Silica Gel)
SB  - IM
MH  - Adsorption
MH  - Antioxidants/*pharmacology
MH  - Chrysanthemum/*chemistry
MH  - Free Radical Scavengers/pharmacology
MH  - Hydrogen-Ion Concentration
MH  - Porosity
MH  - Proanthocyanidins/*isolation & purification/pharmacology
MH  - Resins, Synthetic/*chemistry
MH  - Silica Gel/chemistry
EDAT- 2015/01/30 06:00
MHDA- 2015/05/01 06:00
CRDT- 2015/01/30 06:00
PHST- 2015/01/30 06:00 [entrez]
PHST- 2015/01/30 06:00 [pubmed]
PHST- 2015/05/01 06:00 [medline]
PST - ppublish
SO  - Pak J Pharm Sci. 2015 Jan;28(1 Suppl):383-91.