PMID- 25652828
OWN - NLM
STAT- MEDLINE
DCOM- 20160211
LR  - 20150521
IS  - 1559-0291 (Electronic)
IS  - 0273-2289 (Linking)
VI  - 176
IP  - 1
DP  - 2015 May
TI  - A lentiviral vector visualizing the germ cell specification in vitro under the 
      control of Figla promoter.
PG  - 66-75
LID - 10.1007/s12010-015-1523-4 [doi]
AB  - Premature ovarian failure (POF) is affecting more and more women, which is the 
      loss of function of the ovaries before age 40. To elucidate the underlying 
      mechanisms of the oogenesis is of importance to understand the causes of impaired 
      fertility and POF. However, mammalian oogenesis in vivo is a complex process. 
      Thus, building an oogenesis visualizing system is beneficial for the study of 
      oogenesis. In this study, we found that Figla is specifically expressed in female 
      mice oocyte. Then, we constructed a lentiviral vector (pTRIP-Figla-EGFP-puro) 
      under the control of Figla promoter, which drived enhanced green fluorescent 
      protein (EGFP) as an indicator and used the lentiviral vector transduction the 
      ovarian cells and induced germ cells derived from human umbilical cord 
      mesenchymal stem cells (hUC-MSCs), and the results showed that the lentiviral 
      vector we constructed was able to specifically express green fluorescent protein 
      (GFP) in the ovarian oocyte and induced oocyte-like cells derived from hUC-MSCs, 
      which was Figla-positive cells. These results suggest that pTRIP-Figla-EGFP 
      vector provides a new system to study the role of Figla in oogenesis, and an 
      approach to study the development and the differentiation of germ cells derived 
      from stem cells.
FAU - Chu, Zhili
AU  - Chu Z
AD  - College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering and 
      Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China.
FAU - Niu, Bowen
AU  - Niu B
FAU - Li, Na
AU  - Li N
FAU - Hu, Yue
AU  - Hu Y
FAU - Li, Jian
AU  - Li J
FAU - Yu, Ping
AU  - Yu P
FAU - Wu, Chongyang
AU  - Wu C
FAU - Yan, Xinrong
AU  - Yan X
FAU - Lei, Anmin
AU  - Lei A
FAU - Hua, Jinlian
AU  - Hua J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150205
PL  - United States
TA  - Appl Biochem Biotechnol
JT  - Applied biochemistry and biotechnology
JID - 8208561
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (Figla protein, mouse)
RN  - 0 (enhanced green fluorescent protein)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - *Basic Helix-Loop-Helix Transcription Factors/genetics/metabolism
MH  - Cell Tracking/*methods
MH  - Female
MH  - *Genetic Vectors
MH  - *Green Fluorescent Proteins/biosynthesis/genetics
MH  - Humans
MH  - *Lentivirus
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Oocytes/cytology/*metabolism
MH  - Oogenesis/physiology
MH  - *Promoter Regions, Genetic
EDAT- 2015/02/06 06:00
MHDA- 2016/02/13 06:00
CRDT- 2015/02/06 06:00
PHST- 2014/10/05 00:00 [received]
PHST- 2015/01/21 00:00 [accepted]
PHST- 2015/02/06 06:00 [entrez]
PHST- 2015/02/06 06:00 [pubmed]
PHST- 2016/02/13 06:00 [medline]
AID - 10.1007/s12010-015-1523-4 [doi]
PST - ppublish
SO  - Appl Biochem Biotechnol. 2015 May;176(1):66-75. doi: 10.1007/s12010-015-1523-4. 
      Epub 2015 Feb 5.