PMID- 25641597
OWN - NLM
STAT- MEDLINE
DCOM- 20180226
LR  - 20181202
IS  - 1097-0347 (Electronic)
IS  - 1043-3074 (Linking)
VI  - 38 Suppl 1
DP  - 2016 Apr
TI  - Biofilm formation on the Provox ActiValve: Composition and ingrowth analyzed by 
      Illumina paired-end RNA sequencing, fluorescence in situ hybridization, and 
      confocal laser scanning microscopy.
PG  - E432-40
LID - 10.1002/hed.24014 [doi]
AB  - BACKGROUND: The most frequent cause of voice prosthesis failure is microbial 
      biofilm formation on the silicone valve, leading to destruction of the material 
      and transprosthetic leakage. The Provox ActiValve valve is made of fluoroplastic, 
      which should be insusceptible to destruction. The purpose of this study was to 
      determine if fluoroplastic is insusceptible to destruction by Candida species. 
      METHODS: Thirty-three dysfunctional Provox ActiValves (collected 2011-2013). 
      Biofilm analysis was performed with Illumina paired-end sequencing (IPES), 
      assessment of biofilm-material interaction with fluorescence in situ 
      hybridization (FISH), and confocal laser scanning microscopy (CLSM). RESULTS: 
      IPES (n = 10) showed that Candida albicans and Candida tropicalis are dominant 
      populations on fluoroplastic and silicone. Microbial diversity is significantly 
      lower on fluoroplastic. Lactobacillus gasseri is the prevalent bacterial strain 
      on most voice prostheses. FISH and CLSM (n = 23): in none of the cases was 
      ingrowth of Candida species present in the fluoroplastic. CONCLUSION: 
      Fluoroplastic material of Provox ActiValve seems insusceptible to destruction by 
      Candida species, which could help improve durability of voice prostheses. (c) 2015 
      Wiley Periodicals, Inc. Head Neck 38: E432-E440, 2016.
CI  - (c) 2015 Wiley Periodicals, Inc.
FAU - Timmermans, Adriana J
AU  - Timmermans AJ
AD  - Department of Head and Neck Oncology and Surgery, The Netherlands Cancer 
      Institute, Amsterdam, The Netherlands.
FAU - Harmsen, Hermie J M
AU  - Harmsen HJ
AD  - Department of Medical Microbiology, University of Groningen, University Medical 
      Center, Groningen, Groningen, The Netherlands.
FAU - Bus-Spoor, Carien
AU  - Bus-Spoor C
AD  - Department of Medical Microbiology, University of Groningen, University Medical 
      Center, Groningen, Groningen, The Netherlands.
FAU - Buijssen, Kevin J D A
AU  - Buijssen KJ
AD  - Department of Otorhinolaryngology, Albert Schweitzer Hospital, Dordrecht, The 
      Netherlands.
FAU - van As-Brooks, Corina
AU  - van As-Brooks C
AD  - Department of Head and Neck Oncology and Surgery, The Netherlands Cancer 
      Institute, Amsterdam, The Netherlands.
AD  - Department of Clinical Affairs, Atos Medical AB, Horby, Sweden.
FAU - de Goffau, Marcus C
AU  - de Goffau MC
AD  - Department of Medical Microbiology, University of Groningen, University Medical 
      Center, Groningen, Groningen, The Netherlands.
FAU - Tonk, Rudi H
AU  - Tonk RH
AD  - Department of Medical Microbiology, University of Groningen, University Medical 
      Center, Groningen, Groningen, The Netherlands.
FAU - van den Brekel, Michiel W M
AU  - van den Brekel MW
AD  - Department of Head and Neck Oncology and Surgery, The Netherlands Cancer 
      Institute, Amsterdam, The Netherlands.
AD  - Institute of Phonetic Sciences, University of Amsterdam, Amsterdam, The 
      Netherlands.
AD  - Department of Oral and Maxillofacial Surgery, Academic Medical Center, Amsterdam, 
      The Netherlands.
FAU - Hilgers, Frans J M
AU  - Hilgers FJ
AD  - Department of Head and Neck Oncology and Surgery, The Netherlands Cancer 
      Institute, Amsterdam, The Netherlands.
AD  - Institute of Phonetic Sciences, University of Amsterdam, Amsterdam, The 
      Netherlands.
AD  - Department of Oral and Maxillofacial Surgery, Academic Medical Center, Amsterdam, 
      The Netherlands.
FAU - van der Laan, Bernard F A M
AU  - van der Laan BF
AD  - Department of Otorhinolaryngology and Head and Neck Oncology and Surgery, 
      University of Groningen, University Medical Center, Groningen, Groningen, The 
      Netherlands.
LA  - eng
PT  - Journal Article
DEP - 20150714
PL  - United States
TA  - Head Neck
JT  - Head & neck
JID - 8902541
RN  - 0 (Plastics)
SB  - IM
MH  - Adult
MH  - Aged
MH  - *Biofilms
MH  - Candida/isolation & purification
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lactobacillus/isolation & purification
MH  - Laryngectomy
MH  - Larynx, Artificial/*microbiology
MH  - Male
MH  - Microscopy, Confocal
MH  - Middle Aged
MH  - Plastics
MH  - Prosthesis Design
MH  - Sequence Analysis, RNA
OTO - NOTNLM
OT  - Candida
OT  - biofilm
OT  - total laryngectomy
OT  - voice prosthesis
OT  - voice rehabilitation
EDAT- 2015/02/03 06:00
MHDA- 2018/02/27 06:00
CRDT- 2015/02/03 06:00
PHST- 2014/05/30 00:00 [received]
PHST- 2014/12/18 00:00 [revised]
PHST- 2015/01/06 00:00 [accepted]
PHST- 2015/02/03 06:00 [entrez]
PHST- 2015/02/03 06:00 [pubmed]
PHST- 2018/02/27 06:00 [medline]
AID - 10.1002/hed.24014 [doi]
PST - ppublish
SO  - Head Neck. 2016 Apr;38 Suppl 1:E432-40. doi: 10.1002/hed.24014. Epub 2015 Jul 14.