PMID- 25634304
OWN - NLM
STAT- MEDLINE
DCOM- 20170118
LR  - 20181202
IS  - 1559-0283 (Electronic)
IS  - 1085-9195 (Linking)
VI  - 72
IP  - 2
DP  - 2015 Jun
TI  - Donor Age and Cell Passage Affect Osteogenic Ability of Rat Bone Marrow 
      Mesenchymal Stem Cells.
PG  - 543-9
LID - 10.1007/s12013-014-0500-9 [doi]
AB  - Tissue engineering allows the restoration of pathologically damaged tissues such 
      as cartilage and bone using bio-scaffolds containing functionally active cells. 
      Bone-marrow-derived mesenchymal stem cells (BMSCs) are a promising source of 
      cells for tissue engineering due to their multilineage differentiation potential. 
      However, proliferative and osteogenic abilities of BMSCs, and quantity of stem 
      cells decreases in the bone marrow in aged population. We cultured BMSCs isolated 
      from rats of various ages and evaluated their morphology, activity, and 
      differentiation potential. Cultured BMSCs formed monolayer of fibroblast-like 
      cells and maintained their characteristic morphology for 7-10 generations. Flow 
      cytometry showed that aging of the cultured cell population correlated with the 
      decrease in the expression of mesenchymal and hematopoietic surface markers, such 
      as CD44, CD45, CD90, and CD29. We detected strong correlation between the age of 
      BMSC donor and ALP activity in BMSC culture induced with low doses of 
      dexamethasone and vitamin C. Cells from 2- and 6-week-old donor SD rats exhibited 
      markedly increased ALP activity that coincided with increased bone content and 
      strong positive staining of mineralized nodules. In contrast, BMSCs isolated from 
      10-month-old donors showed the lowest ALP activity, and decreased bone content 
      and mineralized nodules formation. Our results demonstrate that the increase in 
      donor age negatively affects proliferation and differentiation capacity of BMSCs 
      in culture.
FAU - Li, Chao
AU  - Li C
AD  - Department of Orthopaedics, The 2nd Affiliated Hospital of Harbin Medical 
      University, Harbin, 150001, China.
FAU - Wei, Guojun
AU  - Wei G
AD  - Department of Orthopaedics, The 2nd Affiliated Hospital of Harbin Medical 
      University, Harbin, 150001, China.
FAU - Gu, Qun
AU  - Gu Q
AD  - Department of Orthopaedics, The 2nd Affiliated Hospital of Harbin Medical 
      University, Harbin, 150001, China.
FAU - Wen, Gang
AU  - Wen G
AD  - Department of Orthopaedics, The 2nd Affiliated Hospital of Harbin Medical 
      University, Harbin, 150001, China.
FAU - Qi, Baochang
AU  - Qi B
AD  - Department of Orthopaedics, The 2nd Affiliated Hospital of Harbin Medical 
      University, Harbin, 150001, China.
FAU - Xu, Liang
AU  - Xu L
AD  - Department of Orthopaedics, The 2nd Affiliated Hospital of Harbin Medical 
      University, Harbin, 150001, China.
FAU - Tao, Shuqin
AU  - Tao S
AD  - Department of Orthopaedics, The 2nd Affiliated Hospital of Harbin Medical 
      University, Harbin, 150001, China. drshuqintao@163.com.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cell Biochem Biophys
JT  - Cell biochemistry and biophysics
JID - 9701934
SB  - IM
MH  - Animals
MH  - Bone Marrow/*growth & development
MH  - *Cell Differentiation
MH  - Cell Lineage
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Female
MH  - Mesenchymal Stem Cells/*cytology/physiology
MH  - *Osteogenesis
MH  - Rats
MH  - Rats, Sprague-Dawley
OTO - NOTNLM
OT  - Ages
OT  - Bone marrow
OT  - Mesenchymal stem cells
OT  - Osteogenesis
EDAT- 2015/01/31 06:00
MHDA- 2017/01/19 06:00
CRDT- 2015/01/31 06:00
PHST- 2015/01/31 06:00 [entrez]
PHST- 2015/01/31 06:00 [pubmed]
PHST- 2017/01/19 06:00 [medline]
AID - 10.1007/s12013-014-0500-9 [pii]
AID - 10.1007/s12013-014-0500-9 [doi]
PST - ppublish
SO  - Cell Biochem Biophys. 2015 Jun;72(2):543-9. doi: 10.1007/s12013-014-0500-9.