PMID- 25635270
OWN - NLM
STAT- MEDLINE
DCOM- 20160314
LR  - 20200826
IS  - 1465-2080 (Electronic)
IS  - 1350-0872 (Linking)
VI  - 161
IP  - Pt 4
DP  - 2015 Apr
TI  - Effect of iron on the probiotic properties of the vaginal isolate Lactobacillus 
      jensenii CECT 4306.
PG  - 708-18
LID - 10.1099/mic.0.000044 [doi]
AB  - The vaginal microbiota of healthy, fertile women is dominated by lactobacilli. As 
      a defence mechanism, these bacteria produce H(2)O(2) to discourage colonization of 
      the vagina by undesirable micro-organisms. In particular, Lactobacillus jensenii 
      CECT 4306 is a strong producer of H(2)O(2) and has been found to protect itself from 
      the bactericidal effects of this compound through the activity of extracellular 
      peroxidases. However, this peroxidase activity is dependent on the presence of 
      Fe(3+), which is found in elevated concentrations in the vaginal mucosa as a 
      consequence of the menstrual discharge. The aim of the present work was to 
      evaluate whether Fe(3+) is able to modulate other potential probiotic properties 
      of strain 4306. We found that Fe(3+) enhances the adhesion of L. jensenii CECT 
      4306 to mucin and to HT-29 and HT-29 MTX cells, and, in addition, improves the 
      anti-inflammatory profile, as judged by an increase in the ratio of 
      IL-10/IL-12p70 that were secreted by macrophages. A comparison of total, secreted 
      and surface proteins produced in the presence and absence of Fe(3+) revealed 
      significant differences in the concentration of the moonlighting protein 
      glyceraldehyde-3-phosphate dehydrogenase (GAPDH). In conclusion, Fe(3+) seems to 
      improve the probiotic characteristics of L. jensenii CECT 4306, and future 
      research of the interactions of this strain with its vaginal environment may 
      reveal further information about different aspects of its probiotic potential.
CI  - (c) 2015 The Authors.
FAU - Martin, Rebeca
AU  - Martin R
AD  - INRA, UMR1319 Micalis, F-78350 Jouy-en-Josas, France AgroParisTech, UMR Micalis, 
      F-78350 Jouy-en-Josas, France Area de Microbiologia, Universidad de Oviedo, 
      Julian Claveria SN, 33006, Oviedo, Spain.
FAU - Sanchez, Borja
AU  - Sanchez B
AD  - Department of Analytical and Food Chemistry, Faculty of Food Science and 
      Technology, University of Vigo, 32004, Ourense, Spain.
FAU - Urdaci, Maria C
AU  - Urdaci MC
AD  - Universite de Bordeaux, UMR 5248, Bordeaux Sciences Agro, F-33175 Gradignan, 
      France.
FAU - Langella, Philippe
AU  - Langella P
AD  - INRA, UMR1319 Micalis, F-78350 Jouy-en-Josas, France AgroParisTech, UMR Micalis, 
      F-78350 Jouy-en-Josas, France.
FAU - Suarez, Juan E
AU  - Suarez JE
AD  - Area de Microbiologia, Universidad de Oviedo, Julian Claveria SN, 33006, Oviedo, 
      Spain IPLA-CSIC, Carretera de Infiesto SN, 33300, Villaviciosa, Spain.
FAU - Bermudez-Humaran, Luis G
AU  - Bermudez-Humaran LG
AD  - INRA, UMR1319 Micalis, F-78350 Jouy-en-Josas, France AgroParisTech, UMR Micalis, 
      F-78350 Jouy-en-Josas, France luis.bermudez@jouy.inra.fr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150129
PL  - England
TA  - Microbiology (Reading)
JT  - Microbiology (Reading, England)
JID - 9430468
RN  - 0 (Bacterial Proteins)
RN  - 0 (Ferric Compounds)
RN  - 0 (Proteome)
RN  - E1UOL152H7 (Iron)
SB  - IM
MH  - Bacterial Adhesion
MH  - Bacterial Proteins
MH  - Cell Line
MH  - Female
MH  - Ferric Compounds/metabolism
MH  - Humans
MH  - Immunomodulation
MH  - Iron/*metabolism
MH  - Lactobacillus/*metabolism
MH  - Microbiota
MH  - Mucous Membrane/immunology/microbiology
MH  - *Probiotics
MH  - Proteome
MH  - Proteomics
MH  - Vagina/immunology/*microbiology
EDAT- 2015/01/31 06:00
MHDA- 2016/03/15 06:00
CRDT- 2015/01/31 06:00
PHST- 2014/12/05 00:00 [received]
PHST- 2015/01/22 00:00 [accepted]
PHST- 2015/01/31 06:00 [entrez]
PHST- 2015/01/31 06:00 [pubmed]
PHST- 2016/03/15 06:00 [medline]
AID - mic.0.000044 [pii]
AID - 10.1099/mic.0.000044 [doi]
PST - ppublish
SO  - Microbiology (Reading). 2015 Apr;161(Pt 4):708-18. doi: 10.1099/mic.0.000044. 
      Epub 2015 Jan 29.