PMID- 25649907
OWN - NLM
STAT- MEDLINE
DCOM- 20150422
LR  - 20181202
IS  - 1365-2613 (Electronic)
IS  - 0959-9673 (Print)
IS  - 0959-9673 (Linking)
VI  - 96
IP  - 1
DP  - 2015 Feb
TI  - Double labelling of human umbilical cord mesenchymal stem cells with Gd-DTPA and 
      PKH26 and the influence on biological characteristics of hUCMSCs.
PG  - 63-72
LID - 10.1111/iep.12111 [doi]
AB  - The aim of this study was to determine whether double labelling of human 
      umbilical cord mesenchymal stem cells (hUCMSCs) with gadolinium-diethylene 
      triamine penta-acetic acid (Gd-DTPA) and PKH26 influences their biological 
      characteristics. A tissue adherence technique was used to separate and purify the 
      hUCMSCs and flow cytometry was performed to detect the surface markers expressed 
      on them. Gd-DTPA and PKH26 were used to label the stem cells and MRI and 
      fluorescence microscopy were used to detect the double-labelled hUCMSCs. A MTT 
      assay was used to delineate the growth curve. Transmission electron microscopy 
      (TEM) and atomic force microscopy were used to demonstrate the ultrastructural 
      features of the hUCMSCs. Flow cytometry showed that hUCMSCs highly expressed 
      CD29, CD90, CD44 and CD105. No expression of CD31, CD34 and CD45 was detected. 
      Very low expression of HLA-DR and CD40 was detected. Atomic force microscopy 
      showed these cells were long, spindle shaped, and the cytoplasm and nucleus had 
      clear boundaries. After double labelling, TEM showed Gd particles aggregated in 
      the cytoplasm in a cluster pattern. The proliferation activity, cell cycle, 
      apoptosis and differentiation of the stem cells were not influenced by double 
      labelling. Thus a tissue adherence technique is helpful to separate and purify 
      hUCMSCs effectively; and Gd-DTPA and PKH26 are promising tracers in the 
      investigation of migration and distribution of hUCMSCs in vivo.
CI  - (c) 2015 The Authors. International Journal of Experimental Pathology (c) 2015 
      International Journal of Experimental Pathology.
FAU - Shuai, Hanlin
AU  - Shuai H
AD  - Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinan 
      University, Guangzhou, China.
FAU - Shi, Changzheng
AU  - Shi C
FAU - Lan, Jifa
AU  - Lan J
FAU - Chen, Danliang
AU  - Chen D
FAU - Luo, Xin
AU  - Luo X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150204
PL  - England
TA  - Int J Exp Pathol
JT  - International journal of experimental pathology
JID - 9014042
RN  - 0 (Biomarkers)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Organic Chemicals)
RN  - 0 (PKH 26)
RN  - 0 (Radiopharmaceuticals)
RN  - K2I13DR72L (Gadolinium DTPA)
SB  - IM
MH  - Apoptosis
MH  - Biomarkers/metabolism
MH  - Cell Adhesion
MH  - Cell Cycle
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Cell Separation/methods
MH  - Cell Shape
MH  - Cell Tracking/*methods
MH  - Cells, Cultured
MH  - Flow Cytometry
MH  - *Fluorescent Dyes
MH  - *Gadolinium DTPA
MH  - Humans
MH  - *Magnetic Resonance Imaging
MH  - Mesenchymal Stem Cells/metabolism/*physiology/ultrastructure
MH  - Microscopy, Atomic Force
MH  - Microscopy, Confocal
MH  - Microscopy, Electron, Transmission
MH  - *Microscopy, Fluorescence
MH  - *Organic Chemicals
MH  - *Radiopharmaceuticals
MH  - Umbilical Cord/*cytology
PMC - PMC4352353
OTO - NOTNLM
OT  - Gd-DTPA
OT  - PKH26
OT  - Tracing
OT  - magnetic resonance imaging
OT  - mesenchymal stem cells
EDAT- 2015/02/05 06:00
MHDA- 2015/04/23 06:00
PMCR- 2016/02/01
CRDT- 2015/02/05 06:00
PHST- 2014/02/25 00:00 [received]
PHST- 2014/10/31 00:00 [accepted]
PHST- 2015/02/05 06:00 [entrez]
PHST- 2015/02/05 06:00 [pubmed]
PHST- 2015/04/23 06:00 [medline]
PHST- 2016/02/01 00:00 [pmc-release]
AID - 10.1111/iep.12111 [doi]
PST - ppublish
SO  - Int J Exp Pathol. 2015 Feb;96(1):63-72. doi: 10.1111/iep.12111. Epub 2015 Feb 4.