PMID- 25648830
OWN - NLM
STAT- MEDLINE
DCOM- 20150612
LR  - 20171116
IS  - 1522-1555 (Electronic)
IS  - 0193-1849 (Linking)
VI  - 308
IP  - 7
DP  - 2015 Apr 1
TI  - Deleted in breast cancer 1 plays a functional role in adipocyte differentiation.
PG  - E554-61
LID - 10.1152/ajpendo.00286.2014 [doi]
AB  - Genetic deletion of Dbc1 in mice reduced adipose tissue senescence and 
      inflammation while promoting an expansion of this tissue. Here, we aimed to 
      investigate DBC1 mRNA and protein levels in human adipose tissue from subjects 
      with a wide spectrum of fat mass (cohort 1; n = 105) and insulin resistance 
      (cohort 2; n = 47); we also investigated the effects of DBC1 knockdown on 3T3-L1 
      adipocyte differentiation. DBC1 mRNA was relatively abundant in both visceral 
      (VAT) and subcutaneous adipose tissue (SAT) (mainly in the adipocyte fraction), 
      being decreased in adipose tissue from obese compared with lean subjects. In both 
      VAT and SAT, DBC1 mRNA levels were negatively associated with BMI and positively 
      associated with age and the expression of PPARgamma, GLUT4, IRS1, lipogenic (FASN, 
      ACACA), lipid droplet-associated genes (PLIN1, FSP27, ADRP, and TIP47), and 
      lipolytic (ABDH5, AKAP, and PRKACA) genes but negatively associated with ADIPOQ 
      in VAT. DBC1 mRNA and protein levels were increased in the early stages of 
      adipocyte differentiation of human and 3T3-L1 adipocytes. Dbc1 knockdown (KD) 
      with lentivirus led to enhanced adipocyte differentiation, increasing 
      intracellular lipid accumulation and adipogenic gene expression. In conclusion, 
      although DBC1 gene expression was reduced in adipose tissue from obese subjects, 
      it was negatively associated with ADIPOQ gene expression in VAT, suggesting that 
      DBC1 might promote visceral adipose tissue dysfunction. In vitro data supported 
      the antiadipogenic effects of DBC1.
CI  - Copyright (c) 2015 the American Physiological Society.
FAU - Moreno-Navarrete, Jose Maria
AU  - Moreno-Navarrete JM
AD  - Department of Diabetes, Endocrinology, and Nutrition, Institut d'Investigacio 
      Biomedica de Girona, Centro de Investigacion Biomedica en Red Fisiopatologia de 
      Obesidad y Nutricion (CB06/03/010) and Instituto de Salud Carlos III, Girona, 
      Spain.
FAU - Moreno, Maria
AU  - Moreno M
AD  - Department of Diabetes, Endocrinology, and Nutrition, Institut d'Investigacio 
      Biomedica de Girona, Centro de Investigacion Biomedica en Red Fisiopatologia de 
      Obesidad y Nutricion (CB06/03/010) and Instituto de Salud Carlos III, Girona, 
      Spain.
FAU - Vidal, Marta
AU  - Vidal M
AD  - Department of Diabetes, Endocrinology, and Nutrition, Institut d'Investigacio 
      Biomedica de Girona, Centro de Investigacion Biomedica en Red Fisiopatologia de 
      Obesidad y Nutricion (CB06/03/010) and Instituto de Salud Carlos III, Girona, 
      Spain.
FAU - Ortega, Francisco
AU  - Ortega F
AD  - Department of Diabetes, Endocrinology, and Nutrition, Institut d'Investigacio 
      Biomedica de Girona, Centro de Investigacion Biomedica en Red Fisiopatologia de 
      Obesidad y Nutricion (CB06/03/010) and Instituto de Salud Carlos III, Girona, 
      Spain.
FAU - Serrano, Marta
AU  - Serrano M
AD  - Department of Diabetes, Endocrinology, and Nutrition, Institut d'Investigacio 
      Biomedica de Girona, Centro de Investigacion Biomedica en Red Fisiopatologia de 
      Obesidad y Nutricion (CB06/03/010) and Instituto de Salud Carlos III, Girona, 
      Spain.
FAU - Xifra, Gemma
AU  - Xifra G
AD  - Department of Diabetes, Endocrinology, and Nutrition, Institut d'Investigacio 
      Biomedica de Girona, Centro de Investigacion Biomedica en Red Fisiopatologia de 
      Obesidad y Nutricion (CB06/03/010) and Instituto de Salud Carlos III, Girona, 
      Spain.
FAU - Ricart, Wifredo
AU  - Ricart W
AD  - Department of Diabetes, Endocrinology, and Nutrition, Institut d'Investigacio 
      Biomedica de Girona, Centro de Investigacion Biomedica en Red Fisiopatologia de 
      Obesidad y Nutricion (CB06/03/010) and Instituto de Salud Carlos III, Girona, 
      Spain.
FAU - Fernandez-Real, Jose Manuel
AU  - Fernandez-Real JM
AD  - Department of Diabetes, Endocrinology, and Nutrition, Institut d'Investigacio 
      Biomedica de Girona, Centro de Investigacion Biomedica en Red Fisiopatologia de 
      Obesidad y Nutricion (CB06/03/010) and Instituto de Salud Carlos III, Girona, 
      Spain jmfreal@idibgi.org.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150203
PL  - United States
TA  - Am J Physiol Endocrinol Metab
JT  - American journal of physiology. Endocrinology and metabolism
JID - 100901226
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (CCAR2 protein, human)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adaptor Proteins, Signal Transducing/*physiology
MH  - Adipocytes/*physiology
MH  - Adipogenesis/*genetics
MH  - Adult
MH  - Aged
MH  - Animals
MH  - Cell Differentiation/*genetics
MH  - Cells, Cultured
MH  - Diabetes Mellitus, Type 2/genetics/metabolism
MH  - Gene Expression Profiling
MH  - Humans
MH  - Intra-Abdominal Fat/physiology
MH  - Mice
MH  - Middle Aged
MH  - Obesity/genetics/metabolism
OTO - NOTNLM
OT  - adipogenesis
OT  - adipose tissue
OT  - deleted in breast cancer 1
OT  - insulin sensitivity
OT  - obesity
EDAT- 2015/02/05 06:00
MHDA- 2015/06/13 06:00
CRDT- 2015/02/05 06:00
PHST- 2014/06/20 00:00 [received]
PHST- 2015/01/23 00:00 [accepted]
PHST- 2015/02/05 06:00 [entrez]
PHST- 2015/02/05 06:00 [pubmed]
PHST- 2015/06/13 06:00 [medline]
AID - ajpendo.00286.2014 [pii]
AID - 10.1152/ajpendo.00286.2014 [doi]
PST - ppublish
SO  - Am J Physiol Endocrinol Metab. 2015 Apr 1;308(7):E554-61. doi: 
      10.1152/ajpendo.00286.2014. Epub 2015 Feb 3.