PMID- 2564035
OWN - NLM
STAT- MEDLINE
DCOM- 19890412
LR  - 20190908
IS  - 0955-3002 (Print)
IS  - 0955-3002 (Linking)
VI  - 55
IP  - 3
DP  - 1989 Mar
TI  - Radiation-induced DNA damage and repair in quiescent and proliferating human 
      tumor cells in vitro.
PG  - 385-95
AB  - The purpose of this study was to examine radiation-induced DNA strand breakage 
      and repair in quiescent and proliferating human tumor cells in vitro and 
      determine their relationship to radiation sensitivity and potentially lethal 
      damage repair (PLDR). Using centrifugal elutriation we have isolated from fed 
      plateau-phase cultures of HEp-3 human squamous carcinoma cells, relatively pure 
      populations of quiescent and proliferating cells. This was confirmed by both 
      [3H]-thymidine labelling and acridine orange (AO) staining with flow cytometry. 
      Quiescent cells were more sensitive to ionizing radiation (Do = 0.97 Gy) than 
      were proliferating cells (Do = 1.28 Gy). However, quiescent cells showed higher 
      repair of potentially lethal damage (PLDR) than did proliferating cells. Repair 
      of single-strand breaks (ssb) and double-strand breaks (dsb) as measured by 
      filter elution did not differ significantly between quiescent and proliferating 
      cells. For both populations, ssb and dsb repair kinetics and final damage 
      remaining were the same, suggesting that repair of DNA strand breaks is not 
      entirely responsible for the difference in radiation sensitivity between 
      quiescent and proliferating cells.
FAU - Wilson, K M
AU  - Wilson KM
AD  - University of Rochester Cancer Center, NY 14642.
FAU - Keng, P C
AU  - Keng PC
LA  - eng
GR  - CA11051/CA/NCI NIH HHS/United States
GR  - CA11198/CA/NCI NIH HHS/United States
GR  - CA44723/CA/NCI NIH HHS/United States
GR  - etc.
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Int J Radiat Biol
JT  - International journal of radiation biology
JID - 8809243
RN  - 0 (DNA, Neoplasm)
RN  - 0 (DNA, Single-Stranded)
SB  - IM
MH  - Carcinoma, Squamous Cell/pathology
MH  - Cell Division
MH  - Cell Line
MH  - *DNA Damage
MH  - *DNA Repair
MH  - DNA, Neoplasm/*radiation effects
MH  - DNA, Single-Stranded/radiation effects
MH  - Humans
MH  - In Vitro Techniques
MH  - Radiation Tolerance
EDAT- 1989/03/01 00:00
MHDA- 1989/03/01 00:01
CRDT- 1989/03/01 00:00
PHST- 1989/03/01 00:00 [pubmed]
PHST- 1989/03/01 00:01 [medline]
PHST- 1989/03/01 00:00 [entrez]
AID - D9M09KVF6CDCFU1H [pii]
AID - 10.1080/09553008914550431 [doi]
PST - ppublish
SO  - Int J Radiat Biol. 1989 Mar;55(3):385-95. doi: 10.1080/09553008914550431.