PMID- 25636275
OWN - NLM
STAT- MEDLINE
DCOM- 20150910
LR  - 20181202
IS  - 1006-7248 (Print)
IS  - 1006-7248 (Linking)
VI  - 23
IP  - 6
DP  - 2014 Dec
TI  - [Effect of high glucose on migration of BMSCs through inhibiting CXCR-4].
PG  - 646-50
AB  - PURPOSE: To explore the effect of high glucose on migration of BMSCs through 
      inhibiting CXCR-4. METHODS: Bone marrow stromal cells (BMSCs) were obtained from 
      the mandible of Wistar rats and stimulated with different concentrations of 
      glucose (5.5, 16.5 mmol/L). The optimum concentration of SDF-1 was evaluated by 
      Transwell assay in physiological glucose concentration (5.5 mmol/L). In the 
      optimum concentration of SDF-1 condition, we detected the effect of SDF-1 and 
      AMD3100 on migration of BMSCs in different concentrations of glucose (5.5, 16.5 
      mmol/L). CXCR-4 protein levels were determined by Western blot. The mRNA 
      expression of CXCR-4 and MMP-2 were tested by RT-PCR. SPSS 11.0 software package 
      was used for statistical analysis. RESULTS: The optimum concentration of SDF-1 
      was 100 ng/mL. High glucose could inhibit the migration of BMSCs. In different 
      concentrations of glucose, SDF-1 could promote the migration of BMSCs, but 
      AMD3100 could inhibit this promotion. High glucose condition could inhibit the 
      secretion of CXCR-4 and mRNA expression of CXCR-4 and MMP-2. CONCLUSIONS: High 
      glucose inhibits migration of BMSCs by inhibiting CXCR-4 through SDF-1/CXCR-4 
      pathway.
FAU - Zhang, Bo
AU  - Zhang B
AD  - Stomatological Center, General Hospital of PLA. Beijing 100853, 
      China.E-mail:hugo_zhang_005@163.com.
FAU - Liu, Na
AU  - Liu N
FAU - Gu, Bin
AU  - Gu B
FAU - Wu, Hao
AU  - Wu H
FAU - Gao, Yu-xuan
AU  - Gao YX
FAU - Wang, Dong-sheng
AU  - Wang DS
FAU - Liu, Hong-chen
AU  - Liu HC
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Shanghai Kou Qiang Yi Xue
JT  - Shanghai kou qiang yi xue = Shanghai journal of stomatology
JID - 101090220
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Receptors, CXCR4)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - *Cell Movement
MH  - Chemokine CXCL12
MH  - Glucose
MH  - *Mesenchymal Stem Cells
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, CXCR4
EDAT- 2015/02/01 06:00
MHDA- 2015/09/12 06:00
CRDT- 2015/02/01 06:00
PHST- 2015/02/01 06:00 [entrez]
PHST- 2015/02/01 06:00 [pubmed]
PHST- 2015/09/12 06:00 [medline]
PST - ppublish
SO  - Shanghai Kou Qiang Yi Xue. 2014 Dec;23(6):646-50.