PMID- 25644064
OWN - NLM
STAT- MEDLINE
DCOM- 20160113
LR  - 20181113
IS  - 1097-4547 (Electronic)
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 93
IP  - 6
DP  - 2015 Jun
TI  - Temporal changes in neurotrophic factors and neurite outgrowth in the major 
      pelvic ganglion following cavernous nerve injury.
PG  - 954-63
LID - 10.1002/jnr.23553 [doi]
AB  - Despite nerve-sparing radical prostatectomy, nerve damage and erectile 
      dysfunction (ED) prevail, and preventing neurodegeneration is of great 
      importance. Neurotrophic factors and neurite outgrowth were characterized in 
      major pelvic ganglia (MPG) following bilateral cavernous nerve injury (BCNI). 
      Young male Sprague-Dawley rats underwent sham or BCNI surgery, and the 
      intracavernosal pressure to mean arterial pressure ratio was measured 2, 7, 14, 
      21, 30, and 60 days following injury (n = 8/group). MPG gene expression (qPCR) 
      and Western blot were performed for glial cell line-derived neurotrophic factor 
      (GDNF), nerve growth factor (NGF), neurturin, neurotrophin (NT)-3, NT4, 
      brain-derived neurotrophic factor (BDNF), vascular endothelial growth factor, and 
      activating transcription factor 3 (ATF3). Additional rats were injured, and MPGs 
      were removed 24 hr, 48 hr, 3 days, and 7 days following BCNI (n = 3/group). MPGs 
      were cultured in Matrigel, and neurite outgrowth was measured. Erections were 
      impaired early and improved by 60 days in BCNI rats. GDNF, NGF, BDNF, and ATF3 
      gene expression was significantly increased and NT3 was decreased in MPGs 
      following BCNI (48 hr to 21 days, P < 0.05). GDNF and NGF protein levels were 
      elevated in 48-hr BCNI rats. MPG neurite outgrowth from 24-hr and 48-hr BCNI was 
      higher than sham (658 +/- 19 mum, 607 +/- 24 mum, 393 +/- 23 mum, respectively, P < 0.05). 
      Further studies examining the roles of neurotrophic factors in modulating 
      signaling pathways may provide therapeutic avenues for neurogenically mediated 
      ED.
CI  - (c) 2015 Wiley Periodicals, Inc.
FAU - Hannan, Johanna L
AU  - Hannan JL
AD  - The James Buchanan Brady Urological Institute and Department of Urology, Johns 
      Hopkins School of Medicine, Baltimore, Maryland.
FAU - Albersen, Maarten
AU  - Albersen M
FAU - Stopak, Bernard L
AU  - Stopak BL
FAU - Liu, Xiaopu
AU  - Liu X
FAU - Burnett, Arthur L
AU  - Burnett AL
FAU - Hoke, Ahmet
AU  - Hoke A
FAU - Bivalacqua, Trinity J
AU  - Bivalacqua TJ
LA  - eng
GR  - K08 DK090370/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20150119
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Nerve Growth Factors)
RN  - 0 (RNA, Messenger)
RN  - EC 2.4.2.8 (Hypoxanthine Phosphoribosyltransferase)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Blood Pressure/physiology
MH  - Cell Culture Techniques
MH  - Disease Models, Animal
MH  - Erectile Dysfunction/etiology
MH  - Ganglia, Sympathetic/*metabolism/*pathology
MH  - Gene Expression Regulation/physiology
MH  - Hypoxanthine Phosphoribosyltransferase/metabolism
MH  - Male
MH  - Nerve Growth Factors/genetics/*metabolism
MH  - Neurites/*physiology
MH  - Peripheral Nerve Injuries/complications/*pathology
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Statistics as Topic
PMC - PMC4397125
MID - NIHMS651257
OTO - NOTNLM
OT  - activating transcription factor 3
OT  - erectile dysfunction
OT  - neurite outgrowth
OT  - peripheral nerve injury
EDAT- 2015/02/04 06:00
MHDA- 2016/01/14 06:00
PMCR- 2016/06/01
CRDT- 2015/02/04 06:00
PHST- 2014/10/15 00:00 [received]
PHST- 2014/11/25 00:00 [revised]
PHST- 2014/12/16 00:00 [accepted]
PHST- 2015/02/04 06:00 [entrez]
PHST- 2015/02/04 06:00 [pubmed]
PHST- 2016/01/14 06:00 [medline]
PHST- 2016/06/01 00:00 [pmc-release]
AID - 10.1002/jnr.23553 [doi]
PST - ppublish
SO  - J Neurosci Res. 2015 Jun;93(6):954-63. doi: 10.1002/jnr.23553. Epub 2015 Jan 19.