PMID- 25638529
OWN - NLM
STAT- MEDLINE
DCOM- 20151214
LR  - 20181202
IS  - 1879-1298 (Electronic)
IS  - 0045-6535 (Linking)
VI  - 128
DP  - 2015 Jun
TI  - Physiological fluxes and antioxidative enzymes activities of immobilized 
      Phanerochaete chrysosporium loaded with TiO2 nanoparticles after exposure to 
      toxic pollutants in solution.
PG  - 21-7
LID - S0045-6535(15)00032-6 [pii]
LID - 10.1016/j.chemosphere.2014.12.088 [doi]
AB  - Immobilized Phanerochaete chrysosporium loaded with TiO2 nanoparticles (PTNs) are 
      novel high-value bioremediation materials for adsorbing cadmium and for degrading 
      2,4-dichlorophenol (2,4-DCP). The real-time changes in H(+) and O2 fluxes were 
      measured using the noninvasive microtest technique (NMT). The H(+) influx 
      increased after the addition of 2,4-DCP, and shifted to efflux following the 
      addition of Cd(2+). The O2 flux decreased after the addition of both 2,4-DCP and 
      Cd(2+). A larger Cd(2+) flux was immediately observed after exposure to 0.5mM 
      Cd(2+) (-351.25 pmol cm(-2) s(-1)) than to 0.1 mM Cd(2+) (-107.47 pmol cm(-2) 
      s(-1)). The removal of Cd(2+) by the PTNs increased more after treatment with the 
      0.5 mM exposure solution (27.6 mg g(-1)) than with the 0.1 mM exposure solution 
      (3.49 mg g(-1)). The enzyme activities were analyzed to review the antioxidative 
      defense system of PTNs in a solution containing various concentrations of Cd(2+). 
      The activities of the coenzyme nicotinamide adenine dinucleotide (NADH) oxidase 
      as well as the enzyme catalase (CAT) plateaued at 6.5 U g(-1) FW and 9.7 U g(-1) 
      FW, respectively, after exposure to 0.25 mM Cd(2+). The activity of superoxide 
      dismutase (SOD) increased gradually in solutions containing 0.1-0.6 mM Cd(2+), 
      and eventually reached a maximum (68.86 U g(-1) FW). These results illustrate how 
      the antioxidative defense system and the physiological fluxes of PTNs respond to 
      the stress caused by toxic pollutants.
CI  - Copyright (c) 2015 Elsevier Ltd. All rights reserved.
FAU - Tan, Qiong
AU  - Tan Q
AD  - College of Environmental Science and Engineering, Hunan University, Changsha 
      410082, PR China; Key Laboratory of Environmental Biology and Pollution Control 
      (Hunan University), Ministry of Education, Changsha 410082, PR China.
FAU - Chen, Guiqiu
AU  - Chen G
AD  - College of Environmental Science and Engineering, Hunan University, Changsha 
      410082, PR China; Key Laboratory of Environmental Biology and Pollution Control 
      (Hunan University), Ministry of Education, Changsha 410082, PR China. Electronic 
      address: gqchen@hnu.edu.cn.
FAU - Zeng, Guangming
AU  - Zeng G
AD  - College of Environmental Science and Engineering, Hunan University, Changsha 
      410082, PR China; Key Laboratory of Environmental Biology and Pollution Control 
      (Hunan University), Ministry of Education, Changsha 410082, PR China. Electronic 
      address: zgming@hnu.edu.cn.
FAU - Chen, Anwei
AU  - Chen A
AD  - College of Resources and Environment, Hunan Agricultural University, Changsha 
      410128, PR China.
FAU - Guan, Song
AU  - Guan S
AD  - College of Environmental Science and Engineering, Hunan University, Changsha 
      410082, PR China; Key Laboratory of Environmental Biology and Pollution Control 
      (Hunan University), Ministry of Education, Changsha 410082, PR China.
FAU - Li, Zhongwu
AU  - Li Z
AD  - College of Environmental Science and Engineering, Hunan University, Changsha 
      410082, PR China; Key Laboratory of Environmental Biology and Pollution Control 
      (Hunan University), Ministry of Education, Changsha 410082, PR China.
FAU - Zuo, Yanan
AU  - Zuo Y
AD  - College of Environmental Science and Engineering, Hunan University, Changsha 
      410082, PR China; Key Laboratory of Environmental Biology and Pollution Control 
      (Hunan University), Ministry of Education, Changsha 410082, PR China.
FAU - Huang, Zhenzhen
AU  - Huang Z
AD  - College of Environmental Science and Engineering, Hunan University, Changsha 
      410082, PR China; Key Laboratory of Environmental Biology and Pollution Control 
      (Hunan University), Ministry of Education, Changsha 410082, PR China.
FAU - Guo, Zhi
AU  - Guo Z
AD  - College of Environmental Science and Engineering, Hunan University, Changsha 
      410082, PR China; Key Laboratory of Environmental Biology and Pollution Control 
      (Hunan University), Ministry of Education, Changsha 410082, PR China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150129
PL  - England
TA  - Chemosphere
JT  - Chemosphere
JID - 0320657
RN  - 0 (Antioxidants)
RN  - 0 (Chlorophenols)
RN  - 0 (Protons)
RN  - 0 (Water Pollutants, Chemical)
RN  - 00BH33GNGH (Cadmium)
RN  - 15FIX9V2JP (titanium dioxide)
RN  - D1JT611TNE (Titanium)
RN  - R669TG1950 (2,4-dichlorophenol)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Antioxidants/*pharmacology
MH  - Biodegradation, Environmental
MH  - Cadmium/toxicity
MH  - Chlorophenols/toxicity
MH  - Environmental Restoration and Remediation/instrumentation
MH  - Metal Nanoparticles/analysis
MH  - Oxygen/adverse effects
MH  - Phanerochaete/*drug effects/enzymology
MH  - Protons/adverse effects
MH  - Titanium/*pharmacology
MH  - Water Pollutants, Chemical/*toxicity
OTO - NOTNLM
OT  - 2,4-Dichlorophenol
OT  - Cadmium
OT  - Enzymes
OT  - Microbe
OT  - Physiological fluxes
EDAT- 2015/02/02 06:00
MHDA- 2015/12/15 06:00
CRDT- 2015/02/02 06:00
PHST- 2014/08/25 00:00 [received]
PHST- 2014/12/19 00:00 [revised]
PHST- 2014/12/24 00:00 [accepted]
PHST- 2015/02/02 06:00 [entrez]
PHST- 2015/02/02 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
AID - S0045-6535(15)00032-6 [pii]
AID - 10.1016/j.chemosphere.2014.12.088 [doi]
PST - ppublish
SO  - Chemosphere. 2015 Jun;128:21-7. doi: 10.1016/j.chemosphere.2014.12.088. Epub 2015 
      Jan 29.