PMID- 25637783
OWN - NLM
STAT- MEDLINE
DCOM- 20150519
LR  - 20150327
IS  - 1090-2449 (Electronic)
IS  - 0014-4894 (Linking)
VI  - 150
DP  - 2015 Mar
TI  - Cryptosporidium huwi n. sp. (Apicomplexa: Eimeriidae) from the guppy (Poecilia 
      reticulata).
PG  - 31-5
LID - S0014-4894(15)00017-X [pii]
LID - 10.1016/j.exppara.2015.01.009 [doi]
AB  - The morphological, biological, and molecular characteristics of Cryptosporidium 
      piscine genotype 1 from the guppy (Poecilia reticulata) are described, and the 
      species name Cryptosporidium huwi n. sp. is proposed to reflect its genetic and 
      biological differences from gastric and intestinal Cryptosporidium species. 
      Oocysts of C.huwi n. sp. over-lap in size with Cryptosporidium molnari, measuring 
      approximately 4.4-4.9 microm (mean 4.6) by 4.0-4.8 microm (mean 4.4 microm) with a length to 
      width ratio of 1.04 (0.92-1.35) (n = 50). Similar to C.molnari, C.huwi n. sp. was 
      identified in the stomach only and clusters of oogonial and sporogonial stages 
      were identified deep within the epithelium. However, phylogenetic analysis of 18S 
      rRNA sequences indicated that C. huwi n. sp. exhibited 8.5-9.2% and 3.5% genetic 
      distance from C.molnari isolates and piscine genotype 7 respectively. At the 
      actin locus, the genetic distance between C.huwi n. sp. and C.molnari was 16.6%. 
      The genetic distance between C.huwi n. sp. and other Cryptosporidium species at 
      the 18S locus was 13.2%-17% and at the actin locus was 18.9%-26.3%. Therefore C. 
      huwi n. sp. is genetically distinct from previously described Cryptosporidium 
      species.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Ryan, Una
AU  - Ryan U
AD  - School of Veterinary and Life Sciences, Murdoch University, Murdoch, Perth, 
      Western Australia 6150, Australia. Electronic address: una.ryan@murdoch.edu.au.
FAU - Paparini, Andrea
AU  - Paparini A
AD  - School of Veterinary and Life Sciences, Murdoch University, Murdoch, Perth, 
      Western Australia 6150, Australia.
FAU - Tong, Kaising
AU  - Tong K
AD  - School of Veterinary and Life Sciences, Murdoch University, Murdoch, Perth, 
      Western Australia 6150, Australia.
FAU - Yang, Rongchang
AU  - Yang R
AD  - School of Veterinary and Life Sciences, Murdoch University, Murdoch, Perth, 
      Western Australia 6150, Australia.
FAU - Gibson-Kueh, Susan
AU  - Gibson-Kueh S
AD  - School of Veterinary and Life Sciences, Murdoch University, Murdoch, Perth, 
      Western Australia 6150, Australia.
FAU - O'Hara, Amanda
AU  - O'Hara A
AD  - School of Veterinary and Life Sciences, Murdoch University, Murdoch, Perth, 
      Western Australia 6150, Australia.
FAU - Lymbery, Alan
AU  - Lymbery A
AD  - School of Veterinary and Life Sciences, Murdoch University, Murdoch, Perth, 
      Western Australia 6150, Australia.
FAU - Xiao, Lihua
AU  - Xiao L
AD  - Public Health Services, U.S. Department of Health and Human Services, Centers for 
      Disease Control and Prevention, Atlanta, GA, USA.
LA  - eng
PT  - Journal Article
DEP - 20150128
PL  - United States
TA  - Exp Parasitol
JT  - Experimental parasitology
JID - 0370713
RN  - 0 (DNA, Protozoan)
RN  - 0 (RNA, Ribosomal, 18S)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cryptosporidiosis/epidemiology/*parasitology
MH  - Cryptosporidium/*classification/genetics
MH  - DNA, Protozoan/chemistry/isolation & purification
MH  - Fish Diseases/epidemiology/*parasitology
MH  - Genotype
MH  - Phylogeny
MH  - Poecilia/*parasitology
MH  - Polymerase Chain Reaction
MH  - Prevalence
MH  - RNA, Ribosomal, 18S/genetics
MH  - Sequence Alignment/veterinary
MH  - Stomach/parasitology
OTO - NOTNLM
OT  - 18S rRNA
OT  - Actin gene
OT  - Cryptosporidium huwi n. sp
OT  - Genetic characterization
OT  - Morphology
OT  - Phylogeny
EDAT- 2015/02/01 06:00
MHDA- 2015/05/20 06:00
CRDT- 2015/02/01 06:00
PHST- 2014/07/29 00:00 [received]
PHST- 2014/12/08 00:00 [revised]
PHST- 2015/01/22 00:00 [accepted]
PHST- 2015/02/01 06:00 [entrez]
PHST- 2015/02/01 06:00 [pubmed]
PHST- 2015/05/20 06:00 [medline]
AID - S0014-4894(15)00017-X [pii]
AID - 10.1016/j.exppara.2015.01.009 [doi]
PST - ppublish
SO  - Exp Parasitol. 2015 Mar;150:31-5. doi: 10.1016/j.exppara.2015.01.009. Epub 2015 
      Jan 28.