PMID- 25634019
OWN - NLM
STAT- MEDLINE
DCOM- 20160222
LR  - 20221207
IS  - 1432-0614 (Electronic)
IS  - 0175-7598 (Linking)
VI  - 99
IP  - 12
DP  - 2015 Jun
TI  - Characterization of the bacterial community involved in the bioflocculation 
      process of wastewater organic matter in high-loaded MBRs.
PG  - 5327-37
LID - 10.1007/s00253-015-6402-y [doi]
AB  - High-loaded membrane bioreactors (HL-MBRs), i.e., bioreactors equipped with a 
      membrane for biomass retention and operated at extremely short sludge and 
      hydraulic retention times, can concentrate sewage organic matter to facilitate 
      subsequent energy and chemical recovery from these organics. Bioflocculation, 
      accomplished by microorganisms that produce extracellular polymers, is a very 
      important mechanism in these reactors. Bacterial diversity of the sludge and 
      supernatant fraction of HL-MBRs operated at very short sludge retention times 
      (0.125, 0.5, and 1 day) were determined using a PCR-denaturing gradient gel 
      electrophoresis (DGGE) and clone library approach and compared to the diversity 
      in sewage. Already at a sludge retention time (SRT) of 0.125 day, a distinct 
      bacterial community developed compared to the community in sewage. 
      Bioflocculation, however, was low and the majority of the bacteria, especially 
      Arcobacter, were present in the supernatant fraction. Upon increasing SRT from 
      0.125 to 1 day, a much stronger bioflocculation was accompanied by an increased 
      abundance of Bacteroidetes in the (solid) sludge fraction: 27.5 % at an SRT of 
      0.5 day and 46.4 % at an SRT of 1 day. Furthermore, cluster analysis of DGGE 
      profiles revealed that the bacterial community structure in the sludge was 
      different from that in the supernatant. To localize specific bacterial classes in 
      the sludge flocs, fluorescence in situ hybridization (FISH) was carried out with 
      three different bacterial probes. This showed that Betaproteobacteria formed 
      clusters in the sludge flocs whereas Alphaproteobacteria and Gammaproteobacteria 
      were mainly present as single cells.
FAU - Faust, L
AU  - Faust L
AD  - Wetsus-Centre of Excellence for Sustainable Water Technology, P.O. Box 1113, 8900 
      CC, Leeuwarden, The Netherlands, lena.faust1@gmail.com.
FAU - Szendy, M
AU  - Szendy M
FAU - Plugge, C M
AU  - Plugge CM
FAU - van den Brink, P F H
AU  - van den Brink PF
FAU - Temmink, H
AU  - Temmink H
FAU - Rijnaarts, H H M
AU  - Rijnaarts HH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150130
PL  - Germany
TA  - Appl Microbiol Biotechnol
JT  - Applied microbiology and biotechnology
JID - 8406612
RN  - 0 (Sewage)
RN  - 0 (Waste Water)
SB  - IM
MH  - Bacteria/classification/genetics/*isolation & purification/metabolism
MH  - Biodiversity
MH  - Bioreactors/*microbiology
MH  - Flocculation
MH  - Sewage/chemistry/microbiology
MH  - Wastewater/*chemistry/microbiology
EDAT- 2015/01/31 06:00
MHDA- 2016/02/24 06:00
CRDT- 2015/01/31 06:00
PHST- 2014/11/15 00:00 [received]
PHST- 2015/01/10 00:00 [accepted]
PHST- 2015/01/08 00:00 [revised]
PHST- 2015/01/31 06:00 [entrez]
PHST- 2015/01/31 06:00 [pubmed]
PHST- 2016/02/24 06:00 [medline]
AID - 10.1007/s00253-015-6402-y [doi]
PST - ppublish
SO  - Appl Microbiol Biotechnol. 2015 Jun;99(12):5327-37. doi: 
      10.1007/s00253-015-6402-y. Epub 2015 Jan 30.