PMID- 25652596
OWN - NLM
STAT- MEDLINE
DCOM- 20160517
LR  - 20150812
IS  - 1932-846X (Electronic)
IS  - 1932-8451 (Linking)
VI  - 75
IP  - 9
DP  - 2015 Sep
TI  - A proteomic approach to understand MMP-3-driven developmental processes in the 
      postnatal cerebellum: Chaperonin CCT6A and MAP kinase as contributing factors.
PG  - 1033-48
LID - 10.1002/dneu.22272 [doi]
AB  - Matrix metalloproteinase-3 (MMP-3) deficiency in mice was previously reported to 
      result in a transiently retarded granule cell migration at postnatal day 8 (P8) 
      and a sustained disturbed arborization of Purkinje cell dendrites from P8 on, 
      concomitant with a delayed synapse formation between granule cells and Purkinje 
      cells and resulting in mild deficits in motor performance in adult animals. 
      However, the molecular mechanisms by which MMP-3 contributes to proper 
      development of the cerebellar cortex during the first postnatal weeks remains 
      unknown. In this study, we used a functional proteomics approach to investigate 
      alterations in protein expression in postnatal cerebella of wild-type versus 
      MMP-3 deficient mice, and to further elucidate MMP-3-dependent pathways and 
      downstream targets in vivo. At P8, two-dimensional difference gel electrophoresis 
      and mass spectrometry identified 20 unique proteins with a different expression 
      between the two genotypes. Subsequent "Ingenuity Pathway Analysis" and Western 
      blotting indicate that the chaperonin containing T-complex polypeptide 1, subunit 
      6A and the MAP kinase signaling pathway play a key role in the MMP-3-dependent 
      regulation of neurite outgrowth and neuronal migration in the developing brain.
CI  - (c) 2015 Wiley Periodicals, Inc.
FAU - Van Hove, Inge
AU  - Van Hove I
AD  - Laboratory of Neural Circuit Development and Regeneration, Animal Physiology and 
      Neurobiology Section, Department of Biology, KU Leuven, Leuven, Belgium.
FAU - Verslegers, Mieke
AU  - Verslegers M
AD  - Laboratory of Neural Circuit Development and Regeneration, Animal Physiology and 
      Neurobiology Section, Department of Biology, KU Leuven, Leuven, Belgium.
FAU - Hu, Tjing-Tjing
AU  - Hu TT
AD  - Laboratory of Neuroplasticity and Neuroproteomics, Animal Physiology and 
      Neurobiology Section, Department of Biology, KU Leuven, Leuven, Belgium.
FAU - Carden, Martin
AU  - Carden M
AD  - School of Biosciences, University of Kent, Canterbury, CT2 7NJ, United Kingdom.
FAU - Arckens, Lutgarde
AU  - Arckens L
AD  - Laboratory of Neuroplasticity and Neuroproteomics, Animal Physiology and 
      Neurobiology Section, Department of Biology, KU Leuven, Leuven, Belgium.
FAU - Moons, Lieve
AU  - Moons L
AD  - Laboratory of Neural Circuit Development and Regeneration, Animal Physiology and 
      Neurobiology Section, Department of Biology, KU Leuven, Leuven, Belgium.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150218
PL  - United States
TA  - Dev Neurobiol
JT  - Developmental neurobiology
JID - 101300215
RN  - 0 (CCT6A protein, mouse)
RN  - EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - EC 3.4.24.17 (Mmp3 protein, mouse)
RN  - EC 3.6.1.- (Chaperonin Containing TCP-1)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Blotting, Western
MH  - Cerebellum/*growth & development/*metabolism
MH  - Chaperonin Containing TCP-1/*metabolism
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Fluorescent Antibody Technique
MH  - MAP Kinase Signaling System/physiology
MH  - Mass Spectrometry
MH  - Matrix Metalloproteinase 3/genetics/*metabolism
MH  - Mice, Knockout
MH  - Mitogen-Activated Protein Kinase Kinases/*metabolism
MH  - Proteomics
OTO - NOTNLM
OT  - MAP kinase
OT  - cerebellum
OT  - chaperonin containing TCP-1
OT  - development
OT  - matrix metalloproteinase-3
EDAT- 2015/02/06 06:00
MHDA- 2016/05/18 06:00
CRDT- 2015/02/06 06:00
PHST- 2014/09/24 00:00 [received]
PHST- 2015/01/16 00:00 [revised]
PHST- 2015/01/16 00:00 [accepted]
PHST- 2015/02/06 06:00 [entrez]
PHST- 2015/02/06 06:00 [pubmed]
PHST- 2016/05/18 06:00 [medline]
AID - 10.1002/dneu.22272 [doi]
PST - ppublish
SO  - Dev Neurobiol. 2015 Sep;75(9):1033-48. doi: 10.1002/dneu.22272. Epub 2015 Feb 18.