PMID- 25646051
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150203
LR  - 20220331
IS  - 0970-1915 (Print)
IS  - 0974-0422 (Electronic)
IS  - 0970-1915 (Linking)
VI  - 30
IP  - 1
DP  - 2015 Jan
TI  - Curcumin Suppresses the Production of Pro-inflammatory Cytokine Interleukin-18 in 
      Lipopolysaccharide Stimulated Murine Macrophage-Like Cells.
PG  - 109-12
LID - 10.1007/s12291-014-0452-2 [doi]
AB  - Curcumin is a major bioactive compound of turmeric that exerts its 
      anti-inflammatory effects by suppressing the many pro-inflammatory cytokines and 
      chemokines in a number of cell types and pathologic conditions. Interleukin-18 
      (IL-18) is a novel pro-inflammatory cytokine which plays an important role not 
      only in generating Th1 responses but also in inducing severe inflammatory 
      reactions. As curcumin induced inhibition of IL-18 production in keratinocytes 
      and mice is well known, effect of curcumin on IL-18 release in macrophages 
      remains unknown. Hence, this present study has been designed to evaluate the 
      effect of curcumin on IL-18 production and necrotic cell death in murine 
      macrophages-like cells treated with or without lipopolysaccharide (LPS). The 
      IL-18 secretion in cell culture supernatants was assayed by enzyme-linked 
      immunosorbent assay and cytotoxicity was determined by lactate dehydrogenase 
      release assay. Our results demonstrate that curcumin significantly inhibited the 
      production of pro-inflammatory cytokine IL-18 in E.coli LPS stimulated murine 
      macrophage-like cells RAW264.7 in a concentration-dependent manner. 
      Interestingly, curcumin had no cytotoxic effect on murine macrophage-like cells. 
      Our findings suggest that curcumin may be used as a potential therapeutic agent 
      for the treatment of inflammatory diseases.
FAU - Yadav, Renu
AU  - Yadav R
AD  - Institute of Life Sciences, Chhatrapati Shahu Ji Maharaj University, Kanpur, 208 
      024 India.
FAU - Jee, Babban
AU  - Jee B
AD  - Institute of Life Sciences, Chhatrapati Shahu Ji Maharaj University, Kanpur, 208 
      024 India ; Department of Microbiology and Molecular Biology, National JALMA 
      Institute for Leprosy and Other Mycobacterial Diseases (ICMR), Agra, 282 001 
      India.
FAU - Awasthi, Sudhir Kumar
AU  - Awasthi SK
AD  - Institute of Life Sciences, Chhatrapati Shahu Ji Maharaj University, Kanpur, 208 
      024 India.
LA  - eng
PT  - Journal Article
DEP - 20140715
PL  - India
TA  - Indian J Clin Biochem
JT  - Indian journal of clinical biochemistry : IJCB
JID - 8708303
PMC - PMC4310836
OTO - NOTNLM
OT  - Curcumin
OT  - IL-18
OT  - Murine macrophage-like cells
OT  - Necrosis
EDAT- 2015/02/04 06:00
MHDA- 2015/02/04 06:01
PMCR- 2016/01/01
CRDT- 2015/02/04 06:00
PHST- 2013/12/06 00:00 [received]
PHST- 2014/05/23 00:00 [accepted]
PHST- 2015/02/04 06:00 [entrez]
PHST- 2015/02/04 06:00 [pubmed]
PHST- 2015/02/04 06:01 [medline]
PHST- 2016/01/01 00:00 [pmc-release]
AID - 452 [pii]
AID - 10.1007/s12291-014-0452-2 [doi]
PST - ppublish
SO  - Indian J Clin Biochem. 2015 Jan;30(1):109-12. doi: 10.1007/s12291-014-0452-2. 
      Epub 2014 Jul 15.