PMID- 25640723
OWN - NLM
STAT- MEDLINE
DCOM- 20151026
LR  - 20150202
IS  - 1879-0909 (Electronic)
IS  - 0141-0229 (Linking)
VI  - 69
DP  - 2015 Feb
TI  - Enhancing GDP-fucose production in recombinant Escherichia coli by metabolic 
      pathway engineering.
PG  - 38-45
LID - S0141-0229(14)00211-7 [pii]
LID - 10.1016/j.enzmictec.2014.12.001 [doi]
AB  - Guanosine 5'-diphosphate (GDP)-fucose is the indispensible donor substrate for 
      fucosyltransferase-catalyzed synthesis of fucose-containing biomolecules, which 
      have been found involving in various biological functions. In this work, the 
      salvage pathway for GDP-fucose biosynthesis from Bacterioides fragilis was 
      introduced into Escherichia coli. Besides, the biosynthesis of guanosine 
      5'-triphosphate (GTP), an essential substrate for GDP-fucose biosynthesis, was 
      enhanced via overexpression of enzymes involved in the salvage pathway of GTP 
      biosynthesis. The production capacities of metabolically engineered strains 
      bearing different combinations of recombinant enzymes were compared. The shake 
      flask fermentation of the strain expressing Fkp, Gpt, Gmk and Ndk obtained the 
      maximum GDP-fucose content of 4.6 +/- 0.22 mumol/g (dry cell mass), which is 4.2 
      fold that of the strain only expressing Fkp. Through fed-batch fermentation, the 
      GDP-fucose content further rose to 6.6 +/- 0.14 mumol/g (dry cell mass). In addition 
      to a better productivity than previous fermentation processes based on the de 
      novo pathway for GDP-fucose biosynthesis, the established schemes in this work 
      also have the advantage to be a potential avenue to GDP-fucose analogs 
      encompassing chemical modification on the fucose residue.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Zhai, Yafei
AU  - Zhai Y
AD  - National Glycoengineering Research Center, Shandong University, Jinan, Shandong 
      250100, People's Republic of China; The State Key Laboratory of Microbial 
      Technology and School of Life Science, Shandong University, Jinan, Shandong 
      250100, People's Republic of China.
FAU - Han, Donglei
AU  - Han D
AD  - National Glycoengineering Research Center, Shandong University, Jinan, Shandong 
      250100, People's Republic of China; The State Key Laboratory of Microbial 
      Technology and School of Life Science, Shandong University, Jinan, Shandong 
      250100, People's Republic of China.
FAU - Pan, Ying
AU  - Pan Y
AD  - The State Key Laboratory of Microbial Technology and School of Life Science, 
      Shandong University, Jinan, Shandong 250100, People's Republic of China.
FAU - Wang, Shuaishuai
AU  - Wang S
AD  - National Glycoengineering Research Center, Shandong University, Jinan, Shandong 
      250100, People's Republic of China; The State Key Laboratory of Microbial 
      Technology and School of Life Science, Shandong University, Jinan, Shandong 
      250100, People's Republic of China.
FAU - Fang, Junqiang
AU  - Fang J
AD  - National Glycoengineering Research Center, Shandong University, Jinan, Shandong 
      250100, People's Republic of China.
FAU - Wang, Peng
AU  - Wang P
AD  - National Glycoengineering Research Center, Shandong University, Jinan, Shandong 
      250100, People's Republic of China.
FAU - Liu, Xian-wei
AU  - Liu XW
AD  - National Glycoengineering Research Center, Shandong University, Jinan, Shandong 
      250100, People's Republic of China. Electronic address: xianweiliu@sdu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141209
PL  - United States
TA  - Enzyme Microb Technol
JT  - Enzyme and microbial technology
JID - 8003761
RN  - 0 (Bacterial Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 15839-70-0 (Guanosine Diphosphate Fucose)
RN  - EC 2.7.1.- (Phosphotransferases (Alcohol Group Acceptor))
RN  - EC 2.7.1.52 (fucokinase)
RN  - EC 2.7.7.- (Nucleotidyltransferases)
RN  - EC 2.7.7.30 (fucose-1-phosphate guanylyltransferase)
SB  - IM
MH  - Bacterial Proteins/genetics/metabolism
MH  - Bacteroides fragilis/enzymology/genetics
MH  - Escherichia coli/genetics/growth & development/metabolism
MH  - Fermentation
MH  - Genetic Engineering
MH  - Guanosine Diphosphate Fucose/*biosynthesis
MH  - Metabolic Engineering
MH  - Metabolic Networks and Pathways
MH  - Nucleotidyltransferases/genetics/metabolism
MH  - Phosphotransferases (Alcohol Group Acceptor)/genetics/metabolism
MH  - Recombinant Proteins/genetics/metabolism
OTO - NOTNLM
OT  - Escherichia coli
OT  - GDP-fucose
OT  - Guanosine nucleotides
OT  - Metabolic engineering
OT  - Salvage pathway
EDAT- 2015/02/03 06:00
MHDA- 2015/10/27 06:00
CRDT- 2015/02/03 06:00
PHST- 2014/08/06 00:00 [received]
PHST- 2014/10/10 00:00 [revised]
PHST- 2014/12/01 00:00 [accepted]
PHST- 2015/02/03 06:00 [entrez]
PHST- 2015/02/03 06:00 [pubmed]
PHST- 2015/10/27 06:00 [medline]
AID - S0141-0229(14)00211-7 [pii]
AID - 10.1016/j.enzmictec.2014.12.001 [doi]
PST - ppublish
SO  - Enzyme Microb Technol. 2015 Feb;69:38-45. doi: 10.1016/j.enzmictec.2014.12.001. 
      Epub 2014 Dec 9.