PMID- 25650415
OWN - NLM
STAT- MEDLINE
DCOM- 20150903
LR  - 20181113
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 56
IP  - 3
DP  - 2015 Feb 3
TI  - Aqueous cell differentiation in anterior uveitis using Fourier-domain optical 
      coherence tomography.
PG  - 1430-6
LID - 10.1167/iovs.14-15118 [doi]
AB  - PURPOSE: The differential diagnosis of a patient presenting with anterior uveitis 
      is broad and can present a diagnostic challenge. In this study, we evaluate the 
      characteristic findings of inflammatory cells on optical coherence tomography 
      (OCT) both in vitro and in vivo. METHODS: Blood from two healthy volunteers was 
      prepared using standardized methods for cell sorting with a flow cytometer 
      (FASCAria). Neutrophils, lymphocytes, monocytes, and red blood cells were placed 
      in suspension and scanned with a 26-kHz Fourier-domain OCT system (RTVue) with 
      5-mum axial resolution. Custom software algorithms were used to identify cells 
      based on their reflectance distribution. These algorithms were then applied to 
      OCT images obtained from uveitis patients with active anterior chamber 
      inflammation. RESULTS: On OCT images the cells appeared as hyperreflective spots. 
      In vitro, cell reflectance was statistically significantly different between all 
      of the cell types (neutrophils, monocytes, lymphocytes, and red blood cells, P < 
      0.001, Mann-Whitney test). In vivo, the relationship between underlying disease 
      and cell type imaged on OCT was highly statistically significant, with human 
      leukocyte antigen (HLA)-B27-associated uveitis patients having a predominantly 
      polymorphonuclear pattern on OCT and sarcoidosis and inflammatory bowel disease 
      patients having a predominantly mononuclear pattern on OCT (P < 0.001, Fisher's 
      exact test). CONCLUSIONS: These in vitro and in vivo data demonstrate the 
      potential of OCT to evaluate cells in the anterior chamber of patients 
      noninvasively. Optical coherence tomography may be a useful adjunct to guide the 
      diagnosis and treatment of ocular inflammatory conditions.
CI  - Copyright 2015 The Association for Research in Vision and Ophthalmology, Inc.
FAU - Rose-Nussbaumer, Jennifer
AU  - Rose-Nussbaumer J
AD  - Casey Eye Institute, Oregon Health and Science University, Portland, Oregon, 
      United States.
FAU - Li, Yan
AU  - Li Y
AD  - Casey Eye Institute, Oregon Health and Science University, Portland, Oregon, 
      United States.
FAU - Lin, Phoebe
AU  - Lin P
AD  - Casey Eye Institute, Oregon Health and Science University, Portland, Oregon, 
      United States.
FAU - Suhler, Eric
AU  - Suhler E
AD  - Casey Eye Institute, Oregon Health and Science University, Portland, Oregon, 
      United States.
FAU - Asquith, Mark
AU  - Asquith M
AD  - Casey Eye Institute, Oregon Health and Science University, Portland, Oregon, 
      United States.
FAU - Rosenbaum, James T
AU  - Rosenbaum JT
AD  - Casey Eye Institute, Oregon Health and Science University, Portland, Oregon, 
      United States.
FAU - Huang, David
AU  - Huang D
AD  - Casey Eye Institute, Oregon Health and Science University, Portland, Oregon, 
      United States.
LA  - eng
GR  - R01 EY018184/EY/NEI NIH HHS/United States
GR  - R01EY018184/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20150203
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
SB  - IM
MH  - Adult
MH  - Aged
MH  - Algorithms
MH  - Aqueous Humor/*cytology
MH  - Erythrocytes/physiology
MH  - Female
MH  - *Flow Cytometry
MH  - *Fourier Analysis
MH  - Humans
MH  - *Image Interpretation, Computer-Assisted
MH  - In Vitro Techniques
MH  - Lymphocytes/physiology
MH  - Male
MH  - Middle Aged
MH  - Monocytes/physiology
MH  - Neutrophils/physiology
MH  - Software
MH  - *Tomography, Optical Coherence
MH  - Uveitis, Anterior/*diagnosis/*physiopathology
MH  - Young Adult
PMC - PMC4347308
OTO - NOTNLM
OT  - Fourier-domain optical coherence tomography
OT  - anterior uveitis
OT  - image analysis
OT  - mononuclear leukocytes
OT  - polymorphonuclear leukocytes
EDAT- 2015/02/05 06:00
MHDA- 2015/09/04 06:00
PMCR- 2015/09/01
CRDT- 2015/02/05 06:00
PHST- 2015/02/05 06:00 [entrez]
PHST- 2015/02/05 06:00 [pubmed]
PHST- 2015/09/04 06:00 [medline]
PHST- 2015/09/01 00:00 [pmc-release]
AID - iovs.14-15118 [pii]
AID - IOVS-14-15118 [pii]
AID - 10.1167/iovs.14-15118 [doi]
PST - epublish
SO  - Invest Ophthalmol Vis Sci. 2015 Feb 3;56(3):1430-6. doi: 10.1167/iovs.14-15118.