PMID- 25637353
OWN - NLM
STAT- MEDLINE
DCOM- 20150511
LR  - 20181113
IS  - 1460-2075 (Electronic)
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 34
IP  - 6
DP  - 2015 Mar 12
TI  - Space exploration by dendritic cells requires maintenance of myosin II activity 
      by IP3 receptor 1.
PG  - 798-810
LID - 10.15252/embj.201489056 [doi]
AB  - Dendritic cells (DCs) patrol the interstitial space of peripheral tissues. The 
      mechanisms that regulate their migration in such constrained environment remain 
      unknown. We here investigated the role of calcium in immature DCs migrating in 
      confinement. We found that they displayed calcium oscillations that were 
      independent of extracellular calcium and more frequently observed in DCs 
      undergoing strong speed fluctuations. In these cells, calcium spikes were 
      associated with fast motility phases. IP(3) receptors (IP(3)Rs) channels, which allow 
      calcium release from the endoplasmic reticulum, were identified as required for 
      immature DCs to migrate at fast speed. The IP(3)R1 isoform was further shown to 
      specifically regulate the locomotion persistence of immature DCs, that is, their 
      capacity to maintain directional migration. This function of IP(3)R1 results from 
      its ability to control the phosphorylation levels of myosin II regulatory light 
      chain (MLC) and the back/front polarization of the motor protein. We propose that 
      by upholding myosin II activity, constitutive calcium release from the ER through 
      IP(3)R1 maintains DC polarity during migration in confinement, facilitating the 
      exploration of their environment.
CI  - (c) 2015 Institut Curie/Inserm. Published under the terms of the CC BY NC ND 4.0 
      license.
FAU - Solanes, Paola
AU  - Solanes P
AD  - Inserm-U932 Institut Curie, Paris, France.
FAU - Heuze, Melina L
AU  - Heuze ML
AD  - Inserm-U932 Institut Curie, Paris, France.
FAU - Maurin, Mathieu
AU  - Maurin M
AD  - Inserm-U932 Institut Curie, Paris, France.
FAU - Bretou, Marine
AU  - Bretou M
AD  - Inserm-U932 Institut Curie, Paris, France.
FAU - Lautenschlaeger, Franziska
AU  - Lautenschlaeger F
AD  - CNRS-UMR144 Institut Curie, Paris, France.
FAU - Maiuri, Paolo
AU  - Maiuri P
AD  - CNRS-UMR144 Institut Curie, Paris, France.
FAU - Terriac, Emmanuel
AU  - Terriac E
AD  - CNRS-UMR144 Institut Curie, Paris, France.
FAU - Thoulouze, Maria-Isabel
AU  - Thoulouze MI
AD  - Unite de Virologie Structurale, CNRS-UMR3469 Institut Pasteur, Paris, France.
FAU - Launay, Pierre
AU  - Launay P
AD  - Faculte de Medecine X. Bichat, Inserm-U1149, Paris, France.
FAU - Piel, Matthieu
AU  - Piel M
AD  - CNRS-UMR144 Institut Curie, Paris, France.
FAU - Vargas, Pablo
AU  - Vargas P
AD  - Inserm-U932 Institut Curie, Paris, France pablo.vargas@curie.fr 
      amlennon@curie.fr.
FAU - Lennon-Dumenil, Ana-Maria
AU  - Lennon-Dumenil AM
AD  - Inserm-U932 Institut Curie, Paris, France pablo.vargas@curie.fr 
      amlennon@curie.fr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150130
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (DNA Primers)
RN  - 0 (Inositol 1,4,5-Trisphosphate Receptors)
RN  - EC 3.6.1.- (Myosin Type II)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/*metabolism
MH  - Cell Movement/*physiology
MH  - Cell Polarity
MH  - DNA Primers/genetics
MH  - Dendritic Cells/*immunology
MH  - Endoplasmic Reticulum/metabolism
MH  - Extracellular Space/*immunology
MH  - Flow Cytometry
MH  - Immunoblotting
MH  - Inositol 1,4,5-Trisphosphate Receptors/*metabolism
MH  - Mice
MH  - Microscopy, Fluorescence
MH  - Microscopy, Video
MH  - Myosin Type II/*metabolism
MH  - Phosphorylation
MH  - Real-Time Polymerase Chain Reaction
PMC - PMC4369315
OTO - NOTNLM
OT  - IP3 receptors
OT  - calcium
OT  - cell migration
OT  - dendritic cells
OT  - myosin II
EDAT- 2015/02/01 06:00
MHDA- 2015/05/12 06:00
PMCR- 2016/03/12
CRDT- 2015/02/01 06:00
PHST- 2015/02/01 06:00 [entrez]
PHST- 2015/02/01 06:00 [pubmed]
PHST- 2015/05/12 06:00 [medline]
PHST- 2016/03/12 00:00 [pmc-release]
AID - embj.201489056 [pii]
AID - 10.15252/embj.201489056 [doi]
PST - ppublish
SO  - EMBO J. 2015 Mar 12;34(6):798-810. doi: 10.15252/embj.201489056. Epub 2015 Jan 
      30.