PMID- 25642221
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150202
LR  - 20200930
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 5
DP  - 2014
TI  - Chemoautotrophic growth of ammonia-oxidizing Thaumarchaeota enriched from a 
      pelagic redox gradient in the Baltic Sea.
PG  - 786
LID - 10.3389/fmicb.2014.00786 [doi]
LID - 786
AB  - Ammonia-oxidizing archaea (AOA) are an important component of the planktonic 
      community in aquatic habitats, linking nitrogen and carbon cycles through 
      nitrification and carbon fixation. Therefore, measurements of these processes in 
      culture-based experiments can provide insights into their contributions to energy 
      conservation and biomass production by specific AOA. In this study, by enriching 
      AOA from a brackish, oxygen-depleted water-column in the Landsort Deep, central 
      Baltic Sea, we were able to investigate ammonium oxidation, chemoautotrophy, and 
      growth in seawater batch experiments. The highly enriched culture consisted of up 
      to 97% archaea, with maximal archaeal numbers of 2.9 x 10(7) cells mL(-1). 
      Phylogenetic analysis of the 16S rRNA and ammonia monooxygenase subunit A (amoA) 
      gene sequences revealed an affiliation with assemblages from low-salinity and 
      freshwater habitats, with Candidatus Nitrosoarchaeum limnia as the closest 
      relative. Growth correlated significantly with nitrite production, ammonium 
      consumption, and CO2 fixation, which occurred at a ratio of 10 atoms N oxidized 
      per 1 atom C fixed. According to the carbon balance, AOA biomass production can 
      be entirely explained by chemoautotrophy. The cellular carbon content was 
      estimated to be 9 fg C per cell. Single-cell-based (13)C and (15)N labeling 
      experiments and analysis by nano-scale secondary ion mass spectrometry provided 
      further evidence that cellular carbon was derived from bicarbonate and that 
      ammonium was taken up by the cells. Our study therefore revealed that growth by 
      an AOA belonging to the genus Nitrosoarchaeum can be sustained largely by 
      chemoautotrophy.
FAU - Berg, Carlo
AU  - Berg C
AD  - Biological Oceanography, Leibniz Institute for Baltic Sea Research Warnemunde 
      (IOW) Rostock, Germany.
FAU - Listmann, Luisa
AU  - Listmann L
AD  - Biological Oceanography, Leibniz Institute for Baltic Sea Research Warnemunde 
      (IOW) Rostock, Germany.
FAU - Vandieken, Verona
AU  - Vandieken V
AD  - Biological Oceanography, Leibniz Institute for Baltic Sea Research Warnemunde 
      (IOW) Rostock, Germany ; Paleomicrobiology Group, Institute for Chemistry and 
      Biology of the Marine Environment, Carl von Ossietzky University of Oldenburg 
      Oldenburg, Germany.
FAU - Vogts, Angela
AU  - Vogts A
AD  - Biological Oceanography, Leibniz Institute for Baltic Sea Research Warnemunde 
      (IOW) Rostock, Germany.
FAU - Jurgens, Klaus
AU  - Jurgens K
AD  - Biological Oceanography, Leibniz Institute for Baltic Sea Research Warnemunde 
      (IOW) Rostock, Germany.
LA  - eng
PT  - Journal Article
DEP - 20150115
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC4295551
OTO - NOTNLM
OT  - Baltic Sea
OT  - CO2-fixation
OT  - Thaumarchaeota
OT  - ammonia-oxidizing archaea
OT  - chemoautotrophy
OT  - enrichment
EDAT- 2015/02/03 06:00
MHDA- 2015/02/03 06:01
PMCR- 2015/01/15
CRDT- 2015/02/03 06:00
PHST- 2014/10/01 00:00 [received]
PHST- 2014/12/21 00:00 [accepted]
PHST- 2015/02/03 06:00 [entrez]
PHST- 2015/02/03 06:00 [pubmed]
PHST- 2015/02/03 06:01 [medline]
PHST- 2015/01/15 00:00 [pmc-release]
AID - 10.3389/fmicb.2014.00786 [doi]
PST - epublish
SO  - Front Microbiol. 2015 Jan 15;5:786. doi: 10.3389/fmicb.2014.00786. eCollection 
      2014.