PMID- 25637306
OWN - NLM
STAT- MEDLINE
DCOM- 20151228
LR  - 20150414
IS  - 1096-0309 (Electronic)
IS  - 0003-2697 (Linking)
VI  - 475
DP  - 2015 Apr 15
TI  - "Turn off-on" phosphorescent biosensors for detection of DNA based on quantum 
      dots/acridine orange.
PG  - 32-9
LID - S0003-2697(15)00024-X [pii]
LID - 10.1016/j.ab.2015.01.011 [doi]
AB  - A "turn off-on" switch mode was established by using the interaction between 
      acridine orange (AO) and DNA as an input signal and using the room temperature 
      phosphorescence (RTP) reversible change of 3-mercaptopropionic acid (MPA)-capped 
      Mn-doped ZnS quantum dots (QDs) as an output signal in biological fluids. AO was 
      absorbed into the surface of Mn-doped ZnS QDs via electrostatic attraction and, 
      thus, formed a ground-state complex through photoinduced electron transfer 
      (PIET). This complex quenched the phosphorescence of Mn-doped ZnS QDs and then 
      rendered the system into the "turn-off" mode. Along with the addition of DNA and 
      embedded binding with DNA, AO was competitively induced to fall off from the 
      surface of Mn-doped ZnS QDs and embed into the double helix structure of DNA. As 
      a result, the RTP of Mn-doped ZnS QDs was recovered and the system consequently 
      was rendered into "turn-on" mode. In this case, a new biosensor for DNA detection 
      was built and has a detection limit of 0.033mgL(-1) and a detection range from 
      0.033 to 20mgL(-1). What is more, this kind of biosensor does not require complex 
      pretreatments and is free from the interference from autofluorescence and 
      scattering light. Thus, this biosensor can be used to detect DNA in biological 
      fluids.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Miao, Yanming
AU  - Miao Y
AD  - Shanxi Normal University, Linfen 041004, People's Republic of China. Electronic 
      address: mym8207@126.com.
FAU - Li, Yuting
AU  - Li Y
AD  - Shanxi Normal University, Linfen 041004, People's Republic of China.
FAU - Zhang, Zhifeng
AU  - Zhang Z
AD  - Shanxi Normal University, Linfen 041004, People's Republic of China.
FAU - Yan, Guiqin
AU  - Yan G
AD  - Shanxi Normal University, Linfen 041004, People's Republic of China.
FAU - Bi, Yi
AU  - Bi Y
AD  - Yantai University, Yantai 264005, People's Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150127
PL  - United States
TA  - Anal Biochem
JT  - Analytical biochemistry
JID - 0370535
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Molecular Probes)
RN  - 9007-49-2 (DNA)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/*chemistry
MH  - Biosensing Techniques/*methods
MH  - DNA/*chemistry
MH  - Fluorescent Dyes/*chemistry
MH  - Molecular Probes/*chemistry
MH  - Quantum Dots/*chemistry
OTO - NOTNLM
OT  - Acridine orange
OT  - Biological fluids
OT  - DNA
OT  - Turn off-on
EDAT- 2015/02/01 06:00
MHDA- 2015/12/29 06:00
CRDT- 2015/02/01 06:00
PHST- 2014/11/23 00:00 [received]
PHST- 2015/01/12 00:00 [revised]
PHST- 2015/01/15 00:00 [accepted]
PHST- 2015/02/01 06:00 [entrez]
PHST- 2015/02/01 06:00 [pubmed]
PHST- 2015/12/29 06:00 [medline]
AID - S0003-2697(15)00024-X [pii]
AID - 10.1016/j.ab.2015.01.011 [doi]
PST - ppublish
SO  - Anal Biochem. 2015 Apr 15;475:32-9. doi: 10.1016/j.ab.2015.01.011. Epub 2015 Jan 
      27.