PMID- 25642885
OWN - NLM
STAT- MEDLINE
DCOM- 20150326
LR  - 20150203
IS  - 1555-9823 (Electronic)
IS  - 0003-1348 (Linking)
VI  - 81
IP  - 2
DP  - 2015 Feb
TI  - Changes in fecal and colonic mucosal microbiota of patients with refractory 
      constipation after a subtotal colectomy.
PG  - 198-206
AB  - The purpose of this study was to investigate the changes in gut microbiota of 
      patients with refractory constipation 6 months after a subtotal colectomy. Feces 
      and mucosal samples of five healthy volunteers and 17 patients with refractory 
      constipation before and six months after subtotal colectomy were collected. 
      Denaturing gradient gel electrophoresis (DGGE) and polymerase chain reaction 
      techniques were used for quantitative analysis of main bacterial groups and 
      archeal methanogens. No significant differences were found in the DGGE profiles 
      among the three groups. After subtotal colectomy, a significantly decreased 
      similarity coefficient was observed in the fecal. The Shannon diversity indices 
      had no significant differences among the three groups. The numbers of predominant 
      bacteria (Bacteriodetes, Clostridium coccoides group, and Clostridium leptum 
      group) did not significantly change in patients before and after surgery compared 
      with healthy control subjects, and the number of total bacteria, Firmicutes, 
      Bacteriodetes, and Clostridium leptum group bacteria in the feces decreased after 
      surgery. However, the numbers of these bacteria remained the same in mucosa from 
      postoperative patients. The numbers of Bifidobacteria and Lactobacilli in feces 
      and the number of Bifidobacteria in mucosa were significantly lower in 
      preoperative compared with healthy control subjects and increased after the 
      surgery. The number of methanogens in the mucosa was decreased in preoperative 
      patients but returned to normal levels postoperatively. In conclusion, although 
      there was no difference in the structure of the predominant bacteria between 
      refractory constipated patients and healthy control subjects, the number of 
      probiotics (Bifidobacteria and Lactobacilli) was significantly lower in 
      refractory constipated patients. However, subtotal colectomy can significantly 
      normalize the number of intestinal flora.
FAU - Feng, Xiaobo
AU  - Feng X
AD  - Department of General Surgery, JinLing Hospital, Medical School of Nanjing 
      University, Nanjing, China.
FAU - Su, Yong
AU  - Su Y
FAU - Jiang, Jun
AU  - Jiang J
FAU - Li, Ning
AU  - Li N
FAU - Ding, Weiwei
AU  - Ding W
FAU - Wang, Zhiming
AU  - Wang Z
FAU - Hu, Xionghui
AU  - Hu X
FAU - Zhu, Weiyun
AU  - Zhu W
FAU - Li, Jieshou
AU  - Li J
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am Surg
JT  - The American surgeon
JID - 0370522
SB  - IM
MH  - Adult
MH  - Colectomy/*methods
MH  - Colon/*microbiology
MH  - Constipation/*etiology/*microbiology
MH  - Denaturing Gradient Gel Electrophoresis
MH  - Feces/*microbiology
MH  - Female
MH  - Humans
MH  - Intestinal Mucosa/*microbiology
MH  - Male
MH  - *Microbiota
MH  - Middle Aged
MH  - Polymerase Chain Reaction
EDAT- 2015/02/03 06:00
MHDA- 2015/03/27 06:00
CRDT- 2015/02/03 06:00
PHST- 2015/02/03 06:00 [entrez]
PHST- 2015/02/03 06:00 [pubmed]
PHST- 2015/03/27 06:00 [medline]
PST - ppublish
SO  - Am Surg. 2015 Feb;81(2):198-206.