PMID- 25637754
OWN - NLM
STAT- MEDLINE
DCOM- 20150413
LR  - 20150223
IS  - 1879-3169 (Electronic)
IS  - 0378-4274 (Linking)
VI  - 233
IP  - 2
DP  - 2015 Mar 4
TI  - Influence of in utero di-n-hexyl phthalate and dicyclohexyl phthalate on fetal 
      testicular development in rats.
PG  - 125-37
LID - S0378-4274(15)00027-2 [pii]
LID - 10.1016/j.toxlet.2015.01.015 [doi]
AB  - This study investigated the effects of di-n-hexyl phthalate (DHP) and 
      dicyclohexyl phthalate (DCHP) on male reproductive development in utero. Pregnant 
      rats were exposed to DHP and DCHP at doses of 0 (vehicle), 20, 100 and 
      500mg/kg/day, by gavage, on gestational days (GD) 6-19. A significant decrease in 
      the anogenital distance (AGD) of male fetuses was observed at all doses of DHP 
      and DCHP. The AGD/cube root of body weight ratio in male fetuses was also 
      significantly reduced compared to control group. The litters with resorption, 
      percentage of resorptions and inhibin B levels increased in treatment groups. 
      Moreover, testosterone and MIS/AMH levels in all treatment groups decreased. 
      Although FSH and inhibin B levels of male pups exposed to DHP and DCHP increased, 
      FSH/inhibin B ratio decreased in treatment groups. Reduced testosterone 
      production in response to DHP and DCHP exposure appeared to be related to changes 
      in testosterone metabolism, as shown by decreased 3beta-HSD immunoexpression. The 
      percentages of large Leydig clusters increased after exposure to DHP and DCHP in 
      utero. Histopathological examination of the testis on GD20 revealed changes at 
      all doses. Relative integrated immunodensities of 3beta-HSD, MIS/AMH, PCNA and AR 
      decreased after DHP and DCHP exposures. Altered fetal Sertoli cell development 
      and function may be caused by disrupted PMC function revealed by reduced AR 
      production in these cells in treatment groups.
CI  - Copyright (c) 2015 Elsevier Ireland Ltd. All rights reserved.
FAU - Aydogan Ahbab, Mufide
AU  - Aydogan Ahbab M
AD  - Department of Biology, Faculty of Science, University of Hacettepe, Ankara, 
      Turkey. Electronic address: mufideaydogan@gmail.com.
FAU - Barlas, Nurhayat
AU  - Barlas N
AD  - Department of Biology, Faculty of Science, University of Hacettepe, Ankara, 
      Turkey.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150128
PL  - Netherlands
TA  - Toxicol Lett
JT  - Toxicology letters
JID - 7709027
RN  - 0 (Gonadal Steroid Hormones)
RN  - 0 (Phthalic Acids)
RN  - 0 (Teratogens)
RN  - 3XMK78S47O (Testosterone)
RN  - 42MAH1QFG5 (di-n-hexyl phthalate)
RN  - CGD15M7H2N (dicyclohexyl phthalate)
SB  - IM
MH  - Animals
MH  - Body Weight/drug effects
MH  - Drinking/drug effects
MH  - Eating/drug effects
MH  - Female
MH  - Gonadal Steroid Hormones/blood
MH  - Leydig Cells/drug effects
MH  - Male
MH  - Phthalic Acids/*toxicity
MH  - Pregnancy
MH  - Rats
MH  - Rats, Wistar
MH  - Teratogens/*toxicity
MH  - Testis/drug effects/*embryology/metabolism
MH  - Testosterone/metabolism
OTO - NOTNLM
OT  - Aantiandrogen
OT  - Di-n-hexyl phthalate
OT  - Dicyclohexyl phthalate
OT  - Endocrine disrupters
OT  - In utero
OT  - Testicular development
EDAT- 2015/02/01 06:00
MHDA- 2015/04/14 06:00
CRDT- 2015/02/01 06:00
PHST- 2014/03/27 00:00 [received]
PHST- 2015/01/23 00:00 [revised]
PHST- 2015/01/27 00:00 [accepted]
PHST- 2015/02/01 06:00 [entrez]
PHST- 2015/02/01 06:00 [pubmed]
PHST- 2015/04/14 06:00 [medline]
AID - S0378-4274(15)00027-2 [pii]
AID - 10.1016/j.toxlet.2015.01.015 [doi]
PST - ppublish
SO  - Toxicol Lett. 2015 Mar 4;233(2):125-37. doi: 10.1016/j.toxlet.2015.01.015. Epub 
      2015 Jan 28.