PMID- 25649981
OWN - NLM
STAT- MEDLINE
DCOM- 20160209
LR  - 20190923
IS  - 1738-8872 (Electronic)
IS  - 1017-7825 (Linking)
VI  - 25
IP  - 5
DP  - 2015 May
TI  - 15-Hydroxyeicosatetraenoic Acid Inhibits Phorbol-12-Myristate-13-Acetate-Induced 
      MUC5AC Expression in NCI-H292 Respiratory Epithelial Cells.
PG  - 589-97
AB  - It has been reported that overexpression of MUC5AC induced by excessive 
      inflammation leads to airway obstruction in respiratory diseases such as chronic 
      obstructive pulmonary disease and asthma. 15-Hydroxyeicosatetraenoic acid 
      (15-HETE) has been reported to have anti-inflammatory effects, but the role of 
      15-HETE in respiratory inflammation has not been determined. Therefore, the aim 
      of this study was to investigate the effects of 15-HETE on MUC5AC expression and 
      related pathways. In this study, phorbol-12-myristate-13-acetate (PMA) was used 
      to stimulate NCI-H292 bronchial epithelial cells in order to examine the effects 
      of 15-HETE. 15-HETE inhibited PMA-induced expression of MUC5AC mRNA and secretion 
      of MUC5AC protein. Also, 15-HETE regulated matrix metallopeptidase 9 (MMP-9), 
      mitogen-activated protein kinase kinase (MEK) and extracellular signal-regulated 
      kinase (ERK). In addition, 15-HETE decreased translocation of specificity 
      protein-1 (Sp-1) transcription factor and nuclear factor kappaB (NF-kappaB) into 
      nuclear. Furthermore, 15-HETE enhanced transcriptional activity of peroxisome 
      proliferator-activated receptor gamma (PPARgamma) as a PPARgamma agonist. This activity 
      reduced phosphorylation of protein kinase B (PKappaB/Akt) by increasing the 
      expression of phosphatase and tensin homolog (PTEN). In conclusion, 15-HETE 
      regulated MUC5AC expression via modulating MMP-9, MEK/ERK/Sp-1 and PPARgamma/PTEN/ 
      Akt signaling pathways in PMA-treated respiratory epithelial cells.
FAU - Song, Yong-Seok
AU  - Song YS
AD  - Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, 
      Konkuk University, Seoul 143-701, Republic of Korea.
FAU - Kim, Man Sub
AU  - Kim MS
AD  - Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, 
      Konkuk University, Seoul 143-701, Republic of Korea.
FAU - Lee, Dong Hun
AU  - Lee DH
AD  - Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, 
      Konkuk University, Seoul 143-701, Republic of Korea.
FAU - Oh, Doek-Kun
AU  - Oh DK
AD  - Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, 
      Konkuk University, Seoul 143-701, Republic of Korea.
FAU - Yoon, Do-Young
AU  - Yoon DY
AD  - Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, 
      Konkuk University, Seoul 143-701, Republic of Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Korea (South)
TA  - J Microbiol Biotechnol
JT  - Journal of microbiology and biotechnology
JID - 9431852
RN  - 0 (MUC5AC protein, human)
RN  - 0 (Mucin 5AC)
RN  - 0 (Phorbol Esters)
RN  - 20839-06-9 (phorbol-12-myristate)
RN  - 97850-14-1 (15-hydroxy-5,8,11,13,17-eicosapentaenoic acid)
RN  - AAN7QOV9EA (Eicosapentaenoic Acid)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Cell Line, Tumor
MH  - Eicosapentaenoic Acid/*analogs & derivatives/pharmacology
MH  - Humans
MH  - MAP Kinase Signaling System/drug effects
MH  - Matrix Metalloproteinase 9/metabolism
MH  - Models, Biological
MH  - Mucin 5AC/genetics/*metabolism
MH  - Phorbol Esters/*pharmacology
MH  - Respiratory Mucosa/cytology/*drug effects
EDAT- 2015/02/05 06:00
MHDA- 2016/02/10 06:00
CRDT- 2015/02/05 06:00
PHST- 2015/02/05 06:00 [entrez]
PHST- 2015/02/05 06:00 [pubmed]
PHST- 2016/02/10 06:00 [medline]
AID - 10.4014/jmb.1501.01052 [pii]
AID - 10.4014/jmb.1501.01052 [doi]
PST - ppublish
SO  - J Microbiol Biotechnol. 2015 May;25(5):589-97. doi: 10.4014/jmb.1501.01052.