PMID- 25649939
OWN - NLM
STAT- MEDLINE
DCOM- 20151015
LR  - 20181113
IS  - 1347-7439 (Electronic)
IS  - 0916-7250 (Print)
IS  - 0916-7250 (Linking)
VI  - 76
IP  - 12
DP  - 2014 Dec
TI  - Molecular detection of avian pathogens in poultry red mite (Dermanyssus gallinae) 
      collected in chicken farms.
PG  - 1583-7
LID - 10.1292/jvms.14-0253 [doi]
AB  - Poultry red mite (PRM, Dermanyssus gallinae) is a blood-sucking ectoparasite as 
      well as a possible vector of several avian pathogens. In this study, to define 
      the role of PRM in the prevalence of avian infectious agents, we used polymerase 
      chain reaction (PCR) to check for the presence of seven pathogens: Avipox virus 
      (APV), Fowl Adenovirus (FAdV), Marek's disease virus (MDV), Erysipelothrix 
      rhusiopathiae (ER), Salmonella enterica (SE), Mycoplasma synoviae (MS) and 
      Mycoplasma gallisepticum (MG). A total of 159 PRM samples collected between 2004 
      and 2012 from 142 chicken farms in 38 prefectures in Japan were examined. APV DNA 
      was detected in 22 samples (13.8%), 19 of which were wild-type APV. 16S ribosomal 
      RNA (16S rRNA) of MS was detected in 15 samples (9.4%), and the mgc2 gene of MG 
      was detected in 2 samples (1.3%). Eight of 15 MS 16S rRNA sequences differed from 
      the vaccine sequence, indicating they were wild-type strains, while both of the 
      MG mgc2 gene sequences detected were identical to the vaccine sequences. Of these 
      avian pathogen-positive mite samples, three were positive for both wild-types of 
      APV and MS. On the other hand, the DNAs of ER, SE, FAdV and MDV were not detected 
      in any samples. These findings indicated that PRM can harbor the wild-type 
      pathogens and might play a role as a vector in spreading these diseases in farms.
FAU - Huong, Chu Thi Thanh
AU  - Huong CT
AD  - Laboratory of Veterinary Hygiene, Department of Veterinary Medicine, Faculty of 
      Agriculture, Tottori University, Koyama Minami 4-101, Tottori 680-8553, Japan.
FAU - Murano, Takako
AU  - Murano T
FAU - Uno, Yukiko
AU  - Uno Y
FAU - Usui, Tatsufumi
AU  - Usui T
FAU - Yamaguchi, Tsuyoshi
AU  - Yamaguchi T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140908
PL  - Japan
TA  - J Vet Med Sci
JT  - The Journal of veterinary medical science
JID - 9105360
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Viral)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - Arachnid Vectors/*microbiology/*virology
MH  - Avipoxvirus/genetics
MH  - Base Sequence
MH  - Chickens/*parasitology
MH  - DNA, Bacterial/*isolation & purification
MH  - DNA, Viral/*isolation & purification
MH  - Erysipelothrix/genetics
MH  - Fowl adenovirus A/genetics
MH  - Japan/epidemiology
MH  - Mardivirus/genetics
MH  - Mites/*microbiology/*virology
MH  - Molecular Sequence Data
MH  - Mycoplasma gallisepticum/genetics
MH  - Mycoplasma synoviae/genetics
MH  - Polymerase Chain Reaction/veterinary
MH  - RNA, Ribosomal, 16S/genetics
MH  - Salmonella enterica/genetics
MH  - Sequence Analysis, DNA
MH  - Sequence Homology
PMC - PMC4300372
EDAT- 2015/02/05 06:00
MHDA- 2015/10/16 06:00
PMCR- 2014/12/01
CRDT- 2015/02/05 06:00
PHST- 2015/02/05 06:00 [entrez]
PHST- 2015/02/05 06:00 [pubmed]
PHST- 2015/10/16 06:00 [medline]
PHST- 2014/12/01 00:00 [pmc-release]
AID - 14-0253 [pii]
AID - 10.1292/jvms.14-0253 [doi]
PST - ppublish
SO  - J Vet Med Sci. 2014 Dec;76(12):1583-7. doi: 10.1292/jvms.14-0253. Epub 2014 Sep 
      8.