PMID- 25639817
OWN - NLM
STAT- MEDLINE
DCOM- 20160412
LR  - 20161125
IS  - 1751-553X (Electronic)
IS  - 1751-5521 (Linking)
VI  - 37
IP  - 4
DP  - 2015 Aug
TI  - Utility and impact of early t(15;17) identification by Fluorescence In Situ 
      Hybridization (FISH) in clinical decision making for patients in Acute 
      Promyelocytic Leukemia (APL).
PG  - 515-20
LID - 10.1111/ijlh.12326 [doi]
AB  - INTRODUCTION: Acute Promyelocytic Leukemia (APL) is a curable malignancy with 
      studies showing above 90% survival. However, population-based studies looking at 
      survival suggest that approximately 30% of patients with APL die during 
      induction. Early demonstration of t(15;17) will lead to accurate decision making 
      regarding treatment. The aim of this project was to validate earlier time frames 
      for the Abbott Molecular Vysis LSI promyelocytic leukemia (PML)/ retinoic acid 
      receptor alpha (RARA) fluorescence in situ hybridization (FISH) probe (ASR 6-16 
      h). METHODS: Twenty patients (15 APL cases and five non-APL cases) were selected 
      for validating various hybridization times for the FISH probe. Expected normal 
      signal pattern was two red and two green signals (2R2G), and the most common 
      expected abnormal signal pattern was two fusion (yellow) signals, one red and one 
      green (2F1R1G) and/or one fusion, one red and one green (1F1R1G). RESULTS: The 
      specificity of the probe ranged from 84% at 2 h, 86% at 4 h, 84% at 6 h, and 87% 
      for overnight hybridization. The sensitivity increased from 79% at 2 h, 80% at 4 
      h, 81% at 6 h to 87% for overnight hybridization. CONCLUSION: Based on the 
      validation studies, we recommend reading of FISH results at the 4-h incubation 
      mark for a preliminary diagnosis and confirmation with overnight hybridization.
CI  - (c) 2015 John Wiley & Sons Ltd.
FAU - Kolhe, R
AU  - Kolhe R
AUID- ORCID: 0000-0002-8283-2403
AD  - Department of Pathology, Georgia Regents University, Augusta, GA, USA.
FAU - Mangaonkar, A
AU  - Mangaonkar A
AD  - Department of Internal Medicine, Georgia Regents University Cancer Center, 
      Augusta, GA, USA.
FAU - Mansour, J
AU  - Mansour J
AD  - Department of Internal Medicine, Georgia Regents University Cancer Center, 
      Augusta, GA, USA.
FAU - Clemmons, A
AU  - Clemmons A
AD  - University of Georgia College of Pharmacy, Augusta, GA, USA.
FAU - Shaw, J
AU  - Shaw J
AD  - Department of Pathology, Georgia Regents University, Augusta, GA, USA.
FAU - Dupont, B
AU  - Dupont B
AD  - Department of Pathology, Georgia Regents University, Augusta, GA, USA.
FAU - Walczak, L
AU  - Walczak L
AD  - Department of Pathology, Georgia Regents University, Augusta, GA, USA.
FAU - Mondal, A
AU  - Mondal A
AD  - Department of Pathology, Georgia Regents University, Augusta, GA, USA.
FAU - Rojiani, A
AU  - Rojiani A
AD  - Department of Pathology, Georgia Regents University, Augusta, GA, USA.
FAU - Jillella, A
AU  - Jillella A
AD  - Winship Cancer Institute of Emory University, Atlanta, GA, USA.
FAU - Kota, V
AU  - Kota V
AD  - Winship Cancer Institute of Emory University, Atlanta, GA, USA.
LA  - eng
PT  - Journal Article
DEP - 20150114
PL  - England
TA  - Int J Lab Hematol
JT  - International journal of laboratory hematology
JID - 101300213
RN  - 0 (RARA protein, human)
RN  - 0 (Receptors, Retinoic Acid)
RN  - 0 (Retinoic Acid Receptor alpha)
SB  - IM
MH  - Bone Marrow/metabolism/pathology
MH  - Case-Control Studies
MH  - Chromosomes, Human, Pair 15
MH  - Chromosomes, Human, Pair 17
MH  - Clinical Decision-Making
MH  - Early Diagnosis
MH  - Gene Expression
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Promyelocytic, Acute/*diagnosis/*genetics/pathology
MH  - Leukocytes, Mononuclear/metabolism/pathology
MH  - Lymph Nodes/metabolism/pathology
MH  - Receptors, Retinoic Acid/*genetics
MH  - Retinoic Acid Receptor alpha
MH  - Sensitivity and Specificity
MH  - Time Factors
MH  - *Translocation, Genetic
OTO - NOTNLM
OT  - Acute promyelocytic leukemia
OT  - Fluorescence In Situ Hybridization
OT  - early diagnosis
EDAT- 2015/02/03 06:00
MHDA- 2016/04/14 06:00
CRDT- 2015/02/03 06:00
PHST- 2014/05/14 00:00 [received]
PHST- 2014/11/05 00:00 [accepted]
PHST- 2015/02/03 06:00 [entrez]
PHST- 2015/02/03 06:00 [pubmed]
PHST- 2016/04/14 06:00 [medline]
AID - 10.1111/ijlh.12326 [doi]
PST - ppublish
SO  - Int J Lab Hematol. 2015 Aug;37(4):515-20. doi: 10.1111/ijlh.12326. Epub 2015 Jan 
      14.