PMID- 25635980
OWN - NLM
STAT- MEDLINE
DCOM- 20150604
LR  - 20220330
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 19
IP  - 1
DP  - 2015 Jan
TI  - Wnt3a promotes human umbilical cord mesenchymal stem cells to differentiate into 
      epidermal-like cells.
PG  - 86-91
LID - 8339 [pii]
AB  - OBJECTIVE: Mesenchymal stem cells are a population of pluripotent cells that can 
      differentiate into epidermal-like cells under certain conditions. Wnt3a can 
      promote the proliferation and differentiation of stem cells. However, the role of 
      Wnt3a in the differentiation of human umbilical cord mesenchymal stem cells 
      (hUCMSCs) into epidermal-like cells is unknown. MATERIALS AND METHODS: 
      Third-generation hUCMSCs were cultured in normal medium, epidermal stem 
      cell-conditioned medium, and conditioned medium with added Wnt3a. After culturing 
      for 5 days, the expression of cytokeratin 19 (CK19), an antigen specific for 
      epidermal-like cells, was assessed by immunofluorescence and flow cytometry. The 
      expression of CK19 mRNA was confirmed by reverse transcription-polymerase chain 
      reaction(RT-PCR), and beta-catenin expression was detected by western blot. RESULTS: 
      hUCMSCs differentiated into epidermal-like cells when cultured in conditioned 
      medium as shown by positive immunofluorescence staining for CK19. Flow cytometry 
      showed that the number of cells positive for CK19 in the epidermal stem 
      cell-conditioned medium group was significantly higher than that of control 
      group, but lower than that of the Wnt3a-conditioned group (p < 0.05). RT-PCR 
      showed that the expression level of CK19 mRNA in the conditioned medium group was 
      significantly lower than that of the Wnt3a group (p < 0.01). Westernblots showed 
      that the expression of beta-catenin in the conditioned medium group was 
      significantly lower than that of the Wnt3a group (p < 0.01). CONCLUSIONS: These 
      results suggest that Wnt3a can effectively promote the differentiation of hUCMSCs 
      into epidermal-like cells.
FAU - Sun, T-J
AU  - Sun TJ
AD  - Department of Burn and Plastic Surgery, Hainan Branch of People's Liberation Army 
      General Hospital, Sanya, People's Republic of China. dochyf@163.com.
FAU - Tao, R
AU  - Tao R
FAU - Han, Y-Q
AU  - Han YQ
FAU - Xu, G
AU  - Xu G
FAU - Liu, J
AU  - Liu J
FAU - Han, Y-F
AU  - Han YF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (WNT3A protein, human)
RN  - 0 (Wnt3A Protein)
SB  - IM
MH  - Cell Differentiation/drug effects
MH  - Cells, Cultured
MH  - Culture Media, Conditioned
MH  - *Epidermal Cells
MH  - Flow Cytometry
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology/*drug effects
MH  - Umbilical Cord/*cytology/*drug effects
MH  - Wnt3A Protein/*pharmacology
EDAT- 2015/01/31 06:00
MHDA- 2015/06/05 06:00
CRDT- 2015/01/31 06:00
PHST- 2015/01/31 06:00 [entrez]
PHST- 2015/01/31 06:00 [pubmed]
PHST- 2015/06/05 06:00 [medline]
AID - 8339 [pii]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2015 Jan;19(1):86-91.