PMID- 25643830
OWN - NLM
STAT- MEDLINE
DCOM- 20160121
LR  - 20160511
IS  - 2042-6984 (Electronic)
IS  - 2042-6976 (Linking)
VI  - 5
IP  - 4
DP  - 2015 Apr
TI  - Topical colloidal silver as an anti-biofilm agent in a Staphylococcus aureus 
      chronic rhinosinusitis sheep model.
PG  - 283-8
LID - 10.1002/alr.21459 [doi]
AB  - BACKGROUND: Treatment of recalcitrant chronic rhinosinusitis (CRS) is a challenge 
      with increasing antibiotic resistance, leading to re-emergence of topical 
      therapies. The aim of this study was to assess safety and efficacy of topical 
      colloidal silver solution for the treatment of Staphylococcus aureus biofilms in 
      a sheep model. METHODS: In the safety study, normal saline (control) and 30-ppm 
      colloidal silver solution (test) was used to flush the frontal sinuses for 14 
      days in 8 sheep (4 sheep each). In the efficacy study, following frontal sinus 
      infection with Staphylococcus aureus, sheep were treated with either control 
      saline or topical silver solution of varying concentrations (30 ppm/20 ppm/10 
      ppm/5 ppm) for 5 days, with 4 sheep in each group. Blood silver level, full blood 
      counts, and biochemical parameters were analyzed in both safety and efficacy 
      studies. Sinus tissue was harvested for histological examination and ciliary 
      structure analysis in safety and for biofilm biomass quantification by 
      fluorescence in situ hybridization (FISH) technique and COMSTAT 2 software in the 
      efficacy study. Results were analyzed using appropriate statistical tests. 
      RESULTS: Sheep treated with silver showed a significant decrease in biofilm 
      biomass (0.004, 0.004, 0.004, and 0.007, in the 4 silver-treated groups, 
      respectively) compared to saline control (0.175), p < 0.001. Although average 
      blood silver levels were higher in the treated groups compared to controls (p < 
      0.05), blood counts and biochemical parameters were normal. Histology and ciliary 
      structure analysis did not show any difference between control and treatment 
      groups. CONCLUSION: Topical colloidal silver solution has effective antibiofilm 
      activity in Staphylococcus aureus CRS in a sheep model and appears safe.
CI  - (c) 2015 ARS-AAOA, LLC.
FAU - Rajiv, Sukanya
AU  - Rajiv S
AD  - Department of Surgery-Otorhinolaryngology, Head and Neck Surgery, University of 
      Adelaide, Adelaide, Australia.
FAU - Drilling, Amanda
AU  - Drilling A
FAU - Bassiouni, Ahmed
AU  - Bassiouni A
FAU - James, Craig
AU  - James C
FAU - Vreugde, Sarah
AU  - Vreugde S
FAU - Wormald, Peter-John
AU  - Wormald PJ
LA  - eng
PT  - Journal Article
DEP - 20150130
PL  - United States
TA  - Int Forum Allergy Rhinol
JT  - International forum of allergy & rhinology
JID - 101550261
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (colloidal silver)
RN  - 3M4G523W1G (Silver)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/therapeutic use
MH  - Biofilms/*drug effects
MH  - *Disease Models, Animal
MH  - Rhinitis/*drug therapy/microbiology
MH  - Sheep
MH  - Silver/*therapeutic use
MH  - Sinusitis/*drug therapy/microbiology
MH  - Staphylococcal Infections/*drug therapy/microbiology
MH  - Staphylococcus aureus/*physiology
MH  - Treatment Outcome
OTO - NOTNLM
OT  - Staphylococcus aureus
OT  - biofilm
OT  - chronic rhinosinusitis
OT  - colloidal silver
OT  - sheep
EDAT- 2015/02/04 06:00
MHDA- 2016/01/23 06:00
CRDT- 2015/02/04 06:00
PHST- 2014/08/20 00:00 [received]
PHST- 2014/10/16 00:00 [revised]
PHST- 2014/10/21 00:00 [accepted]
PHST- 2015/02/04 06:00 [entrez]
PHST- 2015/02/04 06:00 [pubmed]
PHST- 2016/01/23 06:00 [medline]
AID - 10.1002/alr.21459 [doi]
PST - ppublish
SO  - Int Forum Allergy Rhinol. 2015 Apr;5(4):283-8. doi: 10.1002/alr.21459. Epub 2015 
      Jan 30.