PMID- 25632160
OWN - NLM
STAT- MEDLINE
DCOM- 20160310
LR  - 20150316
IS  - 1477-9137 (Electronic)
IS  - 0021-9533 (Linking)
VI  - 128
IP  - 6
DP  - 2015 Mar 15
TI  - Myosin Va mediates BDNF-induced postendocytic recycling of full-length TrkB and 
      its translocation into dendritic spines.
PG  - 1108-22
LID - 10.1242/jcs.160259 [doi]
AB  - Brain-derived neurotrophic factor (BDNF) plays an important role in neuronal 
      survival, neurite outgrowth and synaptic plasticity by activating the receptor 
      tropomyosin receptor kinase B (TrkB, also known as NTRK2). TrkB has been shown to 
      undergo recycling after BDNF stimulation. We have previously reported that 
      full-length TrkB (TrkB-FL) are recycled through a Rab11-dependent pathway upon 
      BDNF stimuli, which is important for the translocation of TrkB-FL into dendritic 
      spines and for the maintenance of prolonged BDNF downstream signaling during 
      long-term potentiation (LTP). However, the identity of the motor protein that 
      mediates the local transfer of recycled TrkB-FL back to the plasma membrane 
      remains unclear. Here, we report that the F-actin-based motor protein myosin Va 
      (Myo5a) mediates the postendocytic recycling of TrkB-FL. Blocking the interaction 
      between Rab11 and Myo5a by use of a TAT-tagged peptide consisting of amino acids 
      55-66 of the Myo5a ExonE domain weakened the association between TrkB-FL and 
      Myo5a and thus impaired TrkB-FL recycling and BDNF-induced TrkB-FL translocation 
      into dendritic spines. Finally, inhibiting Myo5a-mediated TrkB-FL recycling led 
      to a significant reduction in prolonged BDNF downstream signaling. Taken 
      together, these results show that Myo5a mediates BDNF-dependent TrkB-FL recycling 
      and contributes to BDNF-induced TrkB spine translocation and prolonged downstream 
      signaling.
CI  - (c) 2015. Published by The Company of Biologists Ltd.
FAU - Sui, Wen-Hai
AU  - Sui WH
AD  - Department of Neurobiology, Shandong Provincial Key Laboratory of Mental 
      Disorders, CAS Center for Excellence in Brain Science, School of Medicine, 
      Shandong University, No.44 Wenhua Xi Road, Jinan, Shandong 250012, P.R. China.
FAU - Huang, Shu-Hong
AU  - Huang SH
AD  - Department of Neurobiology, Shandong Provincial Key Laboratory of Mental 
      Disorders, CAS Center for Excellence in Brain Science, School of Medicine, 
      Shandong University, No.44 Wenhua Xi Road, Jinan, Shandong 250012, P.R. China.
FAU - Wang, Jue
AU  - Wang J
AD  - Central Research Laboratory, The Second Hospital of Shandong University, No.247 
      Beiyuan Dajie, Jinan, Shandong 250033, P.R. China.
FAU - Chen, Qun
AU  - Chen Q
AD  - Department of Neurobiology, Shandong Provincial Key Laboratory of Mental 
      Disorders, CAS Center for Excellence in Brain Science, School of Medicine, 
      Shandong University, No.44 Wenhua Xi Road, Jinan, Shandong 250012, P.R. China.
FAU - Liu, Ting
AU  - Liu T
AD  - Department of Neurobiology, Shandong Provincial Key Laboratory of Mental 
      Disorders, CAS Center for Excellence in Brain Science, School of Medicine, 
      Shandong University, No.44 Wenhua Xi Road, Jinan, Shandong 250012, P.R. China.
FAU - Chen, Zhe-Yu
AU  - Chen ZY
AD  - Department of Neurobiology, Shandong Provincial Key Laboratory of Mental 
      Disorders, CAS Center for Excellence in Brain Science, School of Medicine, 
      Shandong University, No.44 Wenhua Xi Road, Jinan, Shandong 250012, P.R. China 
      zheyuchen@sdu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150127
PL  - England
TA  - J Cell Sci
JT  - Journal of cell science
JID - 0052457
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Myo5a protein, mouse)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 3.6.1.- (Myosin Type V)
RN  - EC 3.6.4.1 (Myosin Heavy Chains)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Cells, Cultured
MH  - Dendritic Spines/*metabolism
MH  - Endocytosis/*physiology
MH  - Hippocampus/cytology/*metabolism
MH  - Long-Term Potentiation
MH  - Mass Spectrometry
MH  - Mice
MH  - Myosin Heavy Chains/genetics/*metabolism
MH  - Myosin Type V/genetics/*metabolism
MH  - Neuronal Plasticity
MH  - Neurons/cytology/*metabolism
MH  - Protein Transport
MH  - Rats
MH  - Receptor, trkB/genetics/*metabolism
MH  - Signal Transduction
OTO - NOTNLM
OT  - BDNF
OT  - Myo5a
OT  - Myosin Va
OT  - NTRK2
OT  - Rab11
OT  - Recycling
OT  - TrkB
EDAT- 2015/01/30 06:00
MHDA- 2016/03/11 06:00
CRDT- 2015/01/30 06:00
PHST- 2015/01/30 06:00 [entrez]
PHST- 2015/01/30 06:00 [pubmed]
PHST- 2016/03/11 06:00 [medline]
AID - jcs.160259 [pii]
AID - 10.1242/jcs.160259 [doi]
PST - ppublish
SO  - J Cell Sci. 2015 Mar 15;128(6):1108-22. doi: 10.1242/jcs.160259. Epub 2015 Jan 
      27.