PMID- 25644303
OWN - NLM
STAT- MEDLINE
DCOM- 20160531
LR  - 20171116
IS  - 2042-7158 (Electronic)
IS  - 0022-3573 (Linking)
VI  - 67
IP  - 5
DP  - 2015 May
TI  - The modulatory effects of caffeic acid on human monocytes and its involvement in 
      propolis action.
PG  - 740-5
LID - 10.1111/jphp.12364 [doi]
AB  - OBJECTIVES: Researchers have been interested in investigating the mechanisms of 
      action of propolis and the compounds involved in its biological activity; 
      however, the effect of its isolated constituents on human immune cells still 
      deserves investigation. Thus, this study aimed to verify the action of caffeic 
      acid on human monocytes in an attempt to verify its effects on the innate 
      immunity, and to analyse its participation in propolis activity. METHODS: 
      Monocytes viability was assessed by 
      3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method after 
      incubation with caffeic acid. Cell markers expression by monocytes (Toll-like 
      receptors (TLR)-2, TLR-4, human leukocyte antigen (HLA)-DR and CD80) was analysed 
      by flow cytometry. TNF-alpha and IL-10 production was determined by enzyme-linked 
      immunosorbent assay and the activity of monocytes against Candida albicans was 
      investigated after incubation with different concentrations of caffeic acid. KEY 
      FINDINGS: Caffeic acid downregulated TLR-2 and HLA-DR expression and inhibited 
      cytokine production whereas it upregulated the fungicidal activity of monocytes, 
      without affecting cell viability. CONCLUSIONS: Caffeic acid exerted an 
      immunomodulatory action in human monocytes in the evaluated parameters depending 
      on concentration, with no cytotoxic effects. Moreover, it was partially involved 
      in propolis action.
CI  - (c) 2015 Royal Pharmaceutical Society.
FAU - Bufalo, Michelle Cristiane
AU  - Bufalo MC
AD  - Department of Microbiology and Immunology, Biosciences Institute, Universidade 
      Estadual Paulista, Botucatu, SP, Brazil.
FAU - Sforcin, Jose Mauricio
AU  - Sforcin JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150123
PL  - England
TA  - J Pharm Pharmacol
JT  - The Journal of pharmacy and pharmacology
JID - 0376363
RN  - 0 (Antifungal Agents)
RN  - 0 (B7-1 Antigen)
RN  - 0 (Caffeic Acids)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 2)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 130068-27-8 (Interleukin-10)
RN  - 9009-62-5 (Propolis)
RN  - U2S3A33KVM (caffeic acid)
SB  - IM
MH  - Antifungal Agents/pharmacology
MH  - B7-1 Antigen/metabolism
MH  - Caffeic Acids/*pharmacology
MH  - Candida albicans
MH  - Cell Survival/drug effects
MH  - Dose-Response Relationship, Drug
MH  - HLA-DR Antigens/metabolism
MH  - Humans
MH  - Interleukin-10/metabolism
MH  - Microbial Sensitivity Tests
MH  - Monocytes/*drug effects/metabolism
MH  - Propolis/*pharmacology
MH  - Toll-Like Receptor 2/metabolism
MH  - Toll-Like Receptor 4
MH  - Tumor Necrosis Factor-alpha/metabolism
OTO - NOTNLM
OT  - Toll-like receptors
OT  - caffeic acid
OT  - co-stimulatory molecules
OT  - cytokine
OT  - propolis
EDAT- 2015/02/04 06:00
MHDA- 2016/06/01 06:00
CRDT- 2015/02/04 06:00
PHST- 2014/08/08 00:00 [received]
PHST- 2014/11/16 00:00 [accepted]
PHST- 2015/02/04 06:00 [entrez]
PHST- 2015/02/04 06:00 [pubmed]
PHST- 2016/06/01 06:00 [medline]
AID - 10.1111/jphp.12364 [doi]
PST - ppublish
SO  - J Pharm Pharmacol. 2015 May;67(5):740-5. doi: 10.1111/jphp.12364. Epub 2015 Jan 
      23.