PMID- 25649751
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150204
LR  - 20200930
IS  - 1480-9222 (Print)
IS  - 1480-9222 (Electronic)
IS  - 1480-9222 (Linking)
VI  - 17
IP  - 1
DP  - 2015
TI  - Evaluation of method performance for oxidative stress biomarkers in urine and 
      biological variations in urine of patients with type 2 diabetes mellitus and 
      diabetic nephropathy.
PG  - 3
LID - 10.1186/s12575-015-0015-9 [doi]
LID - 3
AB  - BACKGROUND: Oxidative stress biomarkers such as superoxide dismutase (CuZnSOD), 
      catalase (CAT) and malondialdehyde (MDA) play an important role in the 
      pathogenesis or progression of numerous diseases. Data regarding the biological 
      variation and analytical quality specifications (imprecision, bias and total 
      error) for judging the acceptability of method performance for oxidative stress 
      biomarkers in urine are conspicuously lacking in the literature. Such data are 
      important in setting analytical quality specifications, assessing the utility of 
      population reference intervals (index of individuality) and assessing the 
      significance of changes in serial results from an individual (reference change 
      value; RCV). MATERIALS AND METHODS: 20 patients with type 2 diabetes mellitus 
      (T2DM), 20 patients with diabetic nephropathy (DN) and 14 healthy individuals as 
      control were involved in this study. Timed first morning urine samples were taken 
      from patients and healthy groups on the zero, 1st, 3rd, 5th, 7th, 15th and 30th 
      days. Index of individuality and reference change value were calculated from 
      within-subject and between-subject variations. Methods of oxidative stress 
      biomarkers in human blood were adopted in human urine and markers were measured 
      as spectrophotometrically. Also, analytical quality specifications for evaluation 
      of the method performance were established for oxidative stress biomarkers in 
      urine. RESULTS: Within-subject variations of oxidative stress biomarkers were 
      significantly higher in patients with DN and T2DM compared to healthy subjects. 
      MDA showed low individuality, and within-subject variances of MDA were larger 
      than between-subject variances in all groups. However, CAT and CuZnSOD showed 
      strong individuality, but within-subject variances of them were smaller than 
      between-subject variances in all groups. RCVs of all analytes in diabetic 
      patients were relatively higher, because of high within-subject variation, 
      resulting in a higher RCV. Also, the described methodology achieves these goals, 
      with analytical CVs of < 3.5% for all analytes. Goals for bias and total error 
      were 6.0-7.9% and 12.5-23.3%, respectively. CONCLUSIONS: RCVs concept for 
      predicting the clinical status in diabetic patients represents an optimization of 
      laboratory reporting and could be a valuable tool for clinical decision. 
      Furthermore, for oxidative stress biomarkers' measurements in urine, the 
      desirable imprecision goals based on biological variation are obtainable by 
      current methodologies.
FAU - Kurutas, Ergul Belge
AU  - Kurutas EB
AD  - Department of Biochemistry, Sutcu Imam University Faculty of Medicine, Avsar 
      Campus, 46050 Kahramanmaras, Turkey.
FAU - Gumusalan, Yakup
AU  - Gumusalan Y
AD  - Department of Anatomy, Fatih University Faculty of Medicine, Buyukcekmece Campus, 
      34500 Istanbul, Turkey.
FAU - Cetinkaya, Ali
AU  - Cetinkaya A
AD  - Department of Gastroenterology, Sutcu Imam University Faculty of Medicine, 
      Kahramanmaras, Turkey.
FAU - Dogan, Ekrem
AU  - Dogan E
AD  - Department of Nephrology, Selahaddin Eyyubi University Faculty of Medicine, 
      Baglar Campus, 21090 Diyarbakir, Turkey.
LA  - eng
PT  - Journal Article
DEP - 20150202
PL  - England
TA  - Biol Proced Online
JT  - Biological procedures online
JID - 100963717
PMC - PMC4313470
OTO - NOTNLM
OT  - Biological variation
OT  - DN
OT  - Oxidative stress biomarkers
OT  - Reference change value
OT  - T2DM
EDAT- 2015/02/05 06:00
MHDA- 2015/02/05 06:01
PMCR- 2015/02/02
CRDT- 2015/02/05 06:00
PHST- 2014/06/20 00:00 [received]
PHST- 2015/01/03 00:00 [accepted]
PHST- 2015/02/05 06:00 [entrez]
PHST- 2015/02/05 06:00 [pubmed]
PHST- 2015/02/05 06:01 [medline]
PHST- 2015/02/02 00:00 [pmc-release]
AID - 15 [pii]
AID - 10.1186/s12575-015-0015-9 [doi]
PST - epublish
SO  - Biol Proced Online. 2015 Feb 2;17(1):3. doi: 10.1186/s12575-015-0015-9. 
      eCollection 2015.