PMID- 25640983
OWN - NLM
STAT- MEDLINE
DCOM- 20160321
LR  - 20181202
IS  - 1365-2672 (Electronic)
IS  - 1364-5072 (Linking)
VI  - 118
IP  - 4
DP  - 2015 Apr
TI  - Ellagic acid derivatives from Terminalia chebula Retz. increase the 
      susceptibility of Pseudomonas aeruginosa to stress by inhibiting polyphosphate 
      kinase.
PG  - 817-25
LID - 10.1111/jam.12733 [doi]
AB  - AIM: Polyphosphate kinase 1 (PPK1) plays an important role in virulence, 
      antibiotic resistance and survival under stress conditions and, therefore, is an 
      attractive therapeutic target to control infections caused by multiple drug 
      resistant Pseudomonas aeruginosa. This study explores the PPK1 inhibiting 
      activity of ellagic acid derivatives (EADs) from Terminalia chebula Retz. that 
      could increase the susceptibility of Ps. aeruginosa to in vitro stress 
      conditions. METHODS AND RESULTS: EADs reduced ppk1 gene expression by 93% (P < 
      0.05) and completely inhibited its activity (P < 0.01) at 0.5 mg ml(-1) . 
      EADs-treated Ps. aeruginosa showed marked reduction in polyphosphate granules in 
      cytosol. Expression of rpoS, the downstream master stress response regulator, was 
      reduced by 94% (P < 0.05) and the sensitivity of Ps. aeruginosa increased many 
      fold to desiccation, oxidative (H2 O2 ) and antibiotic (piperacillin) stresses. 
      PPK-regulated swimming, swarming and twitching motilities and biofilm formation 
      were also reduced significantly (P </= 0.05) in MPAO1 and the clinical strains of 
      Ps. aeruginosa. CONCLUSION: EADs from T. chebula inhibited PPK1 expression and 
      its activity and increased the sensitivity of Ps. aeruginosa to desiccation and 
      oxidative stress while reducing tolerance to piperacillin. SIGNIFICANCE AND 
      IMPACT OF THE STUDY: The study underlines the potential of EADs as therapeutic 
      agent against Ps. aeruginosa.
CI  - (c) 2014 The Society for Applied Microbiology.
FAU - Sarabhai, S
AU  - Sarabhai S
AD  - Department of Microbiology, Panjab University, Chandigarh, India.
FAU - Harjai, K
AU  - Harjai K
FAU - Sharma, P
AU  - Sharma P
FAU - Capalash, N
AU  - Capalash N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150203
PL  - England
TA  - J Appl Microbiol
JT  - Journal of applied microbiology
JID - 9706280
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Plant Extracts)
RN  - 0 (Polyphosphates)
RN  - 19YRN3ZS9P (Ellagic Acid)
RN  - EC 2.7.4.- (Phosphotransferases (Phosphate Group Acceptor))
RN  - EC 2.7.4.1 (polyphosphate kinase)
SB  - IM
MH  - Anti-Bacterial Agents/*pharmacology
MH  - Bacterial Proteins/antagonists & inhibitors/genetics/*metabolism
MH  - Ellagic Acid/*pharmacology
MH  - Humans
MH  - Phosphotransferases (Phosphate Group Acceptor)/antagonists & 
      inhibitors/genetics/*metabolism
MH  - Plant Extracts/*pharmacology
MH  - Polyphosphates/metabolism
MH  - Pseudomonas aeruginosa/*drug effects/*enzymology/genetics/physiology
MH  - Terminalia/*chemistry
OTO - NOTNLM
OT  - Ps. aeruginosa
OT  - Terminalia chebula
OT  - ellagic acid derivatives
OT  - motilities
OT  - persister cells
OT  - ppk
OT  - rpoS
OT  - stress
EDAT- 2015/02/03 06:00
MHDA- 2016/03/22 06:00
CRDT- 2015/02/03 06:00
PHST- 2014/06/29 00:00 [received]
PHST- 2014/12/16 00:00 [revised]
PHST- 2014/12/16 00:00 [accepted]
PHST- 2015/02/03 06:00 [entrez]
PHST- 2015/02/03 06:00 [pubmed]
PHST- 2016/03/22 06:00 [medline]
AID - 10.1111/jam.12733 [doi]
PST - ppublish
SO  - J Appl Microbiol. 2015 Apr;118(4):817-25. doi: 10.1111/jam.12733. Epub 2015 Feb 
      3.