PMID- 2565181
OWN - NLM
STAT- MEDLINE
DCOM- 19890605
LR  - 20200825
IS  - 0742-8413 (Print)
IS  - 0742-8413 (Linking)
VI  - 92
IP  - 2
DP  - 1989
TI  - Excitotoxicity, reflex responses, and evoked changes in extracellular potassium 
      in the frog spinal cord.
PG  - 205-10
AB  - 1. The effects of the excitatory amino acid agonists kainate (KA), quisqualate 
      (QUIS), and N-methyl-D-aspartate (NMDA) were studied in vitro on the hemisected 
      frog spinal cord. 2. Prolonged (1.0 hr) application of excitatory amino acid 
      agonists (KA, 50 or 300 microM; QUIS, 30 microM; NMDA, 300 microM) significantly 
      reduced the ventral root potentials (VRPs) and [K+]0 evoked by a dorsal root 
      tetanus (10 sec, 25 Hz), by brief (10 sec) applications of the same agonists (KA, 
      30 microM; QUIS, 30 microM; NMDA, 300 microM), and by GABA (10 sec, 1.0 mM). 3. 
      The effect was essentially irreversible and persisted despite 2-4 hr of washing. 
      4. Excitatory amino acid antagonists (APV, 30 microM and kynurenate, 2 mM) 
      blocked the neurotoxic effects of the excitatory agonists NMDA and KA 
      respectively, an observation which indicates the observed effects of the agonists 
      require the activation of specific excitatory receptors. 5. TTX did not alter the 
      neurotoxic effects of KA suggesting that interneuronal firing does not contribute 
      to the observed changes. 6. Addition of high K+ did not duplicate the effect of 
      prolonged excitatory amino acid agonist exposure, an indication that elevation of 
      K+ does not cause the decreased responses. 7. Light microscopy did not provide 
      any evidence of gross tissue damage. 8. The parallel reduction of postsynaptic 
      responses and delta [K+]0 support the idea that elevation of extracellular [K+] 
      by afferent stimuli results from interneuronal activity.
FAU - Zhang, D X
AU  - Zhang DX
AD  - Department of Neurology, University of Miami School of Medicine, FL.
FAU - Hackman, J C
AU  - Hackman JC
FAU - Davidoff, R A
AU  - Davidoff RA
LA  - eng
GR  - NS 17577/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Comp Biochem Physiol C Comp Pharmacol Toxicol
JT  - Comparative biochemistry and physiology. C, Comparative pharmacology and 
      toxicology
JID - 8310013
RN  - 0 (Amino Acids)
RN  - 4368-28-9 (Tetrodotoxin)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
RN  - RWP5GA015D (Potassium)
SB  - IM
MH  - Amino Acids/*pharmacology
MH  - Animals
MH  - In Vitro Techniques
MH  - Potassium/*metabolism
MH  - Rana pipiens
MH  - Reflex/drug effects
MH  - Spinal Cord/*drug effects/metabolism/physiology
MH  - Tetrodotoxin/pharmacology
MH  - gamma-Aminobutyric Acid/pharmacology
EDAT- 1989/01/01 00:00
MHDA- 1989/01/01 00:01
CRDT- 1989/01/01 00:00
PHST- 1989/01/01 00:00 [pubmed]
PHST- 1989/01/01 00:01 [medline]
PHST- 1989/01/01 00:00 [entrez]
AID - 10.1016/0742-8413(89)90042-x [doi]
PST - ppublish
SO  - Comp Biochem Physiol C Comp Pharmacol Toxicol. 1989;92(2):205-10. doi: 
      10.1016/0742-8413(89)90042-x.