PMID- 25644701 OWN - NLM STAT- MEDLINE DCOM- 20151229 LR - 20181113 IS - 1943-2631 (Electronic) IS - 0016-6731 (Print) IS - 0016-6731 (Linking) VI - 199 IP - 4 DP - 2015 Apr TI - Complex formation of RNA silencing proteins in the perinuclear region of Neurospora crassa. PG - 1017-21 LID - 10.1534/genetics.115.174623 [doi] AB - In Neurospora, genes not paired during meiosis are targeted by meiotic silencing by unpaired DNA (MSUD). Here, our bimolecular fluorescence complementation (BiFC) study suggests that RNA-directed RNA polymerase, Dicer, Argonaute, and others form a silencing complex in the perinuclear region, with intimate interactions among the majority of them. We have also shown that SAD-2 is likely the anchor for this assembly. CI - Copyright (c) 2015 by the Genetics Society of America. FAU - Decker, Logan M AU - Decker LM AD - Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211. FAU - Boone, Erin C AU - Boone EC AD - Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211. FAU - Xiao, Hua AU - Xiao H AD - Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211. FAU - Shanker, Benjamin S AU - Shanker BS AD - Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211. FAU - Boone, Shannon F AU - Boone SF AD - Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211. FAU - Kingston, Shanika L AU - Kingston SL AD - Department of Biology, Barry University, Miami Shores, Florida 33161. FAU - Lee, Seung A AU - Lee SA AD - Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211. FAU - Hammond, Thomas M AU - Hammond TM AD - School of Biological Sciences, Illinois State University, Normal, Illinois, 61790. FAU - Shiu, Patrick K T AU - Shiu PK AD - Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211 shiup@missouri.edu. LA - eng GR - P01 GM068087/GM/NIGMS NIH HHS/United States GR - R25 GM059244/GM/NIGMS NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20150202 PL - United States TA - Genetics JT - Genetics JID - 0374636 RN - 0 (Argonaute Proteins) RN - 0 (Fungal Proteins) RN - 0 (Karyopherins) RN - EC 3.1.26.3 (Ribonuclease III) SB - IM MH - Argonaute Proteins/genetics/*metabolism MH - Fungal Proteins/genetics/*metabolism MH - *Gene Silencing MH - Karyopherins/genetics/metabolism MH - Neurospora crassa/*genetics MH - Protein Binding MH - Protein Transport MH - Ribonuclease III/genetics/*metabolism PMC - PMC4391574 OTO - NOTNLM OT - Neurospora crassa OT - RNA interference OT - bimolecular fluorescence complementation (BiFC) OT - fluorescent protein tagging OT - meiotic silencing by unpaired DNA (MSUD) EDAT- 2015/02/04 06:00 MHDA- 2015/12/30 06:00 PMCR- 2016/04/01 CRDT- 2015/02/04 06:00 PHST- 2014/11/17 00:00 [received] PHST- 2015/01/27 00:00 [accepted] PHST- 2015/02/04 06:00 [entrez] PHST- 2015/02/04 06:00 [pubmed] PHST- 2015/12/30 06:00 [medline] PHST- 2016/04/01 00:00 [pmc-release] AID - genetics.115.174623 [pii] AID - 174623 [pii] AID - 10.1534/genetics.115.174623 [doi] PST - ppublish SO - Genetics. 2015 Apr;199(4):1017-21. doi: 10.1534/genetics.115.174623. Epub 2015 Feb 2.